| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KRT81 |
| Uniprot No | Q14533 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-505aa |
| 氨基酸序列 | MTCGSGFGGR AFSCISACGP RPGRCCITAA PYRGISCYRG LTGGFGSHSV CGGFRAGSCG RSFGYRSGGV CGPSPPCITT VSVNESLLTP LNLEIDPNAQ CVKQEEKEQI KSLNSRFAAF IDKVRFLEQQ NKLLETKLQF YQNRECCQSN LEPLFEGYIE TLRREAECVE ADSGRLASEL NHVQEVLEGY KKKYEEEVSL RATAENEFVA LKKDVDCAYL RKSDLEANVE ALIQEIDFLR RLYEEEILIL QSHISDTSVV VKLDNSRDLN MDCIIAEIKA QYDDIVTRSR AEAESWYRSK CEEMKATVIR HGETLRRTKE EINELNRMIQ RLTAEVENAK CQNSKLEAAV AQSEQQGEAA LSDARCKLAE LEGALQKAKQ DMACLIREYQ EVMNSKLGLD IEIATYRRLL EGEEQRLCEG IGAVNVCVSS SRGGVVCGDL CVSGSRPVTG SVCSAPCNGN VAVSTGLCAP CGQLNTTCGG GSCGVGSCGI SSLGVGSCGS SCRKC |
| 预测分子量 | 54,9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于KRT81重组蛋白的3篇参考文献示例(注：以下内容为虚构示例，仅用于演示格式)：
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1. **文献名称**：*Recombinant Expression and Functional Analysis of Human Keratin 81 (KRT81) in Bacterial Systems*
**作者**：Chen L, Wang Y, et al.
**摘要**：本研究成功在大肠杆菌中表达了KRT81重组蛋白，并通过亲和层析技术纯化获得高纯度蛋白。功能实验表明，重组KRT81在体外能够与细胞骨架蛋白相互作用，为研究其在毛发结构中的作用提供了基础。
2. **文献名称**：*KRT81 Recombinant Protein as a Biomarker for Hair Follicle Disorders*
**作者**：Rodriguez S, Kim H, et al.
**摘要**：通过哺乳动物细胞系统表达KRT81重组蛋白，并制备特异性抗体。研究发现，KRT81在遗传性毛发疾病患者的毛囊中表达异常，提示其可作为相关疾病的潜在诊断标志物。
3. **文献名称**：*Structural Characterization of Recombinant KRT81 Using Cryo-Electron Microscopy*
**作者**：Gupta R, Tanaka M, et al.
**摘要**：利用昆虫细胞系统表达KRT81重组蛋白，并通过冷冻电镜解析其三维结构。研究揭示了KRT81二聚体的关键结构域，为理解其机械稳定性和病理突变机制提供了新见解。
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如需真实文献，建议在PubMed、Google Scholar等平台检索关键词“KRT81 recombinant protein”或联系相关领域数据库获取。
Keratin 81 (KRT81) is a type II intermediate filament protein belonging to the keratin family, which plays critical structural and regulatory roles in epithelial cells. Encoded by the *KRT81* gene, this protein is primarily expressed in hair follicles, particularly in the cortex of hair shafts, where it contributes to the mechanical strength, elasticity, and overall integrity of hair. KRT81 forms heterodimers with type I keratins, such as KRT31 or KRT35. through coiled-coil interactions, creating a resilient cytoskeletal network essential for cellular stress resistance and tissue-specific functions.
Recombinant KRT81 protein is engineered using biotechnological platforms (e.g., bacterial, yeast, or mammalian expression systems) to produce purified, functional keratin for research and therapeutic applications. Its recombinant form enables precise study of keratinopathies—diseases linked to keratin mutations, such as monilethrix or hair shaft disorders—by allowing in vitro analysis of protein interactions, filament assembly, and mutation-induced structural defects. Researchers also utilize recombinant KRT81 to explore its role in wound healing, epithelial barrier maintenance, and hair follicle regeneration, given keratins' involvement in cell signaling and apoptosis regulation.
In dermatology and cosmetics, recombinant KRT81 serves as a model for developing hair-strengthening treatments or evaluating damage from chemical/thermal exposures. Additionally, its biocompatibility and biodegradability make it a candidate for biomedical materials, such as drug delivery scaffolds or tissue-engineered skin substitutes. Despite progress, challenges remain in replicating post-translational modifications seen in native KRT81. necessitating ongoing optimization of expression systems to enhance functional fidelity for translational applications.
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