| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PURA |
| Uniprot No | Q00577 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-322aa |
| 氨基酸序列 | MADRDSGSEQ GGAALGSGGS LGHPGSGSGS GGGGGGGGGG GGSGGGGGGA PGGLQHETQE LASKRVDIQN KRFYLDVKQN AKGRFLKIAE VGAGGNKSRL TLSMSVAVEF RDYLGDFIEH YAQLGPSQPP DLAQAQDEPR RALKSEFLVR ENRKYYMDLK ENQRGRFLRI RQTVNRGPGL GSTQGQTIAL PAQGLIEFRD ALAKLIDDYG VEEEPAELPE GTSLTVDNKR FFFDVGSNKY GVFMRVSEVK PTYRNSITVP YKVWAKFGHT FCKYSEEMKK IQEKQREKRA ACEQLHQQQQ QQQEETAAAT LLLQGEEEGE ED |
| 预测分子量 | 34,9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于PURA重组蛋白的文献摘要信息，供参考：
1. **文献名称**：*Pur-alpha regulates RhoA developmental expression and downstream signaling*
**作者**：Johnson EM et al.
**摘要**：研究利用重组人PURA蛋白探究其与RhoA启动子的结合能力，发现PURA通过调控RhoA基因表达影响神经元发育和细胞迁移，为神经退行性疾病机制提供新视角。
2. **文献名称**：*Expression and purification of recombinant human PURA protein in E. coli*
**作者**：Wang L et al.
**摘要**：报道在大肠杆菌中高效表达带His标签的PURA重组蛋白，通过镍柱亲和层析纯化，获得高纯度蛋白，验证其与单链DNA结合活性，为后续功能研究奠定基础。
3. **文献名称**：*Structural insights into PURA protein’s DNA unwinding activity*
**作者**：Kumar A & Smith SB
**摘要**：通过冷冻电镜解析重组PURA蛋白与DNA复合物的三维结构，揭示其依赖ATP的DNA解旋酶活性机制，提出该蛋白参与基因组稳定性的分子模型。
注：以上文献信息为示例性综合内容，实际文献需通过PubMed/Google Scholar检索关键词“PURA recombinant protein”或“Pur-alpha expression”获取。如需具体文章，可补充说明研究侧重方向(如表达纯化、神经疾病或结构生物学方向)。
PURA recombinant proteins are derived from the PURA gene, which encodes Pur-alpha, a highly conserved nucleic acid-binding protein involved in diverse cellular processes. Discovered in the early 1990s, Pur-alpha contains three evolutionary conserved repeats that facilitate interactions with DNA, RNA, and protein partners. It plays critical roles in DNA replication, transcriptional regulation, and mRNA transport, particularly in neuronal development and myelination.
The recombinant form is engineered using expression systems like *E. coli* or mammalian cells to produce purified protein for functional studies. PURA's significance stems from its dual role in normal physiology and disease. It regulates cell cycle progression and chromatin remodeling, while dysfunction is linked to neurodevelopmental disorders (e.g., PURA syndrome), cancers, and viral pathogenesis (e.g., HIV-1 replication). Researchers utilize PURA recombinant proteins to investigate its molecular mechanisms, including its ability to bind G-rich sequences in gene promoters or stabilize mRNA complexes.
Recent interest focuses on its therapeutic potential. In neurodegeneration, PURA interacts with proteins implicated in ALS and FXTAS, suggesting targets for intervention. Its role in suppressing oncogene transcription also positions it as a candidate for cancer therapy development. However, challenges remain in understanding tissue-specific isoforms and post-translational modifications affecting its function. Current applications span structural biology studies, drug screening platforms, and gene delivery systems using PURA's DNA-unwinding properties. As a research tool, it continues to illuminate fundamental processes in chromatin dynamics and RNA biology.
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