| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FFAR4 |
| Uniprot No | Q5NUL3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-361aa |
| 氨基酸序列 | MSPECARAAGDAPLRSLEQANRTRFPFFSDVKGDHRLVLAAVETTVLVLIFAVSLLGNVCALVLVARRRRRGATACLVLNLFCADLLFISAIPLVLAVRWTEAWLLGPVACHLLFYVMTLSGSVTILTLAAVSLERMVCIVHLQRGVRGPGRRARAVLLALIWGYSAVAALPLCVFFRVVPQRLPGADQEISICTLIWPTIPGEISWDVSFVTLNFLVPGLVIVISYSKILQITKASRKRLTVSLAYSESHQIRVSQQDFRLFRTLFLLMVSFFIMWSPIIITILLILIQNFKQDLVIWPSLFFWVVAFTFANSALNPILYNMTLCRNEWKKIFCCFWFPEKGAILTDTSVKRNDLSIISG |
| 预测分子量 | 40,4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于FFAR4(GPR120)重组蛋白的3篇文献示例(虚构内容，仅供参考)：
1. **《FFAR4重组受体在炎症调控中的功能研究》**
*作者：Oh DY, et al.*
摘要：本研究通过大肠杆菌系统表达了人源FFAR4重组蛋白，证实其与ω-3脂肪酸结合后可抑制巨噬细胞中NF-κB通路，揭示了FFAR4在抗炎信号中的结构-功能关系。
2. **《GPR120重组蛋白的晶体结构解析与配体结合机制》**
*作者：Watson SJ, et al.*
摘要：利用昆虫细胞表达系统获得高纯度FFAR4重组蛋白，通过X射线衍射解析其三维结构，发现特定跨膜结构域是长链脂肪酸结合的关键位点。
3. **《基于FFAR4重组受体的高通量药物筛选平台构建》**
*作者：Milligan G, et al.*
摘要：开发了基于HEK293细胞稳定表达FFAR4重组蛋白的筛选模型，成功鉴定出新型选择性激动剂，为代谢性疾病药物研发提供工具。
(注：以上文献为模拟内容，实际研究中建议通过PubMed或Web of Science以"FFAR4 recombinant protein"或"GPR120 expression"为关键词检索最新论文。)
FFAR4 (Free Fatty Acid Receptor 4), also known as GPR120. is a G protein-coupled receptor (GPCR) that plays a critical role in sensing dietary fatty acids and regulating metabolic and inflammatory responses. It is activated by medium- to long-chain unsaturated fatty acids, such as omega-3 fatty acids, which are linked to anti-inflammatory and insulin-sensitizing effects. FFAR4 is expressed in various tissues, including adipose tissue, the gastrointestinal tract, and immune cells, where it modulates processes like hormone secretion, appetite regulation, and immune homeostasis. Its involvement in metabolic pathways has made it a promising therapeutic target for obesity, diabetes, and inflammatory diseases.
Recombinant FFAR4 protein is produced using genetic engineering techniques, typically expressed in mammalian or insect cell systems to ensure proper post-translational modifications and functional folding. This engineered protein retains ligand-binding capabilities and downstream signaling activity, enabling researchers to study its structure, activation mechanisms, and interactions with potential drugs. Purified FFAR4 is often tagged with markers like His or FLAG for isolation and detection. Applications include high-throughput screening of agonists/antagonists, structural studies (e.g., crystallography), and elucidating signaling cascades involving β-arrestin or Gαq pathways. The development of recombinant FFAR4 has accelerated research into its dual role in nutrient sensing and inflammation, offering insights for designing novel therapies targeting metabolic disorders.
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