| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CK15 |
| Uniprot No | P19012 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-456aa |
| 氨基酸序列 | MTTTFLQTSS STFGGGSTRG GSLLAGGGGF GGGSLSGGGG SRSISASSAR FVSSGSGGGY GGGMRVCGFG GGAGSVFGGG FGGGVGGGFG GGFGGGDGGL LSGNEKITMQ NLNDRLASYL DKVRALEEAN ADLEVKIHDW YQKQTPTSPE CDYSQYFKTI EELRDKIMAT TIDNSRVILE IDNARLAADD FRLKYENELA LRQGVEADIN GLRRVLDELT LARTDLEMQI EGLNEELAYL KKNHEEEMKE FSSQLAGQVN VEMDAAPGVD LTRVLAEMRE QYEAMAEKNR RDVEAWFFSK TEELNKEVAS NTEMIQTSKT EITDLRRTMQ ELEIELQSQL SMKAGLENSL AETECRYATQ LQQIQGLIGG LEAQLSELRC EMEAQNQEYK MLLDIKTRLE QEIATYRSLL EGQDAKMAGI AIREASSGGG GSSSNFHINV EESVDGQVVS SHKREI |
| 预测分子量 | 49,2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CK15重组蛋白的3篇参考文献示例，包括文献名称、作者及摘要内容概述：
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1. **文献名称**: *"Recombinant Keratin 15 Expression in Epithelial Stem Cells: Cloning and Functional Analysis"*
**作者**: Smith A, et al.
**摘要**: 本研究成功在大肠杆菌系统中重组表达了人源CK15蛋白，并验证其作为表皮干细胞标志物的功能。通过体外实验证实重组CK15在维持干细胞自我更新及分化调控中的关键作用。
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2. **文献名称**: *"Engineering Recombinant Keratin 15 for Biomaterial Applications in Tissue Regeneration"*
**作者**: Lee J, Kim H.
**摘要**: 报道利用哺乳动物细胞表达系统生产高纯度重组CK15.并开发基于CK15的生物支架材料。实验表明该材料可显著促进皮肤创面修复和毛囊再生。
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3. **文献名称**: *"Structural Characterization of Recombinant CK15 and Its Role in Tumor Progression"*
**作者**: Chen R, et al.
**摘要**: 通过X射线晶体学解析重组CK15蛋白的三维结构，结合功能实验揭示其在肿瘤干细胞侵袭转移中的调控机制，为靶向治疗提供新思路。
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以上文献均为示例，实际引用时需以具体发表的论文为准。
CK15. or cytokeratin 15. is a member of the cytokeratin family of intermediate filament proteins, which are critical structural components of epithelial cells. These proteins form a network that maintains cellular integrity, resists mechanical stress, and facilitates cell signaling. CK15 is specifically expressed in stem cell-rich regions of epithelial tissues, such as the basal layer of the epidermis and hair follicles, where it serves as a marker for progenitor cells. Its expression is often associated with regenerative capacity and tissue homeostasis, making it a focus in studies on wound healing, epithelial differentiation, and cancer biology.
Recombinant CK15 protein is produced using biotechnological methods, typically through expression in bacterial, insect, or mammalian cell systems. The choice of expression system depends on the need for post-translational modifications, such as phosphorylation or glycosylation, which can influence protein function. Recombinant CK15 retains the structural and functional properties of its native counterpart, enabling researchers to study its role in cellular processes in vitro or in animal models. Purification techniques, such as affinity chromatography, ensure high purity and stability for experimental use.
In research, recombinant CK15 is employed to investigate epithelial stem cell dynamics, tumor initiation, and metastasis, as its dysregulation is linked to cancers like basal cell carcinoma and triple-negative breast cancer. It also aids in developing tissue engineering strategies for skin regeneration. Challenges in working with recombinant CK15 include maintaining solubility due to its fibrous nature and ensuring proper folding in heterologous expression systems. Despite these hurdles, its applications in basic and translational research underscore its importance in understanding epithelial biology and disease mechanisms.
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