| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FNTa |
| Uniprot No | P49354 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-379aa |
| 氨基酸序列 | AATEGVGEA AQGGEPGQPA QPPPQPHPPP PQQQHKEEMA AEAGEAVASP MDDGFVSLDS PSYVLYRDRA EWADIDPVPQ NDGPNPVVQI IYSDKFRDVY DYFRAVLQRD ERSERAFKLT RDAIELNAAN YTVWHFRRVL LKSLQKDLHE EMNYITAIIE EQPKNYQVWH HRRVLVEWLR DPSQELEFIA DILNQDAKNY HAWQHRQWVI QEFKLWDNEL QYVDQLLKED VRNNSVWNQR YFVISNTTGY NDRAVLEREV QYTLEMIKLV PHNESAWNYL KGILQDRGLS KYPNLLNQLL DLQPSHSSPY LIAFLVDIYE DMLENQCDNK EDILNKALEL CEILAKEKDT IRKEYWRYIG RSLQSKHSTE NDSPTNVQQ |
| 预测分子量 | 44,4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于FNTA(法尼基转移酶α亚基)重组蛋白的参考文献示例，这些示例基于常见研究方向构造，仅供参考。建议通过学术数据库(如PubMed、Google Scholar)检索最新真实文献：
---
1. **标题**：*"High-yield Expression and Functional Characterization of Recombinant FNTA Protein in a Bacterial System"*
**作者**：Chen X, Li R, et al.
**摘要**：本研究建立了一种高效的大肠杆菌表达系统，用于生产重组FNTA蛋白。通过优化表达条件及亲和层析纯化步骤，获得了高纯度且具有酶活性的FNTA，为后续结构及功能研究奠定基础。
2. **标题**：*"Crystal Structure of FNTA Reveals Mechanistic Insights into Farnesyltransferase Activity"*
**作者**：Martinez D, Thompson A.
**摘要**：通过X射线晶体学解析了FNTA的三维结构，揭示了其与FNTB亚基相互作用的分子机制，并探讨了底物结合位点的关键氨基酸残基，为设计靶向抑制剂提供结构基础。
3. **标题**：*"Targeting FNTA in Ras-driven Cancers: Biochemical and Cellular Studies Using Recombinant Protein"*
**作者**：Kim H, Patel S.
**摘要**：利用重组FNTA蛋白进行体外酶活实验，筛选出新型小分子抑制剂，证明其可有效阻断Ras蛋白法尼基化，抑制肿瘤细胞生长，提示FNTA作为抗癌靶点的潜力。
4. **标题**：*"Role of FNTA in Neurodegenerative Diseases: Insights from Recombinant Protein-based Assays"*
**作者**：Gupta M, et al.
**摘要**：研究重组FNTA在神经细胞中的异常活性与tau蛋白异常修饰的关系，发现FNTA过表达可能加剧神经退行性病变，为相关疾病机制提供新视角。
---
**注意**：以上文献为示例性质，具体研究请以实际检索结果为准。推荐使用关键词 **"FNTA recombinant protein"** 或 **"Farnesyltransferase alpha subunit"** 在学术平台搜索最新成果。
FNTa (Formin-like protein 1. FMNL1) recombinant protein is a engineered protein derived from the formin family, which plays critical roles in cytoskeletal dynamics and cellular processes. Formins are actin-nucleating proteins that regulate the assembly of linear actin filaments, impacting cell motility, cytokinesis, and intracellular trafficking. FMNL1. a member of the diaphanous-related formin subfamily, is particularly associated with membrane protrusion, vesicle trafficking, and immune cell functions. Its structure includes formin homology domains (FH1 and FH2) essential for actin polymerization and interactions with regulatory proteins like Rho GTPases.
Recombinant FNTa production typically involves cloning the FMNL1 gene into expression systems (e.g., E. coli, mammalian cells) to generate purified, functional protein for research. This tool enables studies on actin-driven processes in cancer metastasis, immune cell migration, and neurological development. Dysregulation of FMNL1 is linked to diseases such as leukemia, glioblastoma, and chronic inflammation, making it a potential therapeutic target. Researchers use FNTa recombinant proteins to dissect molecular mechanisms, screen inhibitors, or explore its interplay with signaling pathways. Its applications extend to structural biology, drug discovery, and functional assays elucidating cytoskeletal remodeling in health and disease.
×
