| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RIPK2 |
| Uniprot No | O43353 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-540aa |
| 氨基酸序列 | MNGEAICSALPTIPYHKLADLRYLSRGASGTVSSARHADWRVQVAVKHLHIHTPLLDSERKDVLREAEILHKARFSYILPILGICNEPEFLGIVTEYMPNGSLNELLHRKTEYPDVAWPLRFRILHEIALGVNYLHNMTPPLLHHDLKTQNILLDNEFHVKIADFGLSKWRMMSLSQSRSSKSAPEGGTIIYMPPENYEPGQKSRASIKHDIYSYAVITWEVLSRKQPFEDVTNPLQIMYSVSQGHRPVINEESLPYDIPHRARMISLIESGWAQNPDERPSFLKCLIELEPVLRTFEEITFLEAVIQLKKTKLQSVSSAIHLCDKKKMELSLNIPVNHGPQEESCGSSQLHENSGSPETSRSLPAPQDNDFLSRKAQDCYFMKLHHCPGNHSWDSTISGSQRAAFCDHKTTPCSSAIINPLSTAGNSERLQPGIAQQWIQSKREDIVNQMTEACLNQSLDALLSRDLIMKEDYELVSTKPTRTSKVRQLLDTTDIQGEEFAKVIVQKLKDNKQMGLQPYPEILVVSRSPSLNLLQNKSM |
| 预测分子量 | 62.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RIPK2重组蛋白的模拟参考文献示例(仅供参考，具体文献需通过学术数据库验证)：
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1. **标题**: *Structural insights into RIPK2 kinase activation and its interaction with NOD1/2*
**作者**: X. Li, J.P. Ting, et al.
**摘要**: 研究通过重组RIPK2蛋白的晶体结构解析，揭示了其激酶结构域的构象变化及与NOD1/2信号通路中关键蛋白的相互作用机制，为靶向RIPK2的抑制剂设计提供依据。
2. **标题**: *RIPK2 ubiquitination regulates inflammatory signaling via TAK1 activation*
**作者**: M. Lamkanfi, V.M. Dixit, et al.
**摘要**: 利用重组RIPK2蛋白进行体外泛素化实验，证明其K63位泛素化修饰在激活TAK1-NF-κB通路中的关键作用，并揭示了RIPK2在先天免疫中的调控网络。
3. **标题**: *Development of a high-throughput assay for RIPK2 kinase activity using recombinant protein*
**作者**: S. Haile, R.J. Ulevitch, et al.
**摘要**: 报道了一种基于重组RIPK2蛋白的激酶活性检测方法，用于筛选小分子抑制剂，并评估其在炎症性疾病治疗中的潜在应用。
4. **标题**: *RIPK2 autophosphorylation and its role in bacterial sensing*
**作者**: G. Nuñez, K.A. Fitzgerald, et al.
**摘要**: 通过重组RIPK2蛋白的功能实验，阐明其自磷酸化位点对NOD2介导的细菌肽聚糖识别的重要性，以及下游促炎细胞因子产生的分子机制。
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**备注**：以上为模拟文献，实际引用请通过PubMed、Web of Science或Google Scholar检索关键词(如“RIPK2 recombinant protein”“RIPK2 kinase activity”)获取真实文献。建议关注近年发表于*Nature Immunology*、*Cell Host & Microbe*等期刊的研究。
Receptor-interacting protein kinase 2 (RIPK2) is a serine/threonine kinase belonging to the RIP kinase family, playing a pivotal role in innate and adaptive immune signaling. It acts as a critical mediator in the NOD1/NOD2 (nucleotide-binding oligomerization domain-containing protein 1/2) pathways, which are cytosolic pattern recognition receptors (PRRs) responsible for detecting bacterial peptidoglycan fragments. Upon ligand recognition, RIPK2 is recruited to form signaling complexes, facilitating the activation of NF-κB and MAPK pathways to induce pro-inflammatory cytokine production. Its kinase activity and scaffolding function are essential for downstream immune responses, linking microbial sensing to inflammatory gene expression.
Recombinant RIPK2 protein is engineered for in vitro studies to dissect its structural, functional, and interaction dynamics. Produced via heterologous expression systems (e.g., E. coli, insect, or mammalian cells), the recombinant form often includes tags (e.g., His, GST) for purification and detection. Researchers utilize it to study kinase activity, screen inhibitors, or map binding partners, aiding drug discovery for inflammatory and autoimmune diseases. Notably, dysregulated RIPK2 signaling is implicated in Crohn’s disease, rheumatoid arthritis, and other inflammatory disorders, making it a therapeutic target. Recent studies also explore its role in cell death pathways and cross-talk with other immune sensors, broadening its relevance in infection and immunity. Despite progress, challenges remain in understanding post-translational modifications (e.g., ubiquitination) that regulate its activation. Recombinant RIPK2 thus serves as a vital tool to unravel mechanistic insights and develop targeted therapies.
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