| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | FITC |
| Uniprot No | Q2FXS9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-106aa |
| 氨基酸序列 | MITVDITVNDEGKVTDVIMDGHADHGEYGHDIVCAGASAVLFGSVNAIIGLTSERPDINYDDNGGHFHIRSVDTNNDEAQLILQTMLVSLQTIEEEYNENIRLNYK |
| 预测分子量 | 11,6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于FITC重组蛋白的参考文献示例(注：以下内容为学术场景模拟，非真实文献)：
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1. **《Fluorescent Labeling of Recombinant Proteins with FITC for Live-Cell Imaging》**
*作者：Chen L, et al.*
摘要：提出一种温和的FITC标记重组蛋白方法，利用His标签与镍离子螯合物定向结合，实现高标记效率(>90%)，并验证其在活细胞动态追踪中的应用效果。
2. **《Optimization of FITC-Conjugated Antibody Production Using Recombinant Protein Technology》**
*作者：Sato K, et al.*
摘要：通过基因工程将半胱氨酸引入抗体Fc区，实现FITC定点偶联，减少功能域干扰，提升肿瘤靶向荧光成像的信噪比，适用于体外诊断试剂开发。
3. **《FITC-Labeled Recombinant Lectin as a Probe for Glycan Analysis on Cell Surfaces》**
*作者：Müller R, et al.*
摘要：构建FITC标记的重组植物凝集素蛋白，用于流式细胞术检测癌细胞表面聚糖表达差异，证实其特异性结合甘露糖残基的能力及临床筛查潜力。
4. **《A Novel FITC-Recombinant Protein Nanoparticle for Drug Delivery Tracking》**
*作者：Wang Y, et al.*
摘要：开发基于白蛋白重组蛋白的FITC-纳米载体，通过小鼠模型验证其可通过荧光信号实时监测药物在肝脏和肿瘤组织的递送分布动力学。
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提示：实际文献需通过PubMed/Google Scholar检索关键词"FITC recombinant protein"+"fluorescent labeling"获取。
Fluorescein isothiocyanate (FITC)-conjugated recombinant proteins are engineered biomolecules widely used as fluorescent probes in biomedical research and diagnostics. Recombinant proteins are produced through genetic engineering, where target proteins are expressed in host systems (e.g., E. coli, mammalian cells) with precise control over sequence and post-translational modifications. FITC, a derivative of fluorescein, is a small organic fluorophore that emits green fluorescence (~520 nm) when excited by blue light (~495 nm). Its isothiocyanate group enables covalent conjugation to primary amines (-NH₂) in proteins, typically under mild alkaline conditions, forming stable thiourea bonds.
The combination of recombinant protein technology with FITC labeling merges molecular specificity with optical detectability. Recombinant proteins retain their native structure and function after labeling, allowing applications in tracking protein localization, receptor-ligand interactions, and intracellular trafficking. FITC’s high quantum yield and photostability make it suitable for techniques like flow cytometry, fluorescence microscopy, and ELISA. However, FITC has limitations, including pH sensitivity (fluorescence quenches below pH 8) and susceptibility to photobleaching.
These labeled proteins are particularly valuable in immunology (e.g., antibody detection), cell biology (live-cell imaging), and drug development (high-throughput screening). Commercial FITC-conjugated recombinant proteins often undergo rigorous quality control to ensure consistent labeling ratios and minimal batch variability. Alternatives like site-specific labeling (e.g., using unnatural amino acids) are emerging, but FITC remains popular due to its cost-effectiveness and established protocols. Proper storage (light-protected, -20°C) is critical to maintain fluorescence intensity and protein stability over time.
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