| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Hpt |
| Uniprot No | P00738 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 19-406aa |
| 氨基酸序列 | VDSGNDVTDIADDGCPKPPEIAHGYVEHSVRYQCKNYYKLRTEGDGVYTLNDKKQWINKAVGDKLPECEADDGCPKPPEIAHGYVEHSVRYQCKNYYKLRTEGDGVYTLNNEKQWINKAVGDKLPECEAVCGKPKNPANPVQRILGGHLDAKGSFPWQAKMVSHHNLTTGATLINEQWLLTTAKNLFLNHSENATAKDIAPTLTLYVGKKQLVEIEKVVLHPNYSQVDIGLIKLKQKVSVNERVMPICLPSKDYAEVGRVGYVSGWGRNANFKFTDHLKYVMLPVADQDQCIRHYEGSTVPEKKTPKSPVGVQPILNEHTFCAGMSKYQEDTCYGDAGSAFAVHDLEEDTWYATGILSFDKSCAVAEYGVYVKVTSIQDWVQKTIAEN |
| 预测分子量 | 57.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HPT重组蛋白的3篇参考文献示例(注：文献信息为模拟概括，具体内容请根据实际研究调整)：
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1. **文献名称**: *Expression and purification of recombinant hypoxanthine phosphoribosyltransferase (HPRT) for enzyme activity studies*
**作者**: Smith A, Johnson B
**摘要**: 研究通过大肠杆菌表达系统高效表达HPRT重组蛋白，并优化纯化流程。实验验证了重组酶的催化活性，为遗传性疾病(如Lesch-Nyhan综合征)的体外药物筛选提供了工具。
2. **文献名称**: *Recombinant haptoglobin as a therapeutic agent for hemolytic anemia*
**作者**: Boretti FS et al.
**摘要**: 开发重组结合珠蛋白(Hp)用于中和游离血红蛋白，改善溶血性贫血模型中的肾脏损伤。研究通过哺乳动物细胞表达系统获得高纯度Hp，并在动物实验中证实其生物活性。
3. **文献名称**: *Structural characterization of Helicobacter pylori Hpt protein and its role in nucleotide synthesis*
**作者**: Chen L, Wang Y
**摘要**: 解析幽门螺杆菌Hpt重组蛋白的晶体结构，揭示其参与核苷酸代谢的分子机制。通过基因敲除实验证明Hpt在细菌生长中的必要性，为抗菌靶点开发提供依据。
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**说明**：
- HPT在不同领域可能指代不同蛋白(如HPRT、Haptoglobin或病原体相关蛋白)，需结合具体研究背景选择文献。
- 建议通过PubMed或Google Scholar以“HPT recombinant protein”+研究领域(如“enzyme therapy”/“bacterial pathogenesis”)进一步筛选。
**Background of Recombinant Hpt Protein**
Recombinant Hpt (hypoxanthine phosphoribosyltransferase) protein is a key enzyme in the purine salvage pathway, enabling the recycling of purine bases for nucleotide synthesis. Its significance stems from its role in cellular metabolism and its association with genetic disorders like Lesch-Nyhan syndrome, caused by HPRT1 gene mutations. Historically, studies on Hpt have advanced understanding of nucleotide metabolism, gene regulation, and neurological dysfunction.
The development of recombinant Hpt protein leverages genetic engineering to produce the enzyme in heterologous systems, such as *E. coli*, yeast, or mammalian cells. This approach ensures high purity and scalability, addressing challenges in isolating the native protein from biological tissues. Recombinant Hpt is typically engineered with affinity tags (e.g., His-tag) for simplified purification via chromatography.
Beyond basic research, recombinant Hpt serves as a tool in drug discovery, particularly for screening therapeutic compounds targeting purine metabolism. It also aids in structural studies to elucidate enzyme mechanisms and mutation-induced dysfunction. Additionally, recombinant Hpt has applications in diagnostic assays for detecting enzyme deficiencies linked to metabolic disorders.
Recent advancements in protein engineering, such as codon optimization and fusion tags, have improved expression yields and stability. These innovations align with the growing demand for recombinant proteins in precision medicine and biomanufacturing. Overall, recombinant Hpt exemplifies how biotechnology bridges fundamental biochemistry with therapeutic and diagnostic solutions, underscoring its relevance in both academic and industrial settings.
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