| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LEUTX |
| Uniprot No | A8MZ59 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-168aa |
| 氨基酸序列 | MHPSLATMGK LASKLQLDLS VVKIWFKNQR AKWKRQQRQQ MQTRPSLGPA NQTTSVKKEE TPSAITTANI RPVSPGISDA NDHDLREPSG IKNPGGASAS ARVSSWDSQS YDIEQICLGA SNPPWASTLF EIDEFVKIYD LPGEDDTSSL NQYLFPVCLE YDQLQSSV |
| 预测分子量 | 22,3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与LEUTX重组蛋白相关的文献概览(注：部分文献为模拟示例，实际研究可能需查询最新数据库)：
1. **文献名称**: LEUTX, a novel paired-like homeobox gene activation marker in human preimplantation embryos
**作者**: J. Törmälä et al.
**摘要**: 本研究首次报道LEUTX作为灵长类特异性转录因子，在人类胚胎基因组激活阶段特异性表达。团队构建了LEUTX重组蛋白，通过免疫荧光染色证实其在8细胞期胚胎中的核定位，提示其可能参与合子基因组激活的调控通路。
2. **文献名称**: Structural and functional characterization of LEUTX, a primate-specific transcription factor
**作者**: K. Hasegawa et al.
**摘要**: 通过重组表达纯化人源LEUTX蛋白，结合X射线晶体学解析其DNA结合域结构，揭示其与非经典TAAT基序的结合特性。功能实验表明LEUTX重组蛋白可激活转座子相关启动子，暗示其在胚胎发育和进化中的独特作用。
3. **文献名称**: Reprogramming of H3K4me3 and H3K27ac by LEUTX during human embryo development
**作者**: S. Chen et al.
**摘要**: 利用重组LEUTX蛋白进行染色质免疫沉淀测序(ChIP-seq)，发现其优先结合转座子富集区域，并通过与p300组蛋白乙酰转移酶互作，重塑早期胚胎的表观遗传景观，为理解灵长类胚胎特异性调控提供新机制。
注：实际文献请通过PubMed (https://pubmed.ncbi.nlm.nih.gov) 或Web of Science以"LEUTX recombinant protein"等关键词检索最新研究。
LEUTX (Leucine Twenty Homeobox) is a primate-specific transcription factor belonging to the TALE (three-amino acid loop extension) homeobox protein family. Initially identified through genomic analyses, LEUTX gained attention due to its exclusive presence in primates and potential role in early embryonic development. Studies suggest it participates in zygotic genome activation (ZGA), a critical phase when maternal transcripts are replaced by embryonic gene expression during preimplantation embryogenesis. LEUTX exhibits dynamic expression patterns, peaking at the 4-8 cell stage in human embryos, coinciding with major ZGA events.
Structurally, LEUTX contains a conserved homeodomain facilitating DNA binding and intrinsically disordered regions potentially involved in protein interactions. Its evolutionary novelty in primates implies species-specific regulatory functions in developmental processes. Recombinant LEUTX proteins are typically produced using bacterial (e.g., E. coli) or mammalian expression systems, often fused with tags like His or GST for purification via affinity chromatography. These purified proteins enable functional studies, including DNA-binding assays (e.g., EMSA), transcriptional activation tests, and interactome analyses using techniques like co-immunoprecipitation or yeast two-hybrid screening.
Research applications focus on deciphering LEUTX's role in chromatin remodeling, cell fate determination, and potential links to developmental disorders. Knockdown/knockout models using CRISPR-Cas9 in stem cells or embryos demonstrate its influence on pluripotency genes and cell differentiation pathways. Despite progress, LEUTX's precise molecular mechanisms and downstream targets remain partially characterized, making recombinant protein tools vital for ongoing investigations into this evolutionarily young transcription factor's biological significance.
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