| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RPS6Ka1 |
| Uniprot No | Q15418 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-735aa |
| 氨基酸序列 | MPLAQLKEPW PLMELVPLDP ENGQTSGEEA GLQPSKDEGV LKEISITHHV KAGSEKADPS HFELLKVLGQ GSFGKVFLVR KVTRPDSGHL YAMKVLKKAT LKVRDRVRTK MERDILADVN HPFVVKLHYA FQTEGKLYLI LDFLRGGDLF TRLSKEVMFT EEDVKFYLAE LALGLDHLHS LGIIYRDLKP ENILLDEEGH IKLTDFGLSK EAIDHEKKAY SFCGTVEYMA PEVVNRQGHS HSADWWSYGV LMFEMLTGSL PFQGKDRKET MTLILKAKLG MPQFLSTEAQ SLLRALFKRN PANRLGSGPD GAEEIKRHVF YSTIDWNKLY RREIKPPFKP AVAQPDDTFY FDTEFTSRTP KDSPGIPPSA GAHQLFRGFS FVATGLMEDD GKPRAPQAPL HSVVQQLHGK NLVFSDGYVV KETIGVGSYS ECKRCVHKAT NMEYAVKVID KSKRDPSEEI EILLRYGQHP NIITLKDVYD DGKHVYLVTE LMRGGELLDK ILRQKFFSER EASFVLHTIG KTVEYLHSQG VVHRDLKPSN ILYVDESGNP ECLRICDFGF AKQLRAENGL LMTPCYTANF VAPEVLKRQG YDEGCDIWSL GILLYTMLAG YTPFANGPSD TPEEILTRIG SGKFTLSGGN WNTVSETAKD LVSKMLHVDP HQRLTAKQVL QHPWVTQKDK LPQSQLSHQD LQLVKGAMAA TYSALNSSKP TPQLKPIESS ILAQRRVRKL PSTTL |
| 预测分子量 | 82,7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为3篇关于RPS6KA1重组蛋白的模拟参考文献示例(注：内容为示例性概括，非真实文献)：
1. **文献名称**: "Recombinant RPS6KA1 Kinase Production and Its Role in MAPK/ERK Signaling"
**作者**: Thompson L, et al.
**摘要**: 研究报道了通过大肠杆菌系统高效表达并纯化重组人源RPS6KA1蛋白，验证其激酶活性可通过磷酸化下游转录因子(如CREB)调控MAPK/ERK信号通路，提示其在细胞增殖中的潜在作用。
2. **文献名称**: "Structural Analysis of RPS6KA1 Reveals Substrate Recognition Mechanisms"
**作者**: Zhang Y, et al.
**摘要**: 通过X射线晶体学解析重组RPS6KA1的催化结构域三维结构，揭示了其与ATP类似物及底物肽段的结合模式，为设计选择性激酶抑制剂提供结构基础。
3. **文献名称**: "RPS6KA1 Recombinant Protein Enhances Neuronal Survival in Oxidative Stress Models"
**作者**: Costa M, et al.
**摘要**: 利用哺乳动物细胞表达的重组RPS6KA1蛋白处理神经元细胞，发现其通过激活mTORC1通路减少氧化应激诱导的凋亡，表明其在神经退行性疾病中的保护性功能。
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如需真实文献，建议通过PubMed或Web of Science检索关键词 "RPS6KA1 recombinant protein" 或 "p90RSK1 expression"。
**Background of RPS6Ka1 Recombinant Protein**
RPS6Ka1 (Ribosomal Protein S6 Kinase A1), also known as p90RSK1 or MAPKAPK1A, is a serine/threonine kinase belonging to the AGC kinase family. It is a downstream effector of the Ras/MAPK (mitogen-activated protein kinase) signaling pathway, primarily activated by ERK1/2 (extracellular signal-regulated kinases 1/2) through phosphorylation. RPS6Ka1 plays a critical role in regulating cell growth, survival, and differentiation by modulating transcription, translation, and cytoskeletal dynamics. Its targets include transcription factors (e.g., CREB, c-Fos), apoptotic regulators (e.g., BAD), and other signaling molecules, linking extracellular stimuli to genomic and metabolic responses.
The protein contains two functional kinase domains: an N-terminal kinase domain responsible for substrate phosphorylation and a C-terminal domain regulating its own activation. Dysregulation of RPS6Ka1 has been implicated in cancers (e.g., breast, ovarian), where it promotes tumor progression, metastasis, and therapy resistance. It also contributes to neurological processes, including synaptic plasticity and memory formation, with potential ties to neurodegenerative diseases.
Recombinant RPS6Ka1 is produced using heterologous expression systems (e.g., *E. coli*, mammalian cells) for biochemical studies, drug discovery, and functional assays. Its purified form enables research on kinase-substrate interactions, pathway crosstalk, and inhibitor screening. As a therapeutic target, RPS6Ka1 is explored for cancer and neurological disorders, highlighting its dual role in health and disease.
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