| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CEBPa |
| Uniprot No | P49715 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-358aa |
| 氨基酸序列 | MESADFYEAE PRPPMSSHLQ SPPHAPSSAA FGFPRGAGPA QPPAPPAAPE PLGGICEHET SIDISAYIDP AAFNDEFLAD LFQHSRQQEK AKAAVGPTGG GGGGDFDYPG APAGPGGAVM PGGAHGPPPG YGCAAAGYLD GRLEPLYERV GAPALRPLVI KQEPREEDEA KQLALAGLFP YQPPPPPPPS HPHPHPPPAH LAAPHLQFQI AHCGQTTMHL QPGHPTPPPT PVPSPHPAPA LGAAGLPGPG SALKGLGAAH PDLRASGGSG AGKAKKSVDK NSNEYRVRRE RNNIAVRKSR DKAKQRNVET QQKVLELTSD NDRLRKRVEQ LSRELDTLRG IFRQLPESSL VKAMGNCA |
| 预测分子量 | 37,5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CEBPa(CEBPα)重组蛋白的3-4篇示例参考文献(注：以下内容为模拟示例，实际文献需通过学术数据库查询确认)：
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1. **标题**：*"High-level expression and purification of recombinant human C/EBPα in Escherichia coli for functional studies"*
**作者**：Smith J, Brown K, Lee M.
**摘要**：研究报道了在大肠杆菌中高效表达并纯化人源重组CEBPα蛋白的方法，通过优化密码子和纯化步骤获得高活性蛋白，用于体外DNA结合和转录调控分析。
2. **标题**：*"Crystal structure of C/EBPα bZIP domain reveals dimerization and DNA-binding mechanisms"*
**作者**：Johnson R, Zhang Y, Wang L.
**摘要**：通过X射线晶体学解析了CEBPα的bZIP功能域结构，阐明了其二聚化及与DNA结合的分子机制，为设计靶向CEBPα的调控分子提供结构基础。
3. **标题**：*"Recombinant C/EBPα induces granulocytic differentiation in acute myeloid leukemia cell lines"*
**作者**：Chen H, Müller A, Tenen DG.
**摘要**：研究发现外源性重组CEBPα蛋白可激活白血病细胞的分化通路，抑制增殖并诱导粒细胞分化，提示其潜在治疗应用。
4. **标题**：*"CEBPα recombinant protein regulates lipid metabolism in adipocytes via PPARγ interaction"*
**作者**：Wang X, Tang Q, Lazar MA.
**摘要**：探讨重组CEBPα在脂肪细胞分化中的作用，证明其通过调控PPARγ等靶基因影响脂代谢，揭示其在代谢疾病中的功能。
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如需具体文献，建议在PubMed、Web of Science等平台以关键词**"CEBPa recombinant protein"**或**"CEBPα purification/function"**检索。
CEBPA (CCAAT/enhancer-binding protein alpha) is a critical transcription factor involved in the regulation of cellular differentiation, metabolism, and immune responses. It belongs to the C/EBP family of proteins, which share a basic leucine zipper (bZIP) domain for DNA binding and dimerization. CEBPA plays a pivotal role in hematopoiesis, particularly in granulocyte maturation, and is essential for adipogenesis and liver function. Its expression is tightly regulated, and disruptions are linked to diseases such as acute myeloid leukemia (AML), where CEBPA mutations are associated with poor prognosis.
Recombinant CEBPA protein is engineered using biotechnological methods, often expressed in bacterial (e.g., E. coli) or mammalian systems to ensure proper folding and post-translational modifications. This protein retains the functional domains of native CEBPA, including the transactivation domains and bZIP region, enabling studies on DNA binding, protein interactions, and transcriptional regulation. Researchers use it to investigate mechanisms of cell differentiation, metabolic disorders, and leukemogenesis. In therapeutic contexts, recombinant CEBPA has been explored to restore normal differentiation in AML cells or modulate metabolic pathways in obesity-related conditions. Its production and characterization also support drug discovery efforts targeting CEBPA-associated pathways. Overall, recombinant CEBPA serves as a vital tool for dissecting its biological roles and developing potential therapies for related diseases.
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