| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GSK3a |
| Uniprot No | P49840 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-483aa |
| 氨基酸序列 | SGGGPSGGG PGGSGRARTS SFAEPGGGGG GGGGGPGGSA SGPGGTGGGK ASVGAMGGGV GASSSGGGPG GSGGGGSGGP GAGTSFPPPG VKLGRDSGKV TTVVATLGQG PERSQEVAYT DIKVIGNGSF GVVYQARLAE TRELVAIKKV LQDKRFKNRE LQIMRKLDHC NIVRLRYFFY SSGEKKDELY LNLVLEYVPE TVYRVARHFT KAKLTIPILY VKVYMYQLFR SLAYIHSQGV CHRDIKPQNL LVDPDTAVLK LCDFGSAKQL VRGEPNVSYI CSRYYRAPEL IFGATDYTSS IDVWSAGCVL AELLLGQPIF PGDSGVDQLV EIIKVLGTPT REQIREMNPN YTEFKFPQIK AHPWTKVFKS RTPPEAIALC SSLLEYTPSS RLSPLEACAH SFFDELRCLG TQLPNNRPLP PLFNFSAGEL SIQPSLNAIL IPPHLRSPAG TTTLTPSSQA LTETPTSSDW QSTDATPTLT NSS |
| 预测分子量 | 51 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GSK3α重组蛋白的3篇参考文献的简要信息，涵盖其表达、功能及结构研究：
---
1. **文献名称**: "Expression and purification of active human GSK-3α in Escherichia coli for structural studies"
**作者**: Patel, S. et al.
**摘要**: 本研究利用大肠杆菌系统重组表达人源GSK3α蛋白，优化了纯化条件并获得高纯度蛋白。通过体外激酶实验验证其磷酸化底物(如β-catenin)的活性，并利用X射线晶体学解析了其催化结构域的晶体结构，揭示了ATP结合口袋的关键残基。
---
2. **文献名称**: "Functional characterization of recombinant GSK3α in Alzheimer’s disease models"
**作者**: Hernandez, F. et al.
**摘要**: 研究通过昆虫细胞(Sf9)系统表达重组GSK3α，探究其在阿尔茨海默病中的作用。结果显示，GSK3α磷酸化Tau蛋白的能力强于GSK3β，且与Aβ肽协同加剧神经毒性，提示其作为治疗靶点的潜力。
---
3. **文献名称**: "Development of a high-throughput screening assay for GSK3α inhibitors using recombinant protein"
**作者**: Zhang, L. et al.
**摘要**: 构建基于荧光共振能量转移(FRET)的GSK3α激酶活性检测体系，利用重组人GSK3α蛋白筛选小分子抑制剂。鉴定出多个选择性抑制GSK3α(而非GSK3β)的化合物，为糖尿病和癌症药物开发提供新候选分子。
---
**备注**：以上文献信息为示例性内容，实际引用时需核实具体文献来源及准确性。如需真实文献推荐，建议通过PubMed或Web of Science以“GSK3α recombinant protein”为关键词检索近年研究。
Glycogen synthase kinase 3 alpha (GSK3α) is a serine/threonine kinase belonging to the GSK3 family, alongside its isoform GSK3β. Initially identified for its role in glycogen metabolism by phosphorylating glycogen synthase, GSK3α is now recognized as a multifunctional regulator involved in diverse cellular processes, including cell proliferation, differentiation, apoptosis, and embryonic development. Unlike GSK3β, which has been extensively studied, GSK3α shares structural homology but exhibits distinct substrate preferences and regulatory mechanisms, partly due to differences in its N-terminal region. Both isoforms are constitutively active under basal conditions and are inhibited by phosphorylation at specific residues (e.g., Ser21 in GSK3α) in response to signaling pathways such as insulin/Akt and Wnt/β-catenin.
Recombinant GSK3α protein is engineered for research applications to study its enzymatic activity, substrate interactions, and regulatory networks. Produced using heterologous expression systems (e.g., E. coli, mammalian cells), the recombinant protein retains kinase activity and post-translational modification sites, enabling in vitro assays and drug screening. It is widely used to investigate GSK3α's role in diseases such as Alzheimer's disease, cancer, and diabetes, where dysregulated GSK3α contributes to pathological mechanisms like tau hyperphosphorylation or insulin resistance. Additionally, recombinant GSK3α serves as a tool for developing isoform-specific inhibitors, addressing the challenge of distinguishing between GSK3α and GSK3β in therapeutic targeting. Its availability accelerates mechanistic studies and translational research, bridging gaps in understanding isoform-specific functions and therapeutic potential.
×
