| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SCNN1b |
| Uniprot No | P51168 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-640aa |
| 氨基酸序列 | MHVKKYLLKGLHRLQKGPGYTYKELLVWYCDNTNTHGPKRIICEGPKKKAMWFLLTLLFAALVCWQWGIFIRTYLSWEVSVSLSVGFKTMDFPAVTICNASPFKYSKIKHLLKDLDELMEAVLERILAPELSHANATRNLNFSIWNHTPLVLIDERNPHHPMVLDLFGDNHNGLTSSSASEKICNAHGCKMAMRLCSLNRTQCTFRNFTSATQALTEWYILQATNIFAQVPQQELVEMSYPGEQMILACLFGAEPCNYRNFTSIFYPHYGNCYIFNWGMTEKALPSANPGTEFGLKLILDIGQEDYVPFLASTAGVRLMLHEQRSYPFIRDEGIYAMSGTETSIGVLVDKLQRMGEPYSPCTVNGSEVPVQNFYSDYNTTYSIQACLRSCFQDHMIRNCNCGHYLYPLPRGEKYCNNRDFPDWAHCYSDLQMSVAQRETCIGMCKESCNDTQYKMTISMADWPSEASEDWIFHVLSQERDQSTNITLSRKGIVKLNIYFQEFNYRTIEESAANNIVWLLSNLGGQFGFWMGGSVLCLIEFGEIIIDFVWITIIKLVALAKSLRQRRAQASYAGPPPTVAELVEAHTNFGFQPDTAPRSPNTGPYPSEQALPIPGTPPPNYDSLRLQPLDVIESDSEGDAI |
| 预测分子量 | 72,6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SCNN1B重组蛋白的3篇参考文献示例(注：文献为模拟示例，非真实存在)：
1. **文献名称**：重组人SCNN1B蛋白在HEK293细胞中的表达与功能分析
**作者**：Smith A, et al.
**摘要**：该研究通过构建SCNN1B重组表达载体，在HEK293细胞中成功表达并纯化出功能性SCNN1B蛋白。实验表明，重组蛋白可与其他ENaC亚基组装形成离子通道，并证实其在钠离子转运中的关键作用。
2. **文献名称**：SCNN1B重组蛋白的晶体结构解析及其对药物结合的影响
**作者**：Zhang L, et al.
**摘要**：研究者利用X射线晶体学解析了SCNN1B重组蛋白的高分辨率结构，揭示了其与阿米洛利(amiloride)类药物的结合位点，为开发针对ENaC通道的抑制剂提供了结构基础。
3. **文献名称**：SCNN1B基因突变对重组蛋白稳定性的影响及疾病关联研究
**作者**：Johnson R, et al.
**摘要**：通过体外突变体重组蛋白表达，研究发现某些遗传性高血压相关的SCNN1B突变会导致蛋白稳定性下降，进而影响ENaC通道的活性，揭示了其与疾病的分子机制关联。
(注：以上文献为示例，实际文献需通过PubMed、Google Scholar等平台检索获取。)
**Background of SCNN1B Recombinant Protein**
The SCNN1B gene encodes the beta (β) subunit of the epithelial sodium channel (ENaC), a membrane-bound ion channel critical for sodium reabsorption and fluid homeostasis. ENaC is composed of three homologous subunits—α, β, and γ (encoded by SCNN1A, SCNN1B, and SCNN1G, respectively)—that assemble to form a functional channel. The β-subunit, produced by the SCNN1B gene, plays a key regulatory role in channel activity, trafficking, and stability. ENaC is predominantly expressed in epithelial tissues, such as the kidneys, lungs, and colon, where it regulates sodium transport, impacting blood pressure, airway surface liquid homeostasis, and electrolyte balance.
SCNN1B recombinant protein is engineered through molecular cloning and expression systems (e.g., mammalian or bacterial cells) to produce purified β-subunits for *in vitro* studies. This recombinant tool enables researchers to investigate ENaC’s structure-function relationships, modulation by hormones (e.g., aldosterone), and interactions with regulatory proteins (e.g., proteases, ubiquitin ligases). Dysregulation of ENaC, often linked to SCNN1B mutations, is associated with diseases such as Liddle syndrome (hypertension due to hyperactive ENaC) and pseudohypoaldosteronism type 1 (salt-wasting disorders).
The recombinant protein also aids in drug discovery, particularly for conditions like cystic fibrosis (targeting ENaC overactivity) or hypertension. By studying SCNN1B-derived subunits, researchers aim to develop therapies that modulate ENaC activity with high specificity, balancing therapeutic efficacy and minimizing off-target effects. Overall, SCNN1B recombinant protein serves as a vital resource for unraveling ENaC biology and its pathophysiological implications.
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