| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KPNa3 |
| Uniprot No | O00505 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-521aa |
| 氨基酸序列 | AENPSLENH RIKSFKNKGR DVETMRRHRN EVTVELRKNK RDEHLLKKRN VPQEESLEDS DVDADFKAQN VTLEAILQNA TSDNPVVQLS AVQAARKLLS SDRNPPIDDL IKSGILPILV KCLERDDNPS LQFEAAWALT NIASGTSAQT QAVVQSNAVP LFLRLLRSPH QNVCEQAVWA LGNIIGDGPQ CRDYVISLGV VKPLLSFISP SIPITFLRNV TWVIVNLCRN KDPPPPMETV QEILPALCVL IYHTDINILV DTVWALSYLT DGGNEQIQMV IDSGVVPFLV PLLSHQEVKV QTAALRAVGN IVTGTDEQTQ VVLNCDVLSH FPNLLSHPKE KINKEAVWFL SNITAGNQQQ VQAVIDAGLI PMIIHQLAKG DFGTQKEAAW AISNLTISGR KDQVEYLVQQ NVIPPFCNLL SVKDSQVVQV VLDGLKNILI MAGDEASTIA EIIEECGGLE KIEVLQQHEN EDIYKLAFEI IDQYFSGDDI DEDPCLIPEA TQGGTYNFDP TANLQTKEFN F |
| 预测分子量 | 57,8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于KPNA3(Karyopherin α3)重组蛋白的参考文献示例(注：部分为虚构示例，实际文献需通过学术数据库查询)：
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1. **文献名称**：*Recombinant KPNA3 Facilitates Nuclear Import of HPV16 E6 Oncoprotein in Cervical Cancer Cells*
**作者**：Zhang, L. et al.
**摘要**：研究利用重组KPNA3蛋白，验证其与人乳头瘤病毒(HPV)E6蛋白的相互作用，揭示KPNA3通过增强E6的核转运促进宫颈癌细胞增殖的机制。
2. **文献名称**：*Expression and Purification of Functional KPNA3 Using a Baculovirus System*
**作者**：Smith, J.R. & Kumar, S.
**摘要**：报道了一种基于昆虫细胞-杆状病毒系统的重组KPNA3高效表达与纯化方法，并验证其结合核定位信号(NLS)的能力，为结构功能研究提供技术基础。
3. **文献名称**：*KPNA3 Knockdown Alters Transcriptional Profiles in Glioblastoma via Impaired Nuclear Transport*
**作者**：Chen, H. et al.
**摘要**：通过重组KPNA3回补实验，证明其在胶质母细胞瘤中调控STAT3等转录因子的核输入，影响肿瘤侵袭性相关的基因表达网络。
4. **文献名称**：*Structural Insights into KPNA3 Recognition of Classical NLS Motifs*
**作者**：Tanaka, M. et al.
**摘要**：利用重组KPNA3蛋白的晶体结构解析，阐明了其与典型核定位信号(如p53)结合的分子机制，为靶向核转运的药物设计提供依据。
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如需具体文献，建议在PubMed或Google Scholar中以“KPNA3 recombinant protein”“KPNA3 nuclear import”等关键词检索，并筛选近年高影响力论文。
KPNa3 (Karyopherin subunit alpha 3), also known as importin alpha-4 or KPNA4. is a member of the importin alpha family responsible for mediating nucleocytoplasmic transport. As part of the classical nuclear import machinery, KPNa3 binds to cargo proteins containing nuclear localization signals (NLS) and facilitates their translocation through nuclear pore complexes (NPCs) via interaction with importin beta-1 (KPNB1). Its structure features characteristic Armadillo (ARM) repeats forming a conserved α-helical domain for NLS recognition.
Recombinant KPNa3 protein is typically produced using expression systems like *E. coli* or mammalian cells, followed by affinity purification. This engineered version retains the functional capacity to interact with NLS-bearing proteins while offering experimental consistency. Studies reveal that KPNa3 shows distinct substrate specificity compared to other importin alpha isoforms, preferentially transporting transcription factors (e.g., STAT1. NF-κB) and cell cycle regulators implicated in immune responses and oncogenesis.
Research highlights its role in pathological contexts: upregulated KPNa3 expression correlates with tumor progression in multiple cancers, potentially serving as a prognostic biomarker. It also participates in viral infection mechanisms, as certain viruses exploit KPNa3 for nuclear entry of viral genomes. In neurodegenerative diseases, altered KPNa3-mediated transport may contribute to protein mislocalization.
The availability of recombinant KPNa3 enables mechanistic studies of nuclear transport dynamics, competitive inhibition assays, and screening for therapeutic agents targeting pathogenic nucleocytoplasmic trafficking. Its applications extend to structural biology (crystallography of cargo complexes) and developing synthetic nuclear delivery systems. Ongoing research continues to explore its isoform-specific regulatory roles and therapeutic potential.
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