| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KRT3 |
| Uniprot No | P12035 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-628aa |
| 氨基酸序列 | MSRQASKTSG GGSQGFSGRS AVVSGSSRMS CVAHSGGAGG GAYGFRSGAG GFGSRSLYNL GGNKSISISV AAGGSRAGGF GGGRSSCAFA GGYGGGFGSG YGGGFGGGFG GGRGMGGGFG GAGGFGGAGG FGGAGGFGGP GGFGGSGGFG GPGSLGSPGG FGPGGFPGGI QEVTINQSLL QPLNVEIDPQ IGQVKAQERE QIKTLNNKFA SFIDKVRFLE QQNKVLETKW NLLQQQGTSS ISGTNNLEPL FENHINYLRS YLDNILGERG RLDSELKNME DLVEDFKKKY EDEINKRTAA ENEFVTLKKD VDSAYMNKVE LQAKVDALID EIDFLRTLYD AELSQMQSHI SDTSVVLSMD NNRSLDLDSI IAEVRAQYED IAQRSKAEAE ALYQTKLGEL QTTAGRHGDD LRNTKSEIIE LNRMIQRLRA EIEGVKKQNA NLQTAIAEAE QHGEMALKDA NAKLQELQAA LQQAKDDLAR LLRDYQELMN VKLALDVEIA TYRKLLEGEE YRMSGECPSA VSISVVSSST TSASAGGYGG GYGGGMGGGL GGGFSAGGGS GSGFGRGGGG GIGGGFGGGS SGFSGGSGFG SISGARYGVS GGGFSSASNR GGSIKFSQSS QSSQRYSR |
| 预测分子量 | 64,4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于KRT3重组蛋白的模拟参考文献示例(注：部分内容为虚构，仅作格式参考，建议通过学术数据库核实具体文献)：
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1. **文献名称**: *"Recombinant Expression and Characterization of Human Keratin 3 (KRT3) in Escherichia coli"*
**作者**: Smith JL, et al.
**摘要**: 本研究成功在大肠杆菌中表达了重组人KRT3蛋白，并通过亲和层析纯化。实验验证了重组KRT3的抗原性，为后续开发角膜疾病相关抗体提供了基础。
2. **文献名称**: *"Functional Analysis of KRT3 Mutations in Corneal Dystrophy Using Recombinant Proteins"*
**作者**: Chen R, et al.
**摘要**: 通过体外表达KRT3重组蛋白，研究了其突变体(如R95C)对蛋白稳定性和角膜上皮细胞结构的影响，揭示了突变导致角膜营养不良的分子机制。
3. **文献名称**: *"Production of Recombinant KRT3 in Insect Cells for Structural Studies"*
**作者**: Tanaka M, et al.
**摘要**: 利用杆状病毒-昆虫细胞系统表达重组KRT3蛋白，结合X射线晶体学分析其三维结构，阐明了KRT3与角膜特异性角蛋白网络形成的关键结构域。
4. **文献名称**: *"KRT3 Recombinant Protein as a Biomarker for Corneal Epithelial Integrity"*
**作者**: Gupta S, et al.
**摘要**: 研究评估了重组KRT3蛋白在体外模型中对上皮细胞粘附的调控作用，提出其作为评估角膜损伤修复潜力的生物标志物。
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**建议**：以上为示例性内容，实际文献需通过PubMed、Google Scholar等平台检索关键词(如“KRT3 recombinant protein”、“keratin 3 expression”)获取。若研究较少，可扩展至KRT3功能或相关疾病机制的文献。
**Background of Recombinant KRT3 Protein**
Keratin 3 (KRT3) is a type I intermediate filament protein encoded by the *KRT3* gene, primarily co-expressed with its type II partner, keratin 12 (KRT12), in the corneal epithelium. These proteins form a cytoskeletal network critical for maintaining the structural integrity and mechanical resilience of corneal epithelial cells. KRT3-KRT12 heterodimers assemble into filaments that resist cellular stress, protect against damage, and regulate cell signaling. Mutations in *KRT3* or *KRT12* are linked to Meesmann epithelial corneal dystrophy (MECD), a rare genetic disorder characterized by fragile corneal epithelium, microcysts, and photophobia.
Recombinant KRT3 protein is produced using biotechnological platforms, such as bacterial (*E. coli*) or mammalian expression systems, followed by purification via affinity chromatography. Its production enables detailed study of keratin function, interactions, and disease mechanisms. Researchers employ recombinant KRT3 to investigate corneal epithelial biology, model MECD *in vitro*, and screen therapeutic compounds aimed at stabilizing keratin networks.
In biomedical research, recombinant KRT3 serves as a tool to explore cellular stress responses, epithelial differentiation, and wound healing. It is also used to generate antibodies for diagnostic applications and to validate gene-editing strategies targeting keratinopathies. By providing a controlled, scalable source of functional protein, recombinant KRT3 advances our understanding of corneal diseases and supports the development of targeted therapies to restore epithelial homeostasis.
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