| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | AP2m1 |
| Uniprot No | Q96CW1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-435aa |
| 氨基酸序列 | MIGGLFIYNHKGEVLISRVYRDDIGRNAVDAFRVNVIHARQQVRSPVTNIARTSFFHVKRSNIWLAAVTKQNVNAAMVFEFLYKMCDVMAAYFGKISEENIKNNFVLIYELLDEILDFGYPQNSETGALKTFITQQGIKSQHQTKEEQSQITSQVTGQIGWRREGIKYRRNELFLDVLESVNLLMSPQGQVLSAHVSGRVVMKSYLSGMPECKFGMNDKIVIEKQGKGTADETSKSGKQSIAIDDCTFHQCVRLSKFDSERSISFIPPDGEFELMRYRTTKDIILPFRVIPLVREVGRTKLEVKVVIKSNFKPSLLAQKIEVRIPTPLNTSGVQVICMKGKAKYKASENAIVWKIKRMAGMKESQISAEIELLPTNDKKKWARPPISMNFEVPFAPSGLKVRYLKVFEPKLNYSDHDVIKWVRYIGRSGIYETRC |
| 预测分子量 | 53.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于AP2m1重组蛋白的3篇参考文献示例(内容为模拟生成，非真实文献)：
1. **文献名称**：*Structural insights into AP2m1 function in clathrin-mediated endocytosis*
**作者**：Smith A.B., Jones C.D.
**摘要**：本研究通过重组表达AP2m1蛋白，利用X射线晶体学解析其三维结构，揭示了其与clathrin及受体尾部相互作用的分子机制，为内吞途径的调控提供结构基础。
2. **文献名称**：*AP2m1重组蛋白在体外结合实验中的功能表征*
**作者**：Wang X., Zhang L.
**摘要**：通过原核系统表达并纯化AP2m1重组蛋白，验证其与β2-adaptin及细胞膜受体的结合能力，证实AP2m1在适配体复合物组装中的关键作用。
3. **文献名称**：*AP2m1 knock-down and rescue experiments using recombinant protein in neuronal cells*
**作者**：Brown E.F., Lee S.
**摘要**：利用重组AP2m1蛋白恢复神经元细胞的内吞功能，证明其在突触囊泡循环中的必要性，并发现特定磷酸化位点影响其与配体的结合效率。
(注：以上文献为示例，实际引用请通过PubMed、Google Scholar等平台搜索真实文献。)
**Background of AP2m1 Recombinant Protein**
AP2m1 (Adaptor Protein 2 mu subunit 1) is a critical component of the heterotetrameric AP-2 complex, a central player in clathrin-mediated endocytosis (CME). This evolutionarily conserved process enables cells to internalize extracellular molecules, membrane receptors, and lipids by forming clathrin-coated vesicles. The AP-2 complex acts as a scaffold, coordinating cargo recognition, membrane bending, and clathrin assembly. Among its four subunits (α, β2. μ, σ), AP2m1 (μ subunit) directly binds to tyrosine-based sorting motifs (e.g., YXXΦ) on cargo proteins, such as transmembrane receptors (e.g., transferrin receptor, EGFR), ensuring their selective recruitment into vesicles.
Recombinant AP2m1 protein is engineered to study the molecular mechanisms of endocytosis and cargo sorting. Produced via expression systems like *E. coli* or mammalian cells, it retains the ability to interact with cargo motifs and other endocytic components. Researchers use it in *in vitro* assays to dissect AP-2’s role in vesicle formation, screen for endocytosis inhibitors, or analyze mutations linked to diseases. For instance, AP2m1 dysfunction is associated with neurodevelopmental disorders, cancer metastasis, and viral entry mechanisms (e.g., HIV, SARS-CoV-2), as pathogens often hijack CME for infection.
Structural studies employing recombinant AP2m1 have revealed conformational changes during cargo binding and regulatory phosphorylation sites, offering insights into therapeutic targeting. Its application extends to drug discovery, particularly in diseases with disrupted endocytic trafficking, emphasizing its biomedical relevance.
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