| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KRT71 |
| Uniprot No | Q3SY84 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-523aa |
| 氨基酸序列 | MSRQFTCKSG AAAKGGFSGC SAVLSGGSSS SFRAGSKGLS GGFGSRSLYS LGGVRSLNVA SGSGKSGGYG FGRGRASGFA GSMFGSVALG PVCPTVCPPG GIHQVTVNES LLAPLNVELD PEIQKVRAQE REQIKALNNK FASFIDKVRF LEQQNQVLET KWELLQQLDL NNCKNNLEPI LEGYISNLRK QLETLSGDRV RLDSELRNVR DVVEDYKKRY EEEINKRTAA ENEFVLLKKD VDAAYANKVE LQAKVESMDQ EIKFFRCLFE AEITQIQSHI SDMSVILSMD NNRNLDLDSI IDEVRTQYEE IALKSKAEAE ALYQTKFQEL QLAAGRHGDD LKNTKNEISE LTRLIQRIRS EIENVKKQAS NLETAIADAE QRGDNALKDA RAKLDELEGA LHQAKEELAR MLREYQELMS LKLALDMEIA TYRKLLESEE CRMSGEFPSP VSISIISSTS GGSVYGFRPS MVSGGYVANS SNCISGVCSV RGGEGRSRGS ANDYKDTLGK GSSLSAPSKK TSR |
| 预测分子量 | 57,2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于KRT71重组蛋白的参考文献示例(注：以下内容为模拟生成，非真实文献)：
1. **文献名称**：*Recombinant Expression and Functional Analysis of KRT71 in Hair Follicle Morphogenesis*
**作者**：Smith J, et al.
**摘要**：本研究通过大肠杆菌系统成功表达重组KRT71蛋白，并验证其在毛囊形态发生中的关键作用，发现KRT71缺失导致小鼠模型毛发结构异常。
2. **文献名称**：*Structural Characterization of Recombinant Human Keratin 71 Using Cryo-EM*
**作者**：Li X, et al.
**摘要**：利用冷冻电镜技术解析重组人源KRT71蛋白的三维结构，揭示了其与角蛋白家族其他成员的结构差异及潜在功能位点。
3. **文献名称**：*KRT71 Recombinant Protein-Based Therapeutic Potential in Skin Disorders*
**作者**：Garcia R, et al.
**摘要**：通过哺乳动物细胞系表达重组KRT71蛋白，评估其在修复表皮屏障功能中的应用，为治疗遗传性皮肤疾病提供实验依据。
4. **文献名称**：*High-Yield Purification of Recombinant KRT71 for Biochemical Assays*
**作者**：Wang T, et al.
**摘要**：开发了一种高效纯化重组KRT71蛋白的层析技术，并证明其适用于体外磷酸化及蛋白相互作用研究。
建议通过PubMed或Web of Science以“KRT71 recombinant protein”为关键词检索最新文献以获取真实数据。
**Background of KRT71 Recombinant Protein**
KRT71. a member of the keratin family, encodes the keratin 71 protein, which is primarily expressed in hair follicles and epithelial tissues. This intermediate filament protein plays a critical role in maintaining structural integrity and mechanical resilience of hair shafts and skin. It is essential for proper keratinocyte differentiation and the formation of the hair cortex, influencing hair texture, curliness, and growth patterns.
Recombinant KRT71 protein is engineered in vitro using expression systems like *E. coli* or mammalian cells to produce a purified, functional form of the protein. This allows researchers to study its biochemical properties, interactions with other keratins (e.g., KRT31. KRT34), and regulatory mechanisms in hair follicle morphogenesis. Studies in animal models, including mice and dogs, have linked KRT71 mutations to hair abnormalities, such as altered coat texture or alopecia, highlighting its evolutionary conservation and biological significance.
Structurally, KRT71 contains a central α-helical rod domain flanked by variable head and tail regions, which mediate filament assembly and post-translational modifications. Dysregulation of KRT71 is implicated in ectodermal dysplasias and scarring alopecias, making it a potential therapeutic target. Recombinant KRT71 serves as a tool for investigating hair disorders, testing drug candidates, and developing gene therapies. Its role in cytoskeletal dynamics also extends to wound healing and epithelial barrier function studies.
Overall, KRT71 recombinant protein bridges basic research on keratin biology and translational applications in dermatology and regenerative medicine.
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