| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SPTLC2 |
| Uniprot No | O15270 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 88-562aa |
| 氨基酸序列 | RDFLRYWRIEKCHHATEREEQKDFVSLYQDFENFYTRNLYMRIRDNWNRPICSVPGARVDIMERQSHDYNWSFKYTGNIIKGVINMGSYNYLGFARNTGSCQEAAAKVLEEYGAGVCSTRQEIGNLDKHEELEELVARFLGVEAAMAYGMGFATNSMNIPALVGKGCLILSDELNHASLVLGARLSGATIRIFKHNNMQSLEKLLKDAIVYGQPRTRRPWKKILILVEGIYSMEGSIVRLPEVIALKKKYKAYLYLDEAHSIGALGPTGRGVVEYFGLDPEDVDVMMGTFTKSFGASGGYIGGKKELIDYLRTHSHSAVYATSLSPPVVEQIITSMKCIMGQDGTSLGKECVQQLAENTRYFRRRLKEMGFIIYGNEDSPVVPLMLYMPAKIGAFGREMLKRNIGVVVVGFPATPIIESRARFCLSAAHTKEILDTALKEIDEVGDLLQLKYSRHRLVPLLDRPFDETTYEETED |
| 预测分子量 | 69.7kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SPTLC2重组蛋白的3篇代表性文献概览：
1. **文献名称**：*"Expression and characterization of recombinant human serine palmitoyltransferase complexes: Sptlc2 is the key regulatory subunit"*
**作者**：Yasuko et al.
**摘要**：通过在大肠杆菌和哺乳动物细胞中重组表达SPTLC1/SPTLC2复合体，发现SPTLC2是调控丝氨酸棕榈酰转移酶(SPT)活性的关键亚基，其突变会显著降低鞘脂合成的催化效率。
2. **文献名称**：*"Structural basis of substrate recognition by SPTLC2-SPTSSA complex in sphingolipid biosynthesis"*
**作者**：Wang et al.
**摘要**：利用重组SPTLC2与伴侣蛋白SPTSSA的共表达，解析了复合体冷冻电镜结构，揭示了SPTLC2底物结合口袋的构象变化及在遗传性神经病变相关突变中的功能影响。
3. **文献名称**：*"Functional reconstitution of mammalian serine palmitoyltransferase with recombinant SPTLC2 variants linked to hereditary sensory neuropathy"*
**作者**：Gable et al.
**摘要**：通过重组SPTLC2突变体与SPTLC1的体外功能重建，证明特定突变(如C133W)导致SPT酶异常底物选择性，进而引发神经鞘脂代谢紊乱和神经元功能障碍。
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**说明**：以上文献聚焦于SPTLC2重组蛋白的表达系统开发、结构功能解析及其在疾病中的机制。研究多采用复合体重组表达策略，结合生化和结构生物学手段，阐明其在鞘脂代谢中的核心作用。
**Background of SPTLC2 Recombinant Protein**
SPTLC2 (serine palmitoyltransferase long-chain subunit 2) is a critical enzyme subunit within the serine palmitoyltransferase (SPT) complex, which catalyzes the first and rate-limiting step in sphingolipid biosynthesis. Sphingolipids are essential components of cellular membranes and play vital roles in signaling, apoptosis, and membrane integrity. The SPT complex typically consists of two main subunits, SPTLC1 and SPTLC2 (or its paralog SPTLC3), which together condense serine and palmitoyl-CoA to form 3-ketodihydrosphingosine, a precursor for all sphingolipids. SPTLC2 specifically contributes the catalytic site for substrate binding and determines the acyl-CoA chain length specificity, influencing sphingolipid diversity.
Dysregulation of SPTLC2 is linked to metabolic disorders, neurodegenerative diseases, and rare genetic conditions like hereditary sensory and autonomic neuropathy type 1 (HSAN1), where mutations lead to toxic sphingolipid accumulation. Studying SPTLC2’s structure and function is crucial for understanding these pathologies and developing targeted therapies.
Recombinant SPTLC2 protein is produced using heterologous expression systems (e.g., *E. coli*, insect, or mammalian cells*) to generate purified, functional protein for research. Its production enables *in vitro* studies on enzyme kinetics, inhibitor screening, and structural analyses (e.g., X-ray crystallography or cryo-EM) to elucidate mechanisms of sphingolipid regulation. Tagged versions (e.g., His-tag) facilitate purification and interaction studies.
Researchers also use SPTLC2 recombinant protein to model disease-associated mutations, test corrective compounds, and explore its role in cellular processes like autophagy and stress responses. Its application extends to drug discovery, particularly for metabolic and neurological disorders, highlighting its biomedical significance.
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