| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PMSA |
| Uniprot No | Q04609 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 44-750aa |
| 氨基酸序列 | KSSNEATNITPKHNMKAFLDELKAENIKKFLYNFTQIPHLAGTEQNFQLA KQIQSQWKEFGLDSVELAHYDVLLSYPNKTHPNYISIINEDGNEIFNTSL FEPPPPGYENVSDIVPPFSAFSPQGMPEGDLVYVNYARTEDFFKLERDMK INCSGKIVIARYGKVFRGNKVKNAQLAGAKGVILYSDPADYFAPGVKSYP DGWNLPGGGVQRGNILNLNGAGDPLTPGYPANEYAYRRGIAEAVGLPSIP VHPIGYYDAQKLLEKMGGSAPPDSSWRGSLKVPYNVGPGFTGNFSTQKVK MHIHSTNEVTRIYNVIGTLRGAVEPDRYVILGGHRDSWVFGGIDPQSGAA VVHEIVRSFGTLKKEGWRPRRTILFASWDAEEFGLLGSTEWAEENSRLLQ ERGVAYINADSSIEGNYTLRVDCTPLMYSLVHNLTKELKSPDEGFEGKSL YESWTKKSPSPEFSGMPRISKLGSGNDFEVFFQRLGIASGRARYTKNWET NKFSGYPLYHSVYETYELVEKFYDPMFKYHLTVAQVRGGMVFELANSIVL PFDCRDYAVVLRKYADKIYSISMKHPQEMKTYSVSFDSLFSAVKNFTEIA SKFSERLQDFDKSNPIVLRMMNDQLMFLERAFIDPLGLPDRPFYRHVIYA PSSHNKYAGESFPGIYDALFDIESKVDPSKAWGEVKRQIYVAAFTVQAAA ETLSEVA |
| 预测分子量 | 80 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PMSA(前列腺特异性膜抗原)重组蛋白研究的示例参考文献(注：以下内容为示例性概括，具体文献需通过学术数据库检索验证)：
---
1. **《Recombinant PMSA protein production and its application in prostate cancer diagnosis》**
- 作者：Smith J, et al.
- 摘要：研究通过大肠杆菌表达系统成功表达并纯化重组PMSA蛋白，验证其抗原性可用于前列腺癌血清学检测试剂的开发。
2. **《Engineering of PMSA-specific monoclonal antibodies using recombinant protein epitopes》**
- 作者：Lee C, et al.
- 摘要：利用重组PMSA蛋白筛选高亲和力单克隆抗体，证明其在靶向治疗和肿瘤影像学中的潜在应用价值。
3. **《Structural and functional characterization of PMSA extracellular domain》**
- 作者：Zhang Y, et al.
- 摘要：解析重组PMSA胞外区晶体结构，揭示其与抗体结合的分子机制，为设计基于PMSA的免疫疗法提供结构基础。
4. **《Recombinant PMSA nanoparticles for targeted drug delivery in prostate cancer》**
- 作者：Wang H, et al.
- 摘要：开发基于重组PMSA蛋白的纳米颗粒递送系统，验证其在体内外模型中特异性靶向肿瘤细胞并增强化疗效果。
---
建议通过PubMed、Web of Science或Google Scholar以关键词“recombinant PMSA protein”检索最新文献，重点关注其在癌症诊断、靶向治疗及免疫机制中的研究进展。
**Background of PMSA Recombinant Protein**
Prostate-specific membrane antigen (PMSA), also known as folate hydrolase 1 (FOLH1) or glutamate carboxypeptidase II, is a type II transmembrane glycoprotein predominantly expressed in prostate epithelial cells. It is encoded by the *FOLH1* gene and consists of three domains: an intracellular N-terminal domain, a transmembrane region, and a large extracellular enzymatic domain. PMSA functions as a glutamate-preferring carboxypeptidase, playing roles in nutrient absorption, folate metabolism, and neuropeptide processing.
While PMSA is expressed at low levels in normal tissues, its overexpression is strongly associated with prostate cancer progression, particularly in advanced, metastatic, and hormone-refractory cases. This tumor-specific expression profile has made PMSA a prime molecular target for diagnostic and therapeutic applications.
Recombinant PMSA protein is engineered using expression systems like mammalian cells (e.g., HEK293 or CHO) or *E. coli* to ensure proper folding and post-translational modifications. The purified protein retains enzymatic activity and antigenicity, enabling its use in developing PMSA-targeted therapies, such as antibody-drug conjugates (e.g., lutetium-177-PSMA-617), CAR-T cell therapies, and vaccines. It also serves as a critical reagent for diagnostic assays (e.g., PSMA-PET imaging) and antibody validation.
Research on PMSA recombinant protein has advanced precision oncology, offering tools to study tumor biology, drug resistance, and biomarker discovery. Its clinical translation highlights the growing importance of recombinant proteins in bridging molecular biology and personalized cancer treatment.
×
