| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SEMG2 |
| Uniprot No | Q02383 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-582aa |
| 氨基酸序列 | MKSIILFVLSLLLILEKQAAVMGQKGGSKGQLPSGSSQFPHGQKGQHYFGQKDQQHTKSKGSFSIQHTYHVDINDHDWTRKSQQYDLNALHKATKSKQHLGGSQQLLNYKQEGRDHDKSKGHFHMIVIHHKGGQAHHGTQNPSQDQGNSPSGKGLSSQCSNTEKRLWVHGLSKEQASASGAQKGRTQGGSQSSYVLQTEELVVNKQQRETKNSHQNKGHYQNVVDVREEHSSKLQTSLHPAHQDRLQHGPKDIFTTQDELLVYNKNQHQTKNLSQDQEHGRKAHKISYPSSRTEERQLHHGEKSVQKDVSKGSISIQTEEKIHGKSQNQVTIHSQDQEHGHKENKISYQSSSTEERHLNCGEKGIQKGVSKGSISIQTEEQIHGKSQNQVRIPSQAQEYGHKENKISYQSSSTEERRLNSGEKDVQKGVSKGSISIQTEEKIHGKSQNQVTIPSQDQEHGHKENKMSYQSSSTEERRLNYGGKSTQKDVSQSSISFQIEKLVEGKSQIQTPNPNQDQWSGQNAKGKSGQSADSKQDLLSHEQKGRYKQESSESHNIVITEHEVAQDDHLTQQYNEDRNPIST |
| 预测分子量 | 66.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SEMG2重组蛋白的3篇参考文献示例(注：文献信息为模拟概括，实际引用需核实原文)：
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1. **文献名称**: "Expression and functional characterization of recombinant human Semenogelin II in a mammalian cell system"
**作者**: Tanaka K. et al.
**摘要**: 研究报道了在HEK293细胞中重组表达人SEMG2蛋白的方法，分析了其与精液凝固相关的自组装特性及对精子活力的抑制作用，为男性生殖研究提供工具蛋白。
2. **文献名称**: "Purification and structural analysis of SEMG2: implications for its role in seminal plasma"
**作者**: Chen L., Wang Y., Smith J.
**摘要**: 通过大肠杆菌表达系统获得高纯度重组SEMG2.利用质谱和圆二色谱解析其结构，发现其C端结构域在形成纤维网络中起关键作用，可能与不育症相关。
3. **文献名称**: "SEMG2 interacts with prostate-specific antigen (PSA) to regulate liquefaction of human semen"
**作者**: de Souza F.P. et al.
**摘要**: 研究通过重组SEMG2与PSA的体外互作实验，揭示了SEMG2作为PSA底物的降解机制，阐明其在精液液化过程中的动态调控功能。
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建议通过PubMed或Web of Science以关键词“recombinant SEMG2”、“semenogelin II expression”检索最新文献获取准确信息。
SEMG2 (Semenogelin II) is a key protein component of human seminal plasma, primarily involved in the formation and liquefaction of the seminal coagulum. It is synthesized in the seminal vesicles and secreted into semen alongside its homologous protein SEMG1. Both SEMG1 and SEMG2 interact with transglutaminase to form a fibrous network during ejaculation, temporarily immobilizing spermatozoa to protect them from the acidic vaginal environment. Over time, prostate-specific antigen (PSA) proteolytically cleaves these proteins, enabling sperm activation and motility. SEMG2 is characterized by its high molecular weight, cysteine-rich structure, and propensity for polymerization, though it exhibits a more complex glycosylation pattern compared to SEMG1.
Recombinant SEMG2 is produced using biotechnological platforms (e.g., bacterial, yeast, or mammalian expression systems) to enable controlled study of its biochemical properties and biological functions. The recombinant form bypasses ethical and purity challenges associated with isolating SEMG2 directly from seminal fluid. Researchers employ techniques like affinity chromatography and tag-based purification to obtain high-purity SEMG2 for experimental applications. Its recombinant version has become crucial for investigating semen biology, particularly in understanding male infertility linked to abnormal seminal coagulation/liquefaction. Additionally, SEMG2 is being explored as a potential biomarker for prostate-related pathologies and as a therapeutic target. Recent studies also suggest roles beyond reproduction, including antimicrobial activity and modulation of cervical immune responses. The development of recombinant SEMG2 continues to advance research in reproductive health, proteolytic regulation, and glycoprotein biochemistry.
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