| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KRT28 |
| Uniprot No | Q7Z3Y7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-464aa |
| 氨基酸序列 | MSLQFSNGSR HVCLRSGAGS VRPLNGGAGF AGSSACGGSV AGSEFSCALG GGLGSVPGGS HAGGALGNAA CIGFAGSEGG LLSGNEKVTM QNLNDRLASY LDNVRALEEA NAELERKIKG WYEKYGPGSC RGLDHDYSRY HLTIEDLKNK IISSTTTNAN VILQIDNARL AADDFRLKYE NELTLHQNVE ADINGLRRVL DELTLCRTDQ ELQYESLSEE MTYLKKNHEE EMKALQCAAG GNVNVEMNAA PGVDLAVLLN NMRAEYEALA EQNRKDAEAW FNEKSASLQQ QISHDSGAAT FARSQLTEMR RTLQTLEIQL QSLMATKHSL ECSLTETESN YCTQLAQIQA QIGALEEQLH QVRTETEGQK LEYEHLLDVK VHLEKEIETY CRLIDGDGNS CSKSKGFGSG SPGNSSKDLS KTTLVKTVVE ELDQRGKVLS SRIHSIEEKT SKMTNGKTEQ RVPF |
| 预测分子量 | 50,5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于KRT28重组蛋白的模拟参考文献示例，基于角蛋白相关研究的常见方向整理：
---
1. **标题**：*"Recombinant Expression and Structural Characterization of Human Keratin 28 in a Eukaryotic System"*
**作者**：Zhang Y. et al.
**期刊**：*Protein Expr. Purif.* 2021.
**摘要**：本研究成功利用杆状病毒-昆虫细胞系统表达了重组KRT28蛋白，并通过质谱和圆二色光谱验证其正确折叠及二级结构特征，为后续功能研究提供了高纯度蛋白样品。
2. **标题**：*"KRT28 Recombinant Protein Modulates Epithelial-Mesenchymal Transition in Colorectal Cancer Cells"*
**作者**：Li X. et al.
**期刊**：*Oncol. Rep.* 2019.
**摘要**：通过体外实验证实，重组KRT28蛋白能抑制结直肠癌细胞迁移和侵袭，其机制可能与调控E-cadherin/β-catenin信号通路有关，提示其在肿瘤转移中的潜在作用。
3. **标题**：*"Development of Monoclonal Antibodies Against KRT28 Using Recombinant Antigen"*
**作者**：Chen L. et al.
**期刊**：*J. Immunol. Methods* 2020.
**摘要**：利用大肠杆菌表达的重组KRT28蛋白作为免疫原，成功制备出特异性单克隆抗体，并通过免疫组化验证其在皮肤组织中的染色应用。
4. **标题**：*"Functional Analysis of KRT28 Mutants in Hereditary Hair Disorders via Recombinant Protein Reconstitution"*
**作者**：Wang R. et al.
**期刊**：*J. Invest. Dermatol.* 2022.
**摘要**：通过重组KRT28突变体蛋白的体外组装实验，揭示了特定氨基酸替换导致角蛋白纤维稳定性降低，为遗传性毛发疾病的分子机制提供了新见解。
---
**注**：以上文献为模拟生成，实际研究中建议通过PubMed、Web of Science等数据库检索具体文章。若需真实文献，可提供更详细的关键词或研究方向进一步筛选。
**Background of KRT28 Recombinant Protein**
Keratin 28 (KRT28) is a member of the keratin protein family, which constitutes a critical component of the intermediate filament cytoskeleton in epithelial cells. Keratins are broadly categorized into type I (acidic) and type II (neutral-basic) proteins, forming heteropolymers to provide mechanical resilience, regulate cell signaling, and maintain structural integrity in tissues. KRT28. classified as a type I keratin, is primarily expressed in specific epithelial tissues, though its exact distribution and functional specialization remain less characterized compared to other well-studied keratins like KRT5 or KRT14.
The interest in KRT28 stems from its potential role in epithelial differentiation and pathology. Dysregulation of keratins is linked to diseases such as cancer, blistering disorders, and fibrosis. For instance, altered keratin expression patterns are biomarkers for epithelial-derived carcinomas. Recombinant KRT28 protein, produced via genetic engineering in systems like *E. coli* or mammalian cell cultures, enables researchers to study its biochemical properties, interactions, and post-translational modifications *in vitro*. This engineered protein retains native epitopes, making it valuable for antibody development, diagnostic assays, and mechanistic studies.
Current research focuses on elucidating KRT28's tissue-specific functions, its partnership with type II keratins, and its involvement in cellular stress responses. Recombinant KRT28 also aids in modeling keratinopathies and screening therapeutic compounds targeting keratin-related disorders. As a tool, it bridges gaps in understanding epithelial biology and disease mechanisms, highlighting its relevance in both basic and applied biomedical research.
×
