| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MOTS-c |
| Uniprot No | A0A0C5B5G6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-16aa |
| 氨基酸序列 | MRWQEMGYIFYPRKLR |
| 预测分子量 | 2,1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MOTS-c重组蛋白的参考文献示例，基于已有研究主题的合理推测，具体文献需通过学术数据库进一步验证：
1. **"Recombinant MOTS-c attenuates metabolic dysfunction via AMPK activation in high-fat diet-induced obesity models"**
*Lee, C., et al. (2018)*
摘要：研究重组MOTS-c在肥胖小鼠模型中的作用，发现其通过激活AMPK通路改善胰岛素抵抗和肝脏脂肪代谢。
2. **"Efficient production and purification of MOTS-c recombinant protein in Escherichia coli for therapeutic applications"**
*Zhang, Y., et al. (2020)*
摘要：描述利用大肠杆菌表达系统高效制备重组MOTS-c蛋白的方法，并验证其体外促进葡萄糖摄取的生物活性。
3. **"MOTS-c recombinant peptide protects against skeletal muscle atrophy through mitochondrial regulation"**
*Reynolds, J.C., et al. (2021)*
摘要：探讨重组MOTS-c通过调节线粒体功能抑制肌肉萎缩，在衰老和疾病模型中的潜在治疗价值。
4. **"Systemic administration of recombinant MOTS-c enhances exercise capacity and metabolic homeostasis in mice"**
*Kim, K.H., et al. (2019)*
摘要：证明重组MOTS-c通过提高能量代谢效率和氧化磷酸化，显著增强小鼠运动耐力并改善代谢指标。
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**注**：以上文献为示例性概括，实际引用时建议通过PubMed、Google Scholar等平台以关键词“MOTS-c recombinant”检索最新研究。
MOTS-c (Mitochondrial Open Reading Frame of the 12S rRNA-c) is a mitochondrial-derived peptide encoded by the mitochondrial DNA’s 12S rRNA region. Discovered in 2015. this 16-amino-acid peptide has emerged as a key regulator of metabolic homeostasis, linking mitochondrial function to systemic energy balance. Unlike traditional nuclear DNA-encoded hormones, MOTS-c represents a novel class of cell signaling molecules derived from mitochondrial DNA, challenging the conventional view of mitochondria solely as energy-producing organelles. It is primarily expressed in skeletal muscle and circulates in the bloodstream, exerting endocrine-like effects on various tissues.
Research has shown MOTS-c plays a critical role in glucose metabolism and insulin sensitivity. It activates the AMP-activated protein kinase (AMPK) pathway, enhancing cellular glucose uptake and improving metabolic flexibility under stress conditions. Notably, its levels decline with age, and preclinical studies suggest its potential in mitigating age-related metabolic dysfunction, obesity, and insulin resistance. Animal models demonstrate that MOTS-c administration reduces fat accumulation, improves exercise capacity, and extends healthspan, positioning it as a promising therapeutic target for metabolic disorders.
Recombinant MOTS-c production utilizes genetic engineering to express the peptide in bacterial or mammalian systems, enabling scalable synthesis for research and clinical applications. Challenges include maintaining peptide stability and ensuring proper post-translational modifications critical for bioactivity. Current studies focus on optimizing delivery methods and evaluating long-term safety. As a naturally occurring mitochondrial signal, MOTS-c offers unique insights into the cross-talk between mitochondria and systemic metabolism, with implications for developing novel treatments for diabetes, obesity, and age-related diseases.
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