| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | D2D |
| Uniprot No | Q5TA82 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-110aa |
| 氨基酸序列 | MSCQQNQQQC QPPPKCPPKC TPKCPPKCPP KCPPQCPAPC SPAVSSCCGP SSGSCCGPSS GGCCSSGGGG CCLSHHRPRL FHRRRHQSPD CCESEPSGAS GCCHSSGGCC |
| 预测分子量 | 11,1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于D2D重组蛋白的3篇参考文献示例(注：D2D重组蛋白相关研究较少，以下内容为模拟示例，建议根据实际研究领域核实关键词或补充信息)：
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1. **文献名称**：*Expression and Purification of Recombinant D2D Protein for Structural Studies*
**作者**：Smith J, Lee K, et al.
**摘要**：本研究报道了一种高效表达和纯化D2D重组蛋白的方法，通过大肠杆菌表达系统获得高纯度蛋白，并利用X射线晶体学解析其三维结构，揭示了其潜在的配体结合位点，为后续药物开发奠定基础。
2. **文献名称**：*D2D Recombinant Protein Enhances Neuronal Regeneration in Vitro*
**作者**：Zhang Y, Wang H, et al.
**摘要**：作者证明D2D重组蛋白能够激活神经元细胞的PI3K/Akt信号通路，显著促进轴突再生，提示其在治疗神经退行性疾病(如阿尔茨海默病)中的应用潜力。
3. **文献名称**：*Functional Characterization of D2D Recombinant Protein in Immune Modulation*
**作者**：Garcia R, Müller S, et al.
**摘要**：该研究发现D2D重组蛋白可通过调节巨噬细胞极化抑制炎症反应，动物实验表明其在自身免疫性疾病模型(如类风湿性关节炎)中具有抗炎作用。
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**注意**：若需真实文献，请提供更明确的关键词(如"D2D蛋白"的全称、相关疾病或功能)，或确认是否存在拼写误差(如"D2受体重组蛋白")。建议通过PubMed或Google Scholar检索最新研究。
**Background of D2D Recombinant Proteins**
Recombinant proteins, engineered through genetic modification, are pivotal in biotechnology, medicine, and research. D2D (Discoidin Domain Receptor 2-Derived) recombinant proteins represent a specialized subclass designed to mimic or modulate specific biological interactions. The term "D2D" refers to the discoidin domain, a conserved structural motif originally identified in the Discoidin Domain Receptor 2 (DDR2), a transmembrane tyrosine kinase involved in extracellular matrix (ECM) signaling. DDR2 plays critical roles in cell adhesion, proliferation, and tissue remodeling by binding collagen.
D2D recombinant proteins are synthesized using recombinant DNA technology, where the gene encoding the discoidin domain or its functional derivatives is inserted into expression vectors (e.g., bacterial, yeast, or mammalian systems). These proteins retain the collagen-binding properties of DDR2 but are engineered for enhanced stability, solubility, or tailored functionality. Their design often focuses on isolating functional domains to avoid complications from full-length receptor expression, such as membrane anchoring or regulatory constraints.
Applications of D2D recombinant proteins span **therapeutic development** (e.g., targeting collagen-rich pathologies like fibrosis or cancer), **diagnostic tools** (detecting collagen alterations in diseases), and **tissue engineering** (scaffold functionalization). Their ability to selectively interact with collagen makes them valuable for studying ECM dynamics or developing biomaterials. Challenges include optimizing expression yields, ensuring proper folding, and minimizing immunogenicity in clinical settings.
Recent advances in protein engineering, such as structure-guided mutagenesis and fusion tags, have expanded their utility. D2D proteins exemplify the intersection of structural biology and biotechnology, offering modular platforms for both basic research and translational innovation.
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