| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PITPNb |
| Uniprot No | P48739 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-271aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMVLIKEFRVVLPCSVQEYQVGQLYSVAEAS KNETGGGEGIEVLKNEPYEKDGEKGQYTHKIYHLKSKVPAFVRMIAPEGS LVFHEKAWNAYPYCRTIVTNEYMKDDFFIKIETWHKPDLGTLENVHGLDP NTWKTVEIVHIDIADRSQVEPADYKADEDPALFQSVKTKRGPLGPNWKKE LANSPDCPQMCAYKLVTIKFKWWGLQSKVENFIQKQEKRIFTNFHRQLFC WIDKWIDLTMEDIRRMEDETQKELETMRKRGSVRGTSAADV |
| 预测分子量 | 34 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PITPNb重组蛋白的虚构参考文献示例，涵盖不同研究方向：
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1. **标题**: *Structural Insights into Human PITPNb Reveal Mechanisms of Lipid Binding*
**作者**: Zhang L, et al.
**摘要**: 本研究通过X射线晶体学解析了重组人源PITPNb的三维结构，揭示了其与磷脂酰肌醇(PI)结合的关键结构域。实验表明，重组蛋白在体外可有效转运PI，并依赖特定的疏水口袋维持脂质转移活性，为理解其细胞功能提供结构基础。
2. **标题**: *PITPNb Regulates ER-Golgi Trafficking via Phosphoinositide Signaling*
**作者**: Müller S, et al.
**摘要**: 通过基因敲除和重组蛋白回补实验，证明PITPNb通过调控高尔基体PI4P水平影响囊泡运输。重组PITPNb表达可挽救PI4P失衡导致的分泌通路缺陷，提示其在细胞器间信号传导中的核心作用。
3. **标题**: *Recombinant PITPNb Attenuates Hepatic Steatosis in Mouse Models*
**作者**: Kim J, et al.
**摘要**: 利用腺病毒载体在非酒精性脂肪肝小鼠模型中过表达重组PITPNb，发现其通过促进肝细胞PI代谢减少脂质蓄积。研究提示PITPNb可能成为代谢性疾病的新型治疗靶点。
4. **标题**: *High-Yield Expression and Purification of Functional PITPNb in E. coli*
**作者**: Patel R, et al.
**摘要**: 开发了基于大肠杆菌的重组PITPNb高效表达系统，优化纯化步骤后获得高纯度蛋白。生物物理分析证实重组蛋白具有天然构象及脂质转移活性，为后续研究提供可靠工具。
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*注：上述文献为示例性虚构内容，实际研究中需查询真实数据库(如PubMed)获取权威信息。*
PITPNb (phosphatidylinositol transfer protein beta), also known as RdgBβ or PITPNβ, is a member of the phosphatidylinositol transfer protein (PITP) family. These proteins facilitate the transfer of phospholipids, particularly phosphatidylinositol (PI) and phosphatidylcholine (PC), between membrane compartments, playing critical roles in lipid metabolism and cellular signaling. PITPNb is characterized by its N-terminal PITP domain, which binds and transfers lipids, and a C-terminal region containing a Golgi-targeting domain. Unlike its homolog PITPNα, PITPNb localizes predominantly to the trans-Golgi network and endosomal membranes, where it regulates membrane trafficking, organelle dynamics, and lipid homeostasis.
Recombinant PITPNb protein is engineered for in vitro studies to dissect its molecular functions. It is expressed in heterologous systems (e.g., *E. coli* or mammalian cells) and purified to investigate lipid transfer mechanisms, interactions with signaling pathways (e.g., Wnt/β-catenin), and roles in diseases. PITPNb has been implicated in cancer progression, neurodegenerative disorders, and retinal degeneration. For instance, its dysregulation affects PI4P metabolism, influencing secretory pathways and growth signaling in tumors. In neurobiology, PITPNb supports synaptic vesicle recycling and neuronal survival, with links to Alzheimer’s disease pathology.
Research on recombinant PITPNb also explores its therapeutic potential. By modulating lipid signaling or restoring membrane integrity, it may offer strategies for targeting lipid dysregulation-related diseases. Studies in model organisms, such as *Drosophila* (where PITPNb homologs regulate phototransduction), highlight its evolutionary conservation and functional significance. Overall, recombinant PITPNb serves as a vital tool for unraveling lipid-mediated cellular processes and developing biomedical interventions.
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