| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KRT40 |
| Uniprot No | Q6A162 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-431aa |
| 氨基酸序列 | MTSDCSSTHC SPESCGTASG CAPASSCSVE TACLPGTCAT SRCQTPSFLS RSRGLTGCLL PCYFTGSCNS PCLVGNCAWC EDGVFTSNEK ETMQFLNDRL ASYLEKVRSL EETNAELESR IQEQCEQDIP MVCPDYQRYF NTIEDLQQKI LCTKAENSRL AVQLDNCKLA TDDFKSKYES ELSLRQLLEA DISSLHGILE ELTLCKSDLE AHVESLKEDL LCLKKNHEEE VNLLREQLGD RLSVELDTAP TLDLNRVLDE MRCQCETVLA NNRREAEEWL AVQTEELNQQ QLSSAEQLQG CQMEILELKR TASALEIELQ AQQSLTESLE CTVAETEAQY SSQLAQIQCL IDNLENQLAE IRCDLERQNQ EYQVLLDVKA RLEGEINTYW GLLDSEDSRL SCSPCSTTCT SSNTCEPCSA YVICTVENCC L |
| 预测分子量 | 48,1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于KRT40重组蛋白的假设性参考文献示例(注：KRT40相关研究较少，实际文献需通过学术数据库验证)：
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1. **《Molecular cloning and characterization of human keratin 40》**
- **作者**: Smith A, et al.
- **摘要**: 本研究首次报道了人类KRT40基因的克隆及其重组蛋白在原核系统中的表达。通过体外功能实验发现，重组KRT40蛋白在维持上皮细胞结构完整性中起关键作用，并验证了其与KRT83的相互作用。
2. **《Recombinant keratin 40 expression in mammalian cells and its role in spermatogenesis》**
- **作者**: Zhang L, et al.
- **摘要**: 利用哺乳动物细胞系统成功表达重组KRT40蛋白，发现其在睾丸支持细胞中特异性高表达。通过基因敲除模型证实，KRT40缺失会导致精子发育异常，提示其在男性生殖系统中的功能重要性。
3. **《Structural analysis of keratin 40 through recombinant protein crystallography》**
- **作者**: Johnson R, et al.
- **摘要**: 采用昆虫细胞系统表达重组KRT40蛋白，首次解析了其晶体结构。研究发现其C端结构域具有独特的α-螺旋构象，为开发靶向角蛋白疾病的药物提供了结构基础。
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**注意**：以上文献为假设示例，实际研究中KRT40的报道较少，建议扩展检索以下方向：
- 使用更广泛的关键词如“keratin recombinant expression”或“type I keratin 40”;
- 查阅KRT40相关基因数据库(如UniProt或NCBI Gene)中的引用文献;
- 关注角蛋白家族综述论文中提及的KRT40研究线索。
Keratins are a family of intermediate filament proteins critical for maintaining structural integrity and mechanical stability in epithelial cells. As the largest subgroup of intermediate filaments, they are classified into Type I (acidic) and Type II (basic) keratins, which form heterodimers to assemble into functional filaments. KRT40 (keratin 40), a Type I keratin, is primarily expressed in specific epithelial tissues, including mucosal surfaces and stratified epithelia. Its expression pattern suggests roles in cellular differentiation, stress response, and tissue-specific barrier functions. Dysregulation of keratins like KRT40 has been linked to epithelial fragility disorders, chronic inflammation, and certain cancers, making it a potential biomarker or therapeutic target.
Recombinant KRT40 protein is engineered using expression systems like *E. coli* or mammalian cells, followed by purification via affinity chromatography (e.g., His-tag). This bioengineered tool retains structural and functional properties of native KRT40. enabling studies on keratin network dynamics, protein-protein interactions, and epithelial pathophysiology. Researchers utilize it to investigate mechanisms of keratin-related diseases, develop diagnostic antibodies, or screen compounds targeting keratin-associated pathways. Its availability accelerates research into epithelial biology and disorders such as mucosal injury or squamous cell carcinoma. Ongoing studies aim to clarify its tissue-specific roles and potential in regenerative medicine or personalized therapeutics.
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