| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | bHB |
| Uniprot No | O14503 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-412aa |
| 氨基酸序列 | MERIPSAQPPPACLPKAPGLEHGDLPGMYPAHMYQVYKSRRGIKRSEDSKETYKLPHRLIEKKRRDRINECIAQLKDLLPEHLKLTTLGHLEKAVVLELTLKHVKALTNLIDQQQQKIIALQSGLQAGELSGRNVETGQEMFCSGFQTCAREVLQYLAKHENTRDLKSSQLVTHLHRVVSELLQGGTSRKPSDPAPKVMDFKEKPSSPAKGSEGPGKNCVPVIQRTFAHSSGEQSGSDTDTDSGYGGESEKGDLRSEQPCFKSDHGRRFTMGERIGAIKQESEEPPTKKNRMQLSDDEGHFTSSDLISSPFLGPHPHQPPFCLPFYLIPPSATAYLPMLEKCWYPTSVPVLYPGLNASAAALSSFMNPDKISAPLLMPQRLPSPLPAHPSVDSSVLLQALKPIPPLNLETKD |
| 预测分子量 | 45,5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于bHB重组蛋白的3-4条参考文献示例(内容为虚构，仅供格式参考)：
1. **文献名称**: "Efficient Expression and Purification of Recombinant bHB Protein in E. coli"
**作者**: Smith J. et al.
**摘要**: 该研究通过优化大肠杆菌表达系统，成功实现了bHB重组蛋白的高效可溶性表达，并开发了基于亲和层析的纯化工艺，为大规模生产奠定了基础。
2. **文献名称**: "Structural and Functional Characterization of bHB Recombinant Protein in Disease Models"
**作者**: Chen L. et al.
**摘要**: 通过X射线晶体学解析了bHB蛋白的三维结构，并在细胞模型中验证其抗氧化活性，表明其在神经退行性疾病治疗中的潜在应用价值。
3. **文献名称**: "Optimization of bHB Expression in Pichia pastoris for Industrial Applications"
**作者**: Wang Y. et al.
**摘要**: 利用毕赤酵母系统表达bHB重组蛋白，通过密码子优化和发酵条件调控将产量提高至5 g/L，适用于工业级酶催化反应。
4. **文献名称**: "Thermostability Enhancement of bHB Recombinant Protein via Site-Directed Mutagenesis"
**作者**: Gupta R. et al.
**摘要**: 通过理性设计突变bHB蛋白的关键氨基酸残基，显著提高了其高温环境下的稳定性，拓展了其在高温生物工艺中的应用场景。
注：以上文献为示例，实际研究中请以真实发表的论文为准。
**Background of bHB Recombinant Protein**
The bHB recombinant protein is a bioengineered molecule designed to mimic or enhance specific biological functions, typically associated with metabolic regulation or cellular signaling. The term "bHB" commonly refers to β-hydroxybutyrate (β-HB), a ketone body produced during fatty acid metabolism, which plays a critical role in energy homeostasis, particularly under low-carbohydrate conditions like fasting or ketogenic diets. Recombinant versions of proteins linked to β-HB pathways are of growing interest due to their therapeutic potential in metabolic disorders, neurodegenerative diseases, and aging-related conditions.
Recombinant protein technology enables the production of bHB-associated proteins (e.g., enzymes, receptors, or transport proteins) in heterologous systems such as *E. coli*, yeast, or mammalian cells. These systems allow for high-yield, scalable synthesis with precise post-translational modifications, ensuring functional equivalence to native proteins. For instance, recombinant enzymes involved in ketogenesis (e.g., HMG-CoA synthase/lyase) are studied for their ability to modulate β-HB levels, which may aid in treating insulin resistance or mitochondrial dysfunction.
Additionally, bHB itself has gained attention as a signaling molecule that influences histone deacetylase inhibition, inflammation reduction, and oxidative stress mitigation. Recombinant proteins targeting β-HB receptors (e.g., GPR109A) or its metabolic pathways are explored for drug development. Challenges include optimizing protein stability, delivery mechanisms, and minimizing immunogenicity.
Overall, bHB recombinant proteins represent a convergence of metabolic research and biotechnology, offering novel strategies to address diseases linked to energy dysregulation. Their development underscores the importance of ketone biology in both basic science and translational medicine.
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