| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CK9 |
| Uniprot No | Q9NQ34 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-198aa |
| 氨基酸序列 | MATLWGGLLRLGSLLSLSCLALSVLLLAQLSDAAKNFEDVRCKCICPPYKENSGHIYNKNISQKDCDCLHVVEPMPVRGPDVEAYCLRCECKYEERSSVTIKVTIIIYLSILGLLLLYMVYLTLVEPILKRRLFGHAQLIQSDDDIGDHQPFANAHDVLARSRSRANVLNKVEYAQQRWKLQVQEQRKSVFDRHVVLS |
| 预测分子量 | 22,5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CK9重组蛋白的模拟参考文献示例(注：CK9重组蛋白相关研究较少，以下内容为假设性示例，建议根据实际文献调整)：
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1. **标题**: "Expression and Purification of Recombinant CK9 Protein in E. coli"
**作者**: Zhang L, et al.
**摘要**: 研究报道了利用大肠杆菌系统表达CK9重组蛋白，通过His标签亲和层析纯化，并验证了其分子量及体外激酶活性，为功能研究提供材料基础。
2. **标题**: "Functional Characterization of CK9 in Cell Cycle Regulation"
**作者**: Smith J, Patel R.
**摘要**: 通过体外实验发现，重组CK9蛋白可磷酸化特定细胞周期相关蛋白，提示其在有丝分裂调控中的潜在作用。
3. **标题**: "CK9 Recombinant Protein as a Diagnostic Marker for Liver Cancer"
**作者**: Kim H, et al.
**摘要**: 研究将CK9重组蛋白作为抗原制备单克隆抗体，发现其在肝癌患者血清中高表达，可能成为新型生物标志物。
4. **标题**: "Structural Analysis of CK9 Using Cryo-EM"
**作者**: Wang X, et al.
**摘要**: 利用冷冻电镜解析了重组CK9蛋白的三维结构，揭示了其催化活性位点的构象特征，为靶向药物设计提供依据。
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**注意**：以上内容为模拟示例，实际文献需通过PubMed、Web of Science等平台检索确认。若CK9为笔误(如应为CK1ε、CK2等)，建议核实后重新检索。
CK9 recombinant protein refers to a genetically engineered form of Cytokeratin 9. a member of the cytokeratin family that constitutes intermediate filaments in epithelial cells. Cytokeratins are structural proteins critical for maintaining cellular integrity, mechanical stability, and stress resistance. CK9 is specifically expressed in the suprabasal layers of the epidermis, particularly in palmoplantar (palm and sole) skin, where it plays a key role in reinforcing the structural framework of keratinocytes. Mutations in the KRT9 gene encoding CK9 are linked to epidermolytic palmoplantar keratoderma (EPPK), a rare hereditary skin disorder characterized by hyperkeratosis and blistering.
The recombinant CK9 protein is typically produced in heterologous expression systems, such as E. coli or mammalian cells, to ensure high purity and functionality. Its production enables detailed study of CK9’s molecular interactions, post-translational modifications, and role in disease mechanisms. Researchers utilize CK9 recombinant protein to investigate its binding partners, filament assembly dynamics, and responses to mechanical stress. It also serves as a critical tool for developing therapeutic strategies, including drug screening for EPPK and gene therapy approaches. Additionally, CK9 recombinant protein is employed in antibody production, diagnostic assays, and as a reference standard in biomarker studies. Its availability accelerates research into epithelial biology, dermatological disorders, and the broader field of intermediate filament pathology.
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