| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CD56 |
| Uniprot No | P13591 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 31-677aa |
| 氨基酸序列 | ISVGESKFFLCQVAGDAKDKDISWFSPNGEKLTPNQQRISVVWNDDSSSTLTIYNANIDDAGIYKCVVTGEDGSESEATVNVKIFQKLMFKNAPTPQEFREGEDAVIVCDVVSSLPPTIIWKHKGRDVILKKDVRFIVLSNNYLQIRGIKKTDEGTYRCEGRILARGEINFKDIQVIVNVPPTIQARQNIVNATANLGQSVTLVCDAEGFPEPTMSWTKDGEQIEQEEDDEKYIFSDDSSQLTIKKVDKNDEAEYICIAENKAGEQDATIHLKVFAKPKITYVENQTAMELEEQVTLTCEASGDPIPSITWRTSTRNISSEEKTLDGHMVVRSHARVSSLTLKSIQYTDAGEYICTASNTIGQDSQSMYLEVQYAPKLQGPVAVYTWEGNQVNITCEVFAYPSATISWFRDGQLLPSSNYSNIKIYNTPSASYLEVTPDSENDFGNYNCTAVNRIGQESLEFILVQADTPSSPSIDQVEPYSSTAQVQFDEPEATGGVPILKYKAEWRAVGEEVWHSKWYDAKEASMEGIVTIVGLKPETTYAVRLAALNGKGLGEISAASEFKTQPVQGEPSAPKLEGQMGEDGNSIKVNLIKQDDGGSPIRHYLVRYRALSSEWKPEIRLPSGSDHVMLKSLDWNAEYEVYVVAE |
| 预测分子量 | 75.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CD56重组蛋白的3篇代表性文献及其摘要概括：
1. **"Expression and Functional Characterization of Recombinant CD56 in Natural Killer Cells"**
*作者：Smith A, et al.*
摘要：该研究通过哺乳动物表达系统成功制备了重组CD56蛋白，并验证其在NK细胞中的功能，证明CD56通过介导细胞间黏附增强NK细胞对肿瘤靶细胞的杀伤活性。
2. **"Structural Insights into the CD56 Extracellular Domain Using Recombinant Protein Crystallography"**
*作者：Zhang L, et al.*
摘要：通过重组表达和纯化CD56胞外段蛋白，结合X射线晶体学解析其三维结构，揭示了CD56与配体结合的分子机制，为靶向药物设计提供结构基础。
3. **"Recombinant CD56 as a Biomarker for Neuroendocrine Tumors: Production and Clinical Validation"**
*作者：Kim H, et al.*
摘要：开发了一种高纯度重组CD56蛋白，并用于建立ELISA检测方法，证明其在神经内分泌肿瘤患者血清中的诊断价值，显著提高检测灵敏度和特异性。
4. **"Engineering Soluble CD56 Fusion Proteins for Immunotherapy Applications"**
*作者：Wang Y, et al.*
摘要：利用基因工程技术构建可溶性CD56-Fc融合蛋白，评估其在体外和动物模型中的免疫调节功能，显示其潜在应用于癌症免疫治疗的可行性。
(注：以上文献信息为示例性内容，实际引用需以真实文献为准。)
CD56. also known as neural cell adhesion molecule (NCAM), is a transmembrane glycoprotein belonging to the immunoglobulin (Ig) superfamily. It plays critical roles in cell-cell adhesion, neurite outgrowth, synaptic plasticity, and immune regulation. CD56 is encoded by the *NCAM1* gene and exists as multiple isoforms generated by alternative splicing. The protein features extracellular Ig-like domains, fibronectin type III repeats, and a polysialic acid modification that modulates its adhesive properties. Naturally expressed in neurons, natural killer (NK) cells, and certain T-cell subsets, CD56 is a biomarker for NK-cell malignancies and neuroendocrine tumors.
Recombinant CD56 protein is engineered using expression systems (e.g., mammalian, insect, or bacterial cells) to produce purified, functional forms for research and therapeutic applications. Mammalian systems (e.g., CHO or HEK293 cells) are preferred for generating glycosylated isoforms that mimic native protein activity. The recombinant protein retains key domains, enabling studies on cell adhesion mechanisms, immune cell signaling (e.g., NK cell cytotoxicity), and neural development pathways. It also serves as a critical reagent in drug discovery, particularly for cancers and neurological disorders, where CD56-mediated interactions influence disease progression. Additionally, it aids in developing diagnostic tools, such as antibody validation and biomarker assays. Its versatility in structural and functional studies underscores its importance in both basic research and clinical translation.
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