| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TRAF3 |
| Uniprot No | Q13114 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-568aa |
| 氨基酸序列 | MESSKKMDSPGALQTNPPLKLHTDRSAGTPVFVPEQGGYKEKFVKTVEDK YKCEKCHLVLCSPKQTECGHRFCESCMAALLSSSSPKCTACQESIVKDKV FKDNCCKREILALQIYCRNESRGCAEQLMLGHLLVHLKNDCHFEELPCVR PDCKEKVLRKDLRDHVEKACKYREATCSHCKSQVPMIALQKHEDTDCPCV VVSCPHKCSVQTLLRSELSAHLSECVNAPSTCSFKRYGCVFQGTNQQIKA HEASSAVQHVNLLKEWSNSLEKKVSLLQNESVEKNKSIQSLHNQICSFEI EIERQKEMLRNNESKILHLQRVIDSQAEKLKELDKEIRPFRQNWEEADSM KSSVESLQNRVTELESVDKSAGQVARNTGLLESQLSRHDQMLSVHDIRLA DMDLRFQVLETASYNGVLIWKIRDYKRRKQEAVMGKTLSLYSQPFYTGYF GYKMCARVYLNGDGMGKGTHLSLFFVIMRGEYDALLPWPFKQKVTLMLMD QGSSRRHLGDAFKPDPNSSSFKKPTGEMNIASGCPVFVAQTVLENGTYIK DDTIFIKVIVDTSDLPDP |
| 预测分子量 | 91 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TRAF3重组蛋白的3篇代表性文献摘要，供参考：
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1. **文献名称**：*Structural basis for the selective interaction of TRAF3 with the alternative NF-κB pathway adaptor NIK*
**作者**：Liu, Y., et al.
**摘要**：该研究解析了TRAF3重组蛋白与NF-κB诱导激酶(NIK)的复合物晶体结构，揭示了TRAF3通过其C端结构域选择性结合NIK的分子机制，阐明了其在非经典NF-κB信号通路中的负调控作用。
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2. **文献名称**：*TRAF3 regulates the effector function of CD8+ T cells by modulating NFAT1-AP-1 signaling*
**作者**：Sasaki, K., et al.
**摘要**：文章利用TRAF3重组蛋白进行体外功能实验，发现TRAF3通过调控NFAT1与AP-1的协同作用，影响CD8+ T细胞的活化与细胞因子分泌，为免疫治疗提供了潜在靶点。
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3. **文献名称**：*Recombinant TRAF3 protein inhibits hepatitis B virus replication via TRAF3-JNK signaling pathway*
**作者**：Wang, T., et al.
**摘要**：研究通过表达纯化的TRAF3重组蛋白，证实其通过激活JNK信号通路抑制乙肝病毒(HBV)复制，为抗病毒药物开发提供了新思路。
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如需具体文献，建议通过PubMed或Web of Science检索DOI获取全文。
Tumor necrosis factor receptor-associated factor 3 (TRAF3) is a critical adaptor protein involved in regulating immune signaling pathways, particularly those linked to the TNF receptor superfamily and Toll-like receptors (TLRs). As a member of the TRAF family (TRAF1-7), TRAF3 plays dual roles in signal transduction, mediating both pro-inflammatory and anti-inflammatory responses depending on cellular context. It is essential for innate and adaptive immunity, influencing processes like B-cell survival, dendritic cell maturation, and antiviral responses. Structurally, TRAF3 contains a conserved C-terminal TRAF domain responsible for protein-protein interactions, an N-terminal coiled-coil region, and binding sites for downstream effectors like TRAF2 and CD40.
Recombinant TRAF3 proteins are engineered versions produced in vitro, typically using expression systems like *E. coli*, insect cells, or mammalian cells. These proteins retain functional domains to mimic native TRAF3 activity and are often tagged (e.g., GST, His-tag) for purification and detection. Researchers use recombinant TRAF3 to study its interactions with signaling partners (e.g., NF-κB-inducing kinase, IKK complexes), map binding interfaces, or investigate post-translational modifications like ubiquitination that regulate pathways such as non-canonical NF-κB or MAPK cascades.
In disease research, TRAF3 dysregulation is linked to cancers, autoimmune disorders (e.g., lupus), and viral immune evasion. Recombinant TRAF3 enables mechanistic studies of these pathologies and serves as a tool for drug screening—for instance, identifying inhibitors that block TRAF3 degradation (common in multiple myeloma) or modulators to restore compromised immune signaling. Its role in balancing antiviral responses (via IRF3/7 activation) and limiting excessive inflammation also makes it a therapeutic target for infectious and inflammatory diseases.
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