| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | b4GALNT2 |
| Uniprot No | Q8NHY0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-566aa |
| 氨基酸序列 | MGSAGFSVGKFHVEVASRGRECVSGTPECGNRLGSAGFGALCLELRGADPAWGPFAAHGRSRRQGSRFLWLLKILVIILVLGIVGFMFGSMFLQAVFSSPKPELPSPAPGVQKLKLLPEERLRNLFSYDGIWLFPKNQCKCEANKEQGGYNFQDAYGQSDLPAVKARRQAEFEHFQRREGLPRPLPLLVQPNLPFGYPVHGVEVMPLHTVPIPGLQFEGPDAPVYEVTLTASLGTLNTLADVPDSVVQGRGQKQLIISTSDRKLLKFILQHVTYTSTGYQHQKVDIVSLESRSSVAKFPVTIRHPVIPKLYDPGPERKLRNLVTIATKTFLRPHKLMIMLRSIREYYPDLTVIVADDSQKPLEIKDNHVEYYTMPFGKGWFAGRNLAISQVTTKYVLWVDDDFLFNEETKIEVLVDVLEKTELDVVGGSVLGNVFQFKLLLEQSENGACLHKRMGFFQPLDGFPSCVVTSGVVNFFLAHTERLQRVGFDPRLQRVAHSEFFIDGLGTLLVGSCPEVIIGHQSRSPVVDSELAALEKTYNTYRSNTLTRVQFKLALHYFKNHLQCAA |
| 预测分子量 | 63,2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于b4GALNT2重组蛋白的3篇文献摘要示例(注：文献为虚构概括，仅用于示例格式)：
1. **文献名称**：*Structural characterization of recombinant human b4GALNT2 and its role in ganglioside biosynthesis*
**作者**：Tanaka K, et al.
**摘要**：本研究成功在大肠杆菌中表达并纯化了重组人b4GALNT2蛋白，解析了其晶体结构，证实其通过催化GM2向GM1a的转化参与神经节苷脂代谢，为相关溶酶体贮积症研究提供分子基础。
2. **文献名称**：*b4GALNT2 overexpression promotes cancer cell adhesion and metastasis via integrin glycosylation*
**作者**：Chen L, et al.
**摘要**：通过重组b4GALNT2蛋白功能实验，发现其在结肠癌细胞中异常高表达，可修饰整合素β1的N-聚糖结构，增强肿瘤细胞黏附和转移能力，提示其作为癌症治疗潜在靶点。
3. **文献名称**：*Recombinant b4GALNT2 rescues neuromuscular junction defects in a zebrafish model*
**作者**：Smith J, et al.
**摘要**：利用斑马鱼模型证明重组b4GALNT2蛋白能恢复因基因突变导致的神经肌肉接头突触形成异常，揭示其在维持突触稳定性中的关键作用。
(注：如需真实文献，建议通过PubMed或Google Scholar检索关键词“b4GALNT2 recombinant protein”。)
**Background of b4GALNT2 Recombinant Protein**
The β-1.4-N-acetylgalactosaminyltransferase 2 (b4GALNT2) is a glycosyltransferase enzyme that catalyzes the transfer of N-acetylgalactosamine (GalNAc) to terminal galactose residues via a β-1.4 linkage, forming the Sd^a antigen—a carbohydrate structure prominently expressed on cell surfaces and secreted glycoproteins. This enzyme plays a critical role in glycosphingolipid biosynthesis and is implicated in modulating cell-cell interactions, pathogen recognition, and immune responses.
b4GALNT2 is predominantly expressed in the gastrointestinal tract, kidneys, and brain, with its activity linked to tissue-specific glycosylation patterns. Dysregulation of b4GALNT2 has been associated with diseases such as colorectal cancer, where altered glycosylation may influence tumor progression and metastasis, and paroxysmal nocturnal hemoglobinuria (PNH), due to its role in complement regulation. Additionally, genetic variants of *B4GALNT2* are linked to rare neurological disorders, underscoring its importance in neural development.
Recombinant b4GALNT2 protein, produced through heterologous expression systems like mammalian or insect cells, retains enzymatic activity and is widely used to study glycan biosynthesis mechanisms. It serves as a tool for *in vitro* glycoengineering to modify therapeutic proteins, enhancing their stability or targeting specificity. Researchers also employ it to investigate pathological glycosylation changes and develop biomarkers for cancer or immune-related conditions.
Overall, b4GALNT2 recombinant protein is pivotal in advancing glycobiology research, offering insights into disease mechanisms and enabling the design of glycotherapeutics with tailored glycosylation profiles.
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