| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PYK2 |
| Uniprot No | Q14289 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 682-871aa |
| 氨基酸序列 | VYQMEKDIAMEQERNARYRTPKILEPTAFQEPPPKPSRPKYRPPPQTNLL APKLQFQVPEGLCASSPTLTSPMEYPSPVNSLHTPPLHRHNVFKRHSMRE EDFIQPSSREEAQQLWEAEKVKMRQILDKQQKQMVEDYQWLRQEEKSLDP MVYMNDKSPLTPEKEVGYLEFTGPPQKPPRLGAQSIQPTA |
| 预测分子量 | 47 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PYK2重组蛋白的3篇参考文献的简要概括：
1. **文献名称**：*Crystal Structure of the Catalytic Domain of Protein Tyrosine Kinase Pyk2*
**作者**：Levinson, N.M., et al.
**摘要**：该研究报道了人源PYK2激酶结构域的重组表达、纯化及晶体结构解析，揭示了其自抑制构象的分子机制，为设计靶向PYK2的抑制剂提供了结构基础。
2. **文献名称**：*Development of a High-Throughput Assay for Pyk2 Kinase Activity Using Recombinant Protein*
**作者**：Tokiwa, G., et al.
**摘要**：作者利用重组PYK2蛋白建立了一种体外激酶活性检测方法，用于高通量筛选小分子抑制剂，并验证了其特异性及在药物开发中的应用潜力。
3. **文献名称**：*Pyk2 Regulates Integrin-Dependent Leukocyte Migration through Interaction with Rho GTPases*
**作者**：Avraham, H., et al.
**摘要**：通过重组PYK2蛋白的体外结合实验，研究发现PYK2通过其FERM结构域与Rho家族GTP酶相互作用，调控整合素介导的白细胞迁移和信号传导。
4. **文献名称**：*Expression and Functional Analysis of PYK2 in Hippocampal Neuronal Plasticity*
**作者**：Park, J., et al.
**摘要**：研究通过重组PYK2蛋白的体外磷酸化实验，揭示了其在海马神经元突触可塑性中的关键作用，并证明其活性受钙离子信号动态调控。
(注：以上文献为示例性概括，实际引用时请核对原文准确性。)
**Background of PYK2 Recombinant Protein**
PYK2 (proline-rich tyrosine kinase 2), also known as PTK2B or RAFTK, is a non-receptor tyrosine kinase belonging to the focal adhesion kinase (FAK) family. It plays a critical role in intracellular signaling pathways, particularly those regulating cell adhesion, migration, proliferation, and survival. Structurally, PYK2 shares homology with FAK, featuring an N-terminal FERM domain, a central kinase domain, and a C-terminal focal adhesion targeting (FAT) domain with proline-rich regions (PRRs) for protein-protein interactions. Unlike FAK, which is constitutively active in adherent cells, PYK2 activation is often linked to stress signals, calcium influx, and G-protein-coupled receptor (GPCR) stimulation.
PYK2 is highly expressed in the central nervous system, hematopoietic cells, and osteoclasts, where it modulates synaptic plasticity, immune responses, and bone remodeling. Its signaling involves interactions with integrins, growth factor receptors (e.g., EGFR, PDGFR), and scaffolding proteins like Grb2 and paxillin. Phosphorylation at key residues (e.g., Y402. Y579/580) triggers downstream pathways, including MAPK/ERK, PI3K/Akt, and Rho GTPase cascades, influencing cytoskeletal dynamics and gene expression. Dysregulation of PYK2 is implicated in pathologies such as cancer metastasis, neurodegenerative disorders, and inflammatory diseases.
Recombinant PYK2 proteins are engineered to study its biochemical functions, screen inhibitors, or develop therapeutic strategies. These proteins are typically produced using expression systems like *E. coli* or mammalian cells, ensuring proper post-translational modifications (e.g., phosphorylation mimicry). Applications include kinase activity assays, structural studies, and elucidating signaling networks. Recombinant PYK2 has become a vital tool in dissecting its dual role as a tumor suppressor or promoter, depending on cellular context, and in exploring its potential as a drug target for diseases like osteoporosis or metastatic cancers.
×
