| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RPA1 |
| Uniprot No | P27694 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-616aa |
| 氨基酸序列 | VGQLSEGAIAAIMQKGDTNIKPILQVINIRPITTGNSPPRYRLLMSDGLNTLSSFMLATQLNPLVEEEQLSSNCVCQIHRFIVNTLKDGRRVVILMELEVLKSAEAVGVKIGNPVPYNEGLGQPQVAPPAPAASPAASSRPQPQNGSSGMGSTVSKAYGASKTFGKAAGPSLSHTSGGTQSKVVPIASLTPYQSKWTICARVTNKSQIRTWSNSRGEGKLFSLELVDESGEIRATAFNEQVDKFFPLIEVNKVYYFSKGTLKIANKQFTAVKNDYEMTFNNETSVMPCEDDHHLPTVQFDFTGIDDLENKSKDSLVDIIGICKSYEDATKITVRSNNREVAKRNIYLMDTSGKVVTATLWGEDADKFDGSRQPVLAIKGARVSDFGGRSLSVLSSSTIIANPDIPEAYKLRGWFDAEGQALDGVSISDLKSGGVGGSNTNWKTLYEVKSENLGQGDKPDYFSSVATVVYLRKENCMYQACPTQDCNKKVIDQQNGLYRCEKCDTEFPNFKYRMILSVNIADFQENQWVTCFQESAEAILGQNAAYLGELKDKNEQAFEEVFQNANFRSFIFRVRVKVETYNDESRIKATVMDVKPVDYREYGRRLVMSIRRSALM |
| 预测分子量 | 84.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RPA1重组蛋白的3篇参考文献示例(内容为模拟概括，建议根据实际文献调整)：
1. **文献名称**: "Recombinant Human Replication Protein A1: Overexpression in Escherichia coli and Functional Characterization"
**作者**: Smith J., et al.
**摘要**: 该研究报道了人源RPA1亚基在大肠杆菌中的高效重组表达及纯化方法，验证了重组蛋白在体外的DNA结合活性，并证明其与RPA2/RPA3亚基形成复合物后可恢复DNA损伤修复功能。
2. **文献名称**: "Structural Insights into RPA1’s Role in DNA Repair via Crystallography of Recombinant Protein Complexes"
**作者**: Chen L., et al.
**摘要**: 通过重组表达RPA1蛋白并进行晶体结构解析，揭示了其在DNA单链结合区域的关键构象变化，阐明了RPA1在非同源末端连接(NHEJ)修复途径中的分子机制。
3. **文献名称**: "Functional Analysis of RPA1 Phosphorylation Sites Using Recombinant Mutant Proteins"
**作者**: Wang Y., et al.
**摘要**: 研究利用重组技术构建了RPA1磷酸化位点突变体，发现特定位点的磷酸化修饰可调控RPA1与ATM/ATR激酶的相互作用，进而影响细胞周期检查点激活效率。
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注：以上为模拟内容，实际文献需通过PubMed/Google Scholar等平台以关键词“RPA1 recombinant protein”检索。如需具体文献，可提供发表年份或研究领域进一步筛选。
**Background of RPA1 Recombinant Protein**
Replication Protein A (RPA), a highly conserved single-stranded DNA-binding (SSB) protein complex, plays a central role in DNA metabolism, including replication, repair, recombination, and damage signaling. The RPA heterotrimer consists of three subunits: RPA1 (70 kDa), RPA2 (32 kDa), and RPA3 (14 kDa). Among these, RPA1 is the largest subunit and serves as the primary DNA-binding component. It contains multiple oligonucleotide/oligosaccharide-binding (OB) folds, which mediate interactions with ssDNA and other proteins critical for DNA processing.
RPA1’s structural domains, particularly the N-terminal DNA-binding domains (DBDs F, A, B, and C), enable high-affinity binding to ssDNA, stabilizing replication forks and preventing DNA degradation. Additionally, RPA1 recruits and coordinates key repair factors, such as RAD52 and XPA, during homologous recombination and nucleotide excision repair. Its role in the DNA damage response (DDR) involves activating checkpoint kinases like ATR, ensuring cell cycle arrest until lesions are resolved.
Recombinant RPA1 protein is engineered for *in vitro* studies to dissect its molecular functions. Produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), it retains biochemical activity and is purified using affinity chromatography. Quality assessments, including SDS-PAGE and functional assays (e.g., electrophoretic mobility shift assays), confirm its ssDNA-binding capacity and interaction with partner proteins.
Research applications include elucidating mechanisms of genome stability, cancer biology (e.g., RPA1 overexpression in tumors), and neurodegenerative diseases linked to RPA dysfunction. Recombinant RPA1 also aids in drug discovery, screening for inhibitors targeting DNA repair pathways to sensitize cancer cells to chemo- or radiotherapy. Understanding RPA1’s structure-function relationship remains vital for advancing therapies against diseases driven by genomic instability.
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