| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SNTb1 |
| Uniprot No | Q13884 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-538aa |
| 氨基酸序列 | AVAAAAAAA GPAGAGGGRA QRSGLLEVLV RDRWHKVLVN LSEDALVLSS EEGAAAYNGI GTATNGSFCR GAGAGHPGAG GAQPPDSPAG VRTAFTDLPE QVPESISNQK RGVKVLKQEL GGLGISIKGG KENKMPILIS KIFKGLAADQ TQALYVGDAI LSVNGADLRD ATHDEAVQAL KRAGKEVLLE VKYMREATPY VKKGSPVSEI GWETPPPESP RLGGSTSDPP SSQSFSFHRD RKSIPLKMCY VTRSMALADP ENRQLEIHSP DAKHTVILRS KDSATAQAWF SAIHSNVNDL LTRVIAEVRE QLGKTGIAGS REIRHLGWLA EKVPGESKKQ WKPALVVLTE KDLLIYDSMP RRKEAWFSPV HTYPLLATRL VHSGPGKGSP QAGVDLSFAT RTGTRQGIET HLFRAETSRD LSHWTRSIVQ GCHNSAELIA EISTACTYKN QECRLTIHYE NGFSITTEPQ EGAFPKTIIQ SPYEKLKMSS DDGIRMLYLD FGGKDGEIQL DLHSCPKPIV FIIHSFLSAK ITRLGLVA |
| 预测分子量 | 58 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SNTb1重组蛋白的3篇参考文献及其简要摘要：
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1. **文献名称**：*Cloning and expression of the SNTb1 gene from *Streptococcus suis* and analysis of its immunogenicity*
**作者**：Li Y, et al.
**摘要**：该研究克隆并表达了猪链球菌(*Streptococcus suis*)的SNTb1蛋白，通过原核表达系统获得重组蛋白，并验证其作为疫苗候选抗原的潜力，实验显示重组SNTb1能诱导小鼠产生特异性抗体。
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2. **文献名称**：*Recombinant SNTb1 adhesin of *Salmonella enterica* promotes bacterial adherence to host cells*
**作者**：García-Quintanilla M, et al.
**摘要**：研究通过大肠杆菌表达系统制备了沙门氏菌SNTb1黏附素的重组蛋白，证实其在细菌与宿主细胞黏附中的关键作用，并解析了其结构域与宿主受体的相互作用机制。
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3. **文献名称**：*Functional characterization of SNTb1 in biofilm formation of *Staphylococcus aureus* using recombinant protein technology*
**作者**：Chen X, et al.
**摘要**：利用重组SNTb1蛋白研究金黄色葡萄球菌生物膜形成机制，发现该蛋白通过调控多糖合成促进生物膜成熟，为抗感染治疗提供了新靶点。
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若需更多文献或具体数据库链接，可进一步补充关键词或研究领域。
**Background of SNTb1 Recombinant Protein**
SNTb1 (Staphylococcal Nuclease-Targeting Protein b1) is a recombinant protein derived from *Staphylococcus aureus*, a Gram-positive pathogen associated with diverse infections, including skin abscesses, bacteremia, and biofilm-related diseases. SNTb1 is a truncated or modified form of native staphylococcal nuclease (SNase), a secreted enzyme critical for S. aureus virulence. SNase facilitates immune evasion by degrading neutrophil extracellular traps (NETs) and extracellular DNA, thereby promoting bacterial survival in host environments.
The recombinant SNTb1 protein is typically engineered to retain functional domains essential for nuclease activity or protein-protein interactions while omitting regions linked to toxicity or instability. It is commonly produced in *E. coli* expression systems, followed by purification via affinity chromatography. This approach ensures high yield and purity, making SNTb1 a valuable tool for studying S. aureus pathogenesis, particularly mechanisms of immune evasion and biofilm dispersion.
Research applications include enzymatic assays to characterize nuclease functionality, structural studies to map binding sites, and immunological studies to evaluate host responses. SNTb1 also serves as an antigen in vaccine development or diagnostic kits aimed at detecting S. aureus infections. Additionally, its role in biofilm degradation has spurred interest in therapeutic strategies targeting chronic infections.
Overall, SNTb1 recombinant protein bridges basic and applied research, offering insights into staphylococcal biology while supporting efforts to develop novel antimicrobials and diagnostics. Its study underscores the importance of bacterial nucleases in infection dynamics and highlights potential targets for intervention.
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