| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NFKB3 |
| Uniprot No | Q04206 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-551aa |
| 氨基酸序列 | MDELFPLIFPAEPAQASGPYVEIIEQPKQRGMRFRYKCEGRSAGSIPGERSTDTTKTHPTIKINGYTGPGTVRISLVTKDPPHRPHPHELVGKDCRDGFYEAELCPDRCIHSFQNLGIQCVKKRDLEQAISQRIQTNNNPFQVPIEEQRGDYDLNAVRLCFQVTVRDPSGRPLRLPPVLSHPIFDNRAPNTAELKICRVNRNSGSCLGGDEIFLLCDKVQKEDIEVYFTGPGWEARGSFSQADVHRQVAIVFRTPPYADPSLQAPVRVSMQLRRPSDRELSEPMEFQYLPDTDDRHRIEEKRKRTYETFKSIMKKSPFSGPTDPRPPPRRIAVPSRSSASVPKPAPQPYPFTSSLSTINYDEFPTMVFPSGQISQASALAPAPPQVLPQAPAPAPAPAMVSALAQAPAPVPVLAPGPPQAVAPPAPKPTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNSEFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGDEDFSSIADMDFSALLSQISS |
| 预测分子量 | 60,2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是模拟生成的关于NF-κB p65(常被误称为NFKB3)重组蛋白的参考文献示例。请注意，这些文献信息为虚构，仅用于演示格式：
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1. **标题**：*"Recombinant NF-κB p65 Expression in E. coli and DNA-Binding Activity Analysis"*
**作者**：Smith J, Lee K, et al.
**摘要**：本研究描述了一种在大肠杆菌中高效表达重组NF-κB p65蛋白的方法，通过亲和层析纯化并验证其与靶DNA序列的结合能力，为体外研究NF-κB信号通路提供工具。
2. **标题**：*"Structural Insights into NF-κB p65 Phosphorylation via Recombinant Protein Crystallography"*
**作者**：Chen L, Wang H, et al.
**摘要**：利用重组p65蛋白解析其磷酸化位点的晶体结构，揭示IKK激酶对其活化的分子机制，为设计抗炎药物提供结构基础。
3. **标题**：*"Functional Screening of NF-κB Inhibitors Using Recombinant p65/RelA Protein"*
**作者**：Rodriguez M, Gupta S, et al.
**摘要**：开发基于重组p65蛋白的高通量筛选平台，鉴定出多种小分子化合物可阻断p65与DNA的相互作用，具有潜在抗肿瘤应用价值。
4. **标题**：*"Recombinant p65-Mediated Transcriptional Activation in Inflammatory Response Models"*
**作者**：Kim T, Park S, et al.
**摘要**：通过体外重组p65蛋白与炎症相关基因启动子的结合实验，阐明其在TNF-α诱导的炎症反应中的调控作用。
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**注意**：以上文献为示例性质，实际研究中请通过PubMed、Web of Science等数据库检索真实发表的论文。若需具体领域文献，建议补充关键词(如“重组表达”、“结构功能”等)。
**Background of NFKB3 Recombinant Protein**
NFKB3 (Nuclear Factor Kappa B Subunit 3), also known as p52. is a member of the NF-κB transcription factor family, which regulates genes involved in immune response, inflammation, cell survival, and proliferation. It is encoded by the *NFKB2* gene and is synthesized as a precursor protein, p100. which undergoes proteasomal processing to generate the mature p52 subunit. This processing is tightly regulated by the non-canonical NF-κB pathway, often activated by cytokines like CD40 ligand, BAFF, or lymphotoxin-β.
The recombinant NFKB3/p52 protein is produced using biotechnological methods, such as expression in *E. coli* or mammalian cell systems, followed by purification to ensure high stability and activity. Recombinant p52 typically retains the ability to dimerize with other NF-κB subunits (e.g., RelB) and bind DNA at κB motifs to modulate target gene expression.
Functionally, p52 plays a critical role in lymphoid organ development, B-cell maturation, and adaptive immunity. Dysregulation of NFKB3 is linked to autoimmune diseases, chronic inflammation, and cancers, particularly those involving aberrant lymphoid signaling. Recombinant p52 is widely used in research to study NF-κB signaling mechanisms, screen inhibitors, or explore its interactions with other proteins or DNA. Its application extends to *in vitro* assays, such as EMSA (electrophoretic mobility shift assays), luciferase reporter systems, and structural studies to resolve molecular interactions.
Overall, NFKB3 recombinant protein serves as a vital tool for dissecting the non-canonical NF-κB pathway and developing therapeutic strategies targeting its dysregulation.
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