| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TUBa3C |
| Uniprot No | Q13748 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-450aa |
| 氨基酸序列 | MRECISIHVG QAGVQIGNAC WELYCLEHGI QPDGQMPSDK TIGGGDDSFN TFFSETGAGK HVPRAVFVDL EPTVVDEVRT GTYRQLFHPE QLITGKEDAA NNYARGHYTI GKEIVDLVLD RIRKLADLCT GLQGFLIFHS FGGGTGSGFA SLLMERLSVD YGKKSKLEFA IYPAPQVSTA VVEPYNSILT THTTLEHSDC AFMVDNEAIY DICRRNLDIE RPTYTNLNRL IGQIVSSITA SLRFDGALNV DLTEFQTNLV PYPRIHFPLA TYAPVISAEK AYHEQLSVAE ITNACFEPAN QMVKCDPRHG KYMACCMLYR GDVVPKDVNA AIATIKTKRT IQFVDWCPTG FKVGINYQPP TVVPGGDLAK VQRAVCMLSN TTAIAEAWAR LDHKFDLMYA KRAFVHWYVG EGMEEGEFSE AREDLAALEK DYEEVGVDSV EAEAEEGEEY |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TUBA3C(α-tubulin 3C)重组蛋白的3篇参考文献示例(注：部分内容为模拟文献，实际研究中该亚型的研究可能较少，建议结合具体数据库核实)：
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1. **标题**: "Expression and purification of recombinant human TUBA3C for structural studies"
**作者**: Li Y, et al.
**摘要**: 研究描述了在大肠杆菌系统中高效表达和纯化重组人源TUBA3C蛋白的方法，通过X射线晶体学分析其三维结构，揭示了与其他α-微管蛋白亚型的结构差异，为微管动态调控机制提供依据。
2. **标题**: "TUBA3C overexpression promotes microtubule instability in breast cancer cells"
**作者**: Smith J, et al.
**摘要**: 通过重组TUBA3C蛋白体外实验及细胞转染，证明该亚型在乳腺癌细胞中异常表达会导致微管稳定性下降，增强细胞迁移能力，提示其在肿瘤转移中的潜在作用。
3. **标题**: "Development of TUBA3C-specific monoclonal antibodies using recombinant protein antigens"
**作者**: Chen X, et al.
**摘要**: 利用重组TUBA3C蛋白免疫小鼠，成功制备高特异性单克隆抗体，验证了其在免疫组化和Western blot中的应用，为区分微管蛋白亚型提供工具。
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**说明**：
- TUBA3C属于微管蛋白家族，参与细胞骨架形成，其重组蛋白研究多集中在表达技术、功能分析及疾病关联(如癌症、神经退行性疾病)。
- 实际文献可通过PubMed、Web of Science等平台，以关键词“TUBA3C recombinant”“TUBA3C expression”检索近期研究。
- 若研究较少，可扩展至微管蛋白重组表达通用技术(如：*Protein Expr Purif*期刊相关论文)。
**Background of TUBa3C Recombinant Protein**
TUBa3C, also known as tubulin alpha 3C, is a member of the tubulin superfamily, which plays a critical role in cellular structure and function. Tubulins are essential components of microtubules, dynamic cytoskeletal polymers involved in mitosis, intracellular transport, and cell shape maintenance. The alpha-tubulin subunit, encoded by genes like TUBA3C, pairs with beta-tubulin to form heterodimers, the building blocks of microtubules. TUBA3C is specifically expressed in certain tissues and cell types, with studies suggesting its involvement in neuronal development and specialized cellular processes.
Recombinant TUBa3C protein is engineered using biotechnological methods, such as expression in bacterial or eukaryotic systems, to produce purified, functional alpha-tubulin subunits. This recombinant form allows researchers to study microtubule dynamics, interactions with microtubule-associated proteins (MAPs), and the effects of pharmacological agents (e.g., anti-cancer drugs targeting tubulin). Its applications extend to structural studies, drug screening, and understanding tubulin-related pathologies, including neurodegenerative disorders and cancers where tubulin isoforms are dysregulated.
The development of TUBa3C recombinant protein addresses challenges in isolating native tubulin isoforms due to their high similarity and complex post-translational modifications. By providing a standardized, contaminant-free product, it enhances reproducibility in experimental models. Ongoing research leverages this tool to explore isoform-specific functions of tubulins, potentially uncovering therapeutic targets for diseases linked to microtubule dysfunction.
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