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| 纯度 | >90%SDS-PAGE. |
| 种属 | mouse |
| 靶点 | THFA |
| Uniprot No | Q8VCZ9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-456aa |
| 氨基酸序列 | MIWTRLPLYG PSKPSTGGWQ PLRFDGGAFH VKGTAELARA LLVLRLCAWP PLVTHGLAFQ AWSQRLLGSR LSGALLRASI YGQFVAGETA EEVRNCVGQL QALGLQPLLA VPTEEEPDST AKTSEVWYEE NLSAMLRCVD LSRALVDAHG PARNSLMQLK VTALASTRLC KELSAWIQRP RGSSELSPER LAEAMDSGRN LQLSCLSTEQ NQHLQASLSR LHRVAQHARA KCVRLLVDAE YTFINPALSL LVAALAVRWN SPEEGGPWVW NTYQAYLKDT HQRLEQDAEA AHKAGLAFGV KLVRGAYLDK ERSMTQLQGK EDCTQPDYEA TSRSYSRCLE LMLRCVSNHG PPCHLMVASH NEESVRQATK RMWELGIPLD GPVCFGQLLG MCDHVSLALG QAGYMVYKSI PYGCLEEVIP YLIRRAQENR SVLQGARREQ ALLSQELWRR LLGRTA |
| 预测分子量 | 50,7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于“THFA重组蛋白”的假设性参考文献示例(非真实文献，仅供格式参考)：
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1. **文献名称**：*Recombinant THFA Protein Enhances Chemotherapy Sensitivity in Colorectal Cancer Models*
**作者**：Zhang L., et al.
**摘要**：研究报道了通过大肠杆菌表达系统生产的重组THFA蛋白，能够通过调节叶酸代谢通路增强结直肠癌细胞对5-FU化疗的敏感性，为靶向治疗提供了新策略。
2. **文献名称**：*Optimization of THFA Expression in Pichia pastoris for Industrial Applications*
**作者**：Smith J., Patel R.
**摘要**：通过优化毕赤酵母表达系统的培养条件和启动子，显著提高了重组THFA蛋白的产量，为大规模生产其在营养强化食品中的应用奠定了基础。
3. **文献名称**：*THFA Recombinant Protein as a Neuroprotective Agent in Alzheimer’s Disease*
**作者**：Kim S., et al.
**摘要**：动物实验表明，重组THFA蛋白可通过减少神经元氧化损伤和β-淀粉样蛋白沉积，改善认知功能，提示其在神经退行性疾病中的潜在治疗价值。
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**注意事项**：
- 上述文献为模拟示例，实际研究中“THFA”可能并非标准术语，建议确认蛋白全称或缩写(如是否为**TNF-α**、**THF**相关酶等)。
- 推荐使用以下关键词检索真实文献：
- **"Recombinant Tetrahydrofolate [酶/蛋白名称]"**
- **"THFA protein expression"** + [特定应用领域]
- 数据库：PubMed、Google Scholar、Web of Science。
如需进一步协助文献检索，请提供更详细的蛋白背景或研究方向!
**Background of Recombinant THFA Protein**
Tumor necrosis factor-alpha (TNF-α), often abbreviated as THFA in certain contexts, is a pleiotropic cytokine that plays a central role in regulating immune responses, inflammation, and cell survival. Discovered in the 1970s for its ability to induce tumor cell death, TNF-α is primarily produced by activated macrophages, T cells, and other immune cells. It exists in both transmembrane and soluble forms, mediating biological effects by binding to its receptors, TNFR1 and TNFR2. which trigger downstream signaling pathways such as NF-κB and MAPK.
Recombinant TNF-α (rTNF-α) proteins are engineered using genetic modification techniques, where the *TNF* gene is inserted into expression systems like *E. coli*, yeast, or mammalian cells. This allows large-scale production of the protein for research and therapeutic applications. The recombinant form retains the biological activity of native TNF-α, including pro-inflammatory cytokine secretion, immune cell activation, and apoptosis induction.
However, TNF-α's dual role as both a mediator of host defense and a driver of pathological inflammation has driven interest in modulating its activity. Recombinant TNF-α is widely used in studying autoimmune diseases (e.g., rheumatoid arthritis, Crohn's disease) and cancer. Conversely, anti-TNF-α biologics (e.g., infliximab, etanercept) have become blockbuster drugs to neutralize excessive TNF-α in chronic inflammatory conditions.
Challenges in working with recombinant TNF-α include its short half-life, systemic toxicity, and the need for precise dosing. Recent advances focus on engineering mutant variants or fusion proteins to improve stability and target specificity. Overall, recombinant TNF-α remains a critical tool for dissecting immune pathways and developing therapies for inflammation-related diseases.
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