| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | aMACR |
| Uniprot No | Q9UHK6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-382aa |
| 氨基酸序列 | ALQGISVVE LSGLAPGPFC AMVLADFGAR VVRVDRPGSR YDVSRLGRGK RSLVLDLKQP RGAAVLRRLC KRSDVLLEPF RRGVMEKLQL GPEILQRENP RLIYARLSGF GQSGSFCRLA GHDINYLALS GVLSKIGRSG ENPYAPLNLL ADFAGGGLMC ALGIIMALFD RTRTGKGQVI DANMVEGTAY LSSFLWKTQK LSLWEAPRGQ NMLDGGAPFY TTYRTADGEF MAVGAIEPQF YELLIKGLGL KSDELPNQMS MDDWPEMKKK FADVFAEKTK AEWCQIFDGT DACVTPVLTF EEVVHHDHNK ERGSFITSEE QDVSPRPAPL LLNTPAIPSF KRDPFIGEHT EEILEEFGFS REEIYQLNSD KIIESNKVKA SL |
| 预测分子量 | 42,3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于AMACR(Alpha-Methylacyl-CoA Racemase)重组蛋白的3篇代表性文献及其摘要概括：
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1. **文献名称**：*"Expression and Purification of Recombinant Human AMACR for Structural and Functional Studies"*
**作者**：Li, Y., et al.
**摘要**：本研究报道了人源AMACR重组蛋白在大肠杆菌中的高效表达及纯化方法，通过X射线晶体学解析其三维结构，揭示了其催化底物选择性机制，为前列腺癌相关药物设计提供结构基础。
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2. **文献名称**：*"Recombinant AMACR as a Diagnostic Marker in Prostate Cancer: Validation in Clinical Samples"*
**作者**：Kumar, S., & Thompson, R.H.
**摘要**：利用重组AMACR蛋白制备特异性抗体，验证其在组织切片中的表达水平与前列腺癌分期的相关性，证实AMACR可作为可靠的肿瘤标志物，提升病理诊断准确性。
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3. **文献名称**：*"Enzymatic Characterization of Recombinant AMACR Variants Associated with Peroxisomal Disorders"*
**作者**：Ferdinandusse, S., et al.
**摘要**：通过重组表达多种AMACR突变体，分析其酶活缺陷与过氧化物酶体代谢疾病的关系，发现特定氨基酸突变导致底物结合能力下降，为遗传性疾病的分子机制提供新见解。
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**备注**：以上文献为示例性质，实际研究中建议通过PubMed或Web of Science以“AMACR recombinant”、“Alpha-Methylacyl-CoA Racemase expression”为关键词检索最新成果。
**Background of aMACR Recombinant Protein**
The aMACR (artificially Modified Acyl-CoA Reductase) recombinant protein is an engineered enzyme designed to enhance catalytic efficiency and stability in industrial and research applications. Derived from native acyl-CoA reductases, which play crucial roles in lipid metabolism by reducing acyl-CoA to fatty alcohols, aMACR is optimized through directed evolution or rational protein design. These modifications often target substrate binding affinity, cofactor specificity (e.g., NADPH/NADH), and thermostability, making it adaptable to diverse bioprocessing conditions.
Recombinant production involves cloning the modified *amacr* gene into expression vectors (e.g., *E. coli* or yeast systems), followed by fermentation and purification via affinity chromatography. This ensures high yield and purity, critical for scalable applications. aMACR’s versatility is leveraged in biofuel production, where it converts fatty acids into alcohols for biodiesel synthesis, and in biotechnology for generating lipid-derived biomaterials. Its engineered traits also support pharmaceutical research, particularly in synthesizing wax esters or lipid-based drug carriers.
Unlike wild-type reductases, aMACR exhibits reduced substrate inhibition and improved activity under non-physiological conditions (e.g., high pH or organic solvents), addressing bottlenecks in industrial enzymology. Ongoing research focuses on further tailoring its properties via computational modeling and machine learning, aiming to expand its utility in sustainable chemistry and synthetic biology. As a customizable biocatalyst, aMACR exemplifies the convergence of protein engineering and industrial biotechnology to address energy and material challenges.
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