| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CK14 |
| Uniprot No | P02533 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-472aa |
| 氨基酸序列 | MTTCSRQFTSSSSMKGSCGIGGGIGGGSSRISSVLAGGSCRAPSTYGGGLSVSSSRFSSGGACGLGGGYGGGFSSSSSSFGSGFGGGYGGGLGAGLGGGFGGGFAGGDGLLVGSEKVTMQNLNDRLASYLDKVRALEEANADLEVKIRDWYQRQRPAEIKDYSPYFKTIEDLRNKILTATVDNANVLLQIDNARLAADDFRTKYETELNLRMSVEADINGLRRVLDELTLARADLEMQIESLKEELAYLKKNHEEEMNALRGQVGGDVNVEMDAAPGVDLSRILNEMRDQYEKMAEKNRKDAEEWFFTKTEELNREVATNSELVQSGKSEISELRRTMQNLEIELQSQLSMKASLENSLEETKGRYCMQLAQIQEMIGSVEEQLAQLRCEMEQQNQEYKILLDVKTRLEQEIATYRRLLEGEDAHLSSSQFSSGSQSSRDVTSSSRQIRTKVMDVHDGKVVSTHEQVLRTKN |
| 预测分子量 | 53.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CK14(Cytokeratin 14)重组蛋白的3篇代表性文献示例：
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1. **文献名称**：*"Recombinant Keratin 14 Production and Its Role in Epithelial Cancer Metastasis"*
**作者**：Smith A, et al.
**摘要**：研究通过大肠杆菌表达系统重组生产CK14蛋白，并探讨其在乳腺癌细胞侵袭转移中的作用，发现CK14过表达促进肿瘤细胞迁移和基质侵袭。
2. **文献名称**：*"Expression and Purification of Functional Human Cytokeratin 14 in a Baculovirus System"*
**作者**：Li H, et al.
**摘要**：利用杆状病毒-昆虫细胞系统高效表达CK14重组蛋白，优化纯化流程，验证其与胞内蛋白相互作用的能力，为体外研究中间丝组装提供工具。
3. **文献名称**：*"Monoclonal Antibody Generation Against Recombinant CK14 for Diagnostic Applications"*
**作者**：Wang X, et al.
**摘要**：通过原核表达CK14重组蛋白免疫小鼠，开发高特异性单克隆抗体，成功应用于皮肤基底细胞癌的免疫组化检测，提升病理诊断准确性。
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注：以上文献信息为示例，实际引用时需根据具体研究查阅PubMed或Google Scholar获取真实文献。
CK14 (Cytokeratin 14) is a type I intermediate filament protein encoded by the *KRT14* gene in humans. It is primarily expressed in the basal layer of stratified epithelia, including skin, hair follicles, and mucosal tissues, where it pairs with its type II partner, CK5. to form a heterodimeric structural scaffold. This CK5/CK14 network provides mechanical resilience to epithelial cells, maintaining tissue integrity under physical stress. CK14 is crucial for cell adhesion, migration, and signaling, and its expression is tightly regulated during epithelial differentiation and wound healing.
Recombinant CK14 protein is engineered using expression systems like *E. coli* or mammalian cells, enabling controlled production of the protein for research and therapeutic applications. Its recombinant form retains the native structure and biochemical properties, facilitating studies on epithelial biology, cancer progression, and genetic disorders. Mutations in *KRT14* are linked to epidermolysis bullosa simplex (EBS), a blistering skin disorder, making recombinant CK14 a valuable tool for modeling disease mechanisms and testing gene therapies.
In cancer research, CK14 is a biomarker for aggressive subtypes, such as basal-like breast cancer and squamous cell carcinomas. Recombinant CK14 aids in investigating tumor invasion, metastasis, and drug resistance mechanisms. Additionally, it supports regenerative medicine efforts, including tissue engineering of skin equivalents. The protein’s role in stem cell maintenance further underscores its relevance in developmental biology.
Despite its utility, producing functional recombinant CK14 requires optimizing post-translational modifications (e.g., phosphorylation) to mimic native behavior. Advances in protein engineering and 3D cell culture systems continue to enhance its applications, bridging gaps between basic research and clinical translation.
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