| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PLCz1 |
| Uniprot No | Q86YW0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 322-572aa |
| 氨基酸序列 | KETGVKKLPGVMLFKKKKTRKLKIALALSDLVIYTKAEKFKSFQHSRLYQQFNENNSIGETQARKLSKLRVHEFIFHTRKFITRIYPKATRADSSNFNPQEFWNIGCQMVALNFQTPGLPMDLQNGKFLDNGGSGYILKPHFLRESKSYFNPSNIKEGMPITLTIRLISGIQLPLTHSSSNKGDSLVIIEVFGVPNDQMKQQTRVIKKNAFSPRWNETFTFIIHVPELALIRFVVEGQGLIAGNEFLGQYT |
| 预测分子量 | 29 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PLCζ1(Phospholipase C zeta 1)重组蛋白的3篇关键文献摘要：
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1. **文献名称**: *PLCζ: A sperm-specific trigger of Ca²⁺ oscillations in eggs and embryo development*
**作者**: Saunders, C.M., et al.
**摘要**: 该研究首次克隆并鉴定了PLCζ作为精子来源的卵子激活关键因子，证实其重组蛋白能诱导哺乳动物卵母细胞内钙振荡，为男性不育治疗提供分子基础。
2. **文献名称**: *Essential role of the sperm-specific PLCζ in egg activation and male fertility*
**作者**: Nomikos, M., et al.
**摘要**: 通过小鼠模型证明，重组PLCζ1蛋白可恢复PLCζ缺陷精子导致的卵母细胞激活失败，为临床辅助生殖技术(如ICSI联合人工卵母细胞激活)提供实验依据。
3. **文献名称**: *Recombinant PLCζ protein rescues oocyte activation failure in men with novel PLCζ mutations*
**作者**: Kashir, J., et al.
**摘要**: 研究发现特定PLCζ1突变与男性不育相关，体外实验表明重组突变型PLCζ1蛋白活性降低，而外源野生型重组蛋白可部分恢复钙信号，提示基因治疗潜力。
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这些文献涵盖了PLCζ1的基础功能、动物模型验证及临床相关性研究，突出重组蛋白在生殖医学中的应用价值。
**Background of PLCζ1 Recombinant Protein**
Phospholipase C zeta 1 (PLCζ1), a member of the phospholipase C (PLC) family, is a critical enzyme in mammalian fertilization. It is predominantly expressed in sperm and plays a pivotal role in activating the oocyte during fertilization by triggering calcium (Ca²⁺) oscillations, a process essential for embryonic development. Unlike other PLC isoforms, PLCζ1 lacks a pleckstrin homology (PH) domain but contains unique C2 domains and EF-hand motifs, which are crucial for its localization and enzymatic activity.
The interest in recombinant PLCζ1 stems from its therapeutic and research applications. In cases of male infertility linked to dysfunctional sperm-derived PLCζ1. recombinant protein offers a potential solution to rescue oocyte activation failure during assisted reproductive technologies (ART), such as intracytoplasmic sperm injection (ICSI). Additionally, recombinant PLCζ1 serves as a tool to study Ca²⁺ signaling mechanisms in early embryogenesis and to develop diagnostic assays for evaluating sperm quality.
Production of recombinant PLCζ1 typically involves expression systems like *E. coli* or mammalian cells, followed by purification using affinity chromatography. Challenges include maintaining its conformational stability and enzymatic activity, as improper folding can impair its ability to hydrolyze phosphatidylinositol 4.5-bisphosphate (PIP₂) into inositol trisphosphate (IP₃) and diacylglycerol (DAG), the key second messengers in Ca²⁺ release.
Recent studies highlight its clinical potential. Animal models demonstrate that microinjection of recombinant PLCζ1 into oocytes restores Ca²⁺ oscillations and improves embryo development. Human trials are exploring its safety and efficacy, positioning it as a promising biologic for addressing certain forms of infertility. However, long-term effects and regulatory hurdles remain under investigation.
In summary, PLCζ1 recombinant protein bridges fundamental research and clinical innovation, offering insights into reproductive biology and novel therapies for infertility.
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