| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ITGa1 |
| Uniprot No | P56199 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 951-1059aa |
| 氨基酸序列 | QFYSSASEYHISIAANETVPEVINSTEDIGNEINIFYLIRKSGSFPMPEL KLSISFPNMTSNGYPVLYPTGLSSSENANCRPHIFEDPFSINSGKKMTTS TDHLKRGTI |
| 预测分子量 | 38 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是生成的示例参考文献，用于演示格式和内容(请注意，这些文献为虚构示例，实际引用时请查询真实数据库)：
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1. **文献名称**: "Recombinant Expression and Functional Characterization of Integrin α1 in Collagen Binding"
**作者**: Zhang L, et al.
**摘要**: 本研究报道了人源ITGa1重组蛋白在大肠杆菌中的表达与纯化，并证实其与胶原蛋白的特异性结合能力，为研究整合素α1在细胞基质黏附中的作用提供工具。
2. **文献名称**: "ITGA1 Recombinant Protein Attenuates Fibrosis in Murine Liver Injury Models"
**作者**: Smith JR, et al.
**摘要**: 通过哺乳动物细胞系统表达ITGa1重组蛋白，实验证明其可通过阻断过度胶原沉积减轻小鼠肝纤维化，提示其作为潜在治疗分子的价值。
3. **文献名称**: "Optimization of ITGa1 Purification for Structural Studies Using Cryo-EM"
**作者**: Gupta A, et al.
**摘要**: 开发了一种高效纯化方案，从昆虫细胞中获取高纯度ITGa1重组蛋白，并解析其胞外域结构，为靶向药物设计提供结构基础。
4. **文献名称**: "Role of Recombinant ITGa1 in T Cell Activation and Autoimmune Disease Pathways"
**作者**: Tanaka K, et al.
**摘要**: 利用真核表达系统制备功能性ITGa1蛋白，发现其参与调控T细胞活化和自身免疫反应，为相关疾病机制研究提供新方向。
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**注意**：以上文献为模拟示例，实际研究中请通过PubMed、Google Scholar等平台检索真实文献，并使用正确的引用格式(如APA、MLA等)。
**Background of ITGA1 Recombinant Protein**
Integrin alpha-1 (ITGA1), a subunit of the integrin family, is a cell surface receptor that mediates interactions between cells and the extracellular matrix (ECM). Integrins are heterodimeric proteins composed of α and β subunits, with ITGA1 pairing exclusively with the β1 subunit (ITGB1) to form the α1β1 integrin. This receptor primarily binds collagen and laminin, playing critical roles in cell adhesion, migration, and signaling during tissue development, homeostasis, and repair.
ITGA1 is expressed in various tissues, including fibroblasts, endothelial cells, and certain immune cells. It regulates ECM remodeling, angiogenesis, and immune responses by modulating cellular responses to mechanical and biochemical cues. Dysregulation of ITGA1 has been implicated in pathological conditions such as fibrosis, cancer progression, and inflammatory diseases, highlighting its therapeutic potential.
Recombinant ITGA1 protein is engineered *in vitro* using expression systems (e.g., mammalian, insect, or bacterial cells) to produce purified, functional α1 subunits for research applications. This protein retains key structural domains, including the ligand-binding extracellular region, transmembrane helix, and cytoplasmic tail, enabling studies on integrin-ligand interactions, signaling pathways, and cell-ECM dynamics.
In research, recombinant ITGA1 is utilized to investigate integrin-mediated cellular behaviors, screen therapeutic agents targeting integrin pathways, and develop disease models. Its availability accelerates mechanistic studies and drug discovery, offering insights into tissue engineering and precision medicine strategies targeting ECM-related disorders.
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