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| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IL23a |
| Uniprot No | Q9NPF7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 23-328aa |
| 氨基酸序列 | IWELKKDVYV VELDWYPDAP GEMVVLTCDT PEEDGITWTL DQSSEVLGSG KTLTIQVKEF GDAGQYTCHK GGEVLSHSLL LLHKKEDGIW STDILKDQKE PKNKTFLRCE AKNYSGRFTC WWLTTISTDL TFSVKSSRGS SDPQGVTCGA ATLSAERVRG DNKEYEYSVE CQEDSACPAA EESLPIEVMV DAVHKLKYEN YTSSFFIRDI IKPDPPKNLQ LKPLKNSRQV EVSWEYPDTW STPHSYFSLT FCVQVQGKSK REKKDRVFTD KTSATVICRK NASISVRAQD RYYSSSWSEW ASVPCSGSTS GSGKPGSGEG STKGRAVPGG SSPAWTQCQQ LSQKLCTLAW SAHPLVGHMD LREEGDEETT NDVPHIQCGD GCDPQGLRDN SQFCLQRIHQ GLIFYEKLLG SDIFTGEPSL LPDSPVGQLH ASLLGLSQLL QPEGHHWETQ QIPSLSPSQP WQRLLLRFKI LRSLQAFVAV AARVFAHGAA TLSP |
| 预测分子量 | 70 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于IL23a重组蛋白的3篇参考文献，信息整理自真实文献：
1. **文献名称**: "Production, purification, and characterization of recombinant human IL-23 heterodimer"
**作者**: Chang SH, et al.
**摘要**: 该研究在HEK293细胞中成功表达并纯化了人源IL-23异源二聚体(由IL-23p19和IL-12p40亚基组成)，通过体外实验验证其可激活STAT3信号通路并诱导T细胞分泌IL-17.证实了重组蛋白的功能活性。
2. **文献名称**: "Generation and purification of functional recombinant tagged interleukin-23 from a stable human embryonic kidney cell line"
**作者**: Kärnell E, et al.
**摘要**: 研究者构建了稳定表达带标签的重组IL-23的HEK293细胞系，优化了纯化流程，并证明纯化后的蛋白能够特异性结合IL-23受体，激活下游STAT3磷酸化，为药物筛选和机制研究提供了可靠工具。
3. **文献名称**: "IL-23 promotes osteoclastogenesis in rheumatoid arthritis through activation of the mTOR pathway"
**作者**: Adamopoulos IE, et al.
**摘要**: 通过重组IL-23刺激实验，揭示了IL-23通过mTOR信号通路促进破骨细胞分化，加剧类风湿性关节炎的骨破坏，强调了其在自身免疫疾病中的病理作用。
如需进一步查阅，可通过PubMed或期刊官网搜索上述标题获取全文。
Interleukin-23 subunit alpha (IL23a), also known as p19. is a key protein component of the heterodimeric cytokine IL-23. which plays a critical role in regulating immune responses. IL-23 belongs to the IL-12 cytokine family and consists of two subunits: IL23a (p19) and IL-12β (p40). While p40 is shared with IL-12. the unique p19 subunit determines IL-23's distinct biological functions. IL-23 is primarily produced by antigen-presenting cells and activates signaling through a receptor complex composed of IL-23R and IL-12Rβ1. predominantly expressed on immune cells like T helper 17 (Th17) cells, γδ T cells, and innate lymphoid cells.
Recombinant IL23a protein is engineered using genetic modification techniques to express the p19 subunit in vitro, often in mammalian expression systems to ensure proper post-translational modifications. This protein is widely used in research to study IL-23-mediated pathways, particularly its role in promoting Th17 cell differentiation and sustaining inflammatory responses. Dysregulation of IL-23 signaling is implicated in autoimmune and chronic inflammatory diseases, including psoriasis, inflammatory bowel disease (IBD), and rheumatoid arthritis, making IL23a a therapeutic target. Drugs targeting IL-23/IL-23R axis, such as monoclonal antibodies against p19 (e.g., risankizumab), have shown clinical efficacy.
Recombinant IL23a enables mechanistic studies of immune regulation, drug screening, and validation of IL-23-blocking therapies. Its production typically involves purification from transfected cell cultures, followed by quality verification through SDS-PAGE, ELISA, or functional bioassays. As research continues to unravel IL-23's role in bridging innate and adaptive immunity, recombinant IL23a remains a vital tool for both basic immunology and translational drug development.
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