| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PLCb3 |
| Uniprot No | Q01970 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1234aa |
| 氨基酸序列 | MAGAQPGVHALQLEPPTVVETLRRGSKFIKWDEETSSRNLVTLRVDPNGFFLYWTGPNMEVDTLDISSIRDTRTGRYARLPKDPKIREVLGFGGPDARLEEKLMTVVSGPDPVNTVFLNFMAVQDDTAKVWSEELFKLAMNILAQNASRNTFLRKAYTKLKLQVNQDGRIPVKNILKMFSADKKRVETALESCGLKFNRSESIRPDEFSLEIFERFLNKLCLRPDIDKILLEIGAKGKPYLTLEQLMDFINQKQRDPRLNEVLYPPLRPSQARLLIEKYEPNQQFLERDQMSMEGFSRYLGGEENGILPLEALDLSTDMTQPLSAYFINSSHNTYLTAGQLAGTSSVEMYRQALLWGCRCVELDVWKGRPPEEEPFITHGFTMTTEVPLRDVLEAIAETAFKTSPYPVILSFENHVDSAKQQAKMAEYCRSIFGDALLIEPLDKYPLAPGVPLPSPQDLMGRILVKNKKRHRPSAGGPDSAGRKRPLEQSNSALSESSAATEPSSPQLGSPSSDSCPGLSNGEEVGLEKPSLEPQKSLGDEGLNRGPYVLGPADREDEEEDEEEEEQTDPKKPTTDEGTASSEVNATEEMSTLVNYIEPVKFKSFEAARKRNKCFEMSSFVETKAMEQLTKSPMEFVEYNKQQLSRIYPKGTRVDSSNYMPQLFWNVGCQLVALNFQTLDVAMQLNAGVFEYNGRSGYLLKPEFMRRPDKSFDPFTEVIVDGIVANALRVKVISGQFLSDRKVGIYVEVDMFGLPVDTRRKYRTRTSQGNSFNPVWDEEPFDFPKVVLPTLASLRIAAFEEGGKFVGHRILPVSAIRSGYHYVCLRNEANQPLCLPALLIYTEASDYIPDDHQDYAEALINPIKHVSLMDQRARQLAALIGESEAQAGQETCQDTQSQQLGSQPSSNPTPSPLDASPRRPPGPTTSPASTSLSSPGQRDDLIASILSEVAPTPLDELRGHKALVKLRSRQERDLRELRKKHQRKAVTLTRRLLDGLAQAQAEGRCRLRPGALGGAADVEDTKEGEDEAKRYQEFQNRQVQSLLELREAQVDAEAQRRLEHLRQALQRLREVVLDANTTQFKRLKEMNEREKKELQKILDRKRHNSISEAKMRDKHKKEAELTEINRRHITESVNSIRRLEEAQKQRHDRLVAGQQQVLQQLAEEEPKLLAQLAQECQEQRARLPQEIRRSLLGEMPEGLGDGPLVACASNGHAPGSSGHLSGADSESQEENTQL |
| 预测分子量 | 138,7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PLCβ3重组蛋白的3篇文献示例(注：示例内容为虚构，仅作格式参考)：
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1. **标题**：Expression and Purification of Recombinant Phospholipase C β3 in Baculovirus System
**作者**：Kim, J. et al.
**摘要**：该研究利用杆状病毒表达系统成功表达了重组PLCβ3蛋白，并通过亲和层析实现了高效纯化。纯化后的蛋白在体外表现出磷脂水解活性，为后续功能研究提供了基础。
2. **标题**：Structural Insights into PLCβ3 Activation by Gαq
**作者**：Sutton, R.B. et al.
**摘要**：通过X射线晶体学解析了重组PLCβ3与Gαq蛋白复合物的结构，揭示了Gαq如何激活PLCβ3的催化结构域，阐明了其在G蛋白信号通路中的分子机制。
3. **标题**：Role of Recombinant PLCβ3 in Immune Cell Signaling
**作者**：Wang, L. et al.
**摘要**：研究利用重组PLCβ3蛋白分析了其在T细胞中的功能，发现其通过调节IP3-Ca²⁺信号通路影响细胞活化，为免疫相关疾病提供了潜在治疗靶点。
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(注：以上文献信息为模拟示例，实际引用请查询PubMed、Google Scholar等数据库获取真实文献。)
**Background of PLCβ3 Recombinant Protein**
Phospholipase C beta 3 (PLCβ3) is a critical enzyme in intracellular signaling, primarily involved in hydrolyzing phosphatidylinositol 4.5-bisphosphate (PIP₂) into secondary messengers inositol 1.4.5-trisphosphate (IP₃) and diacylglycerol (DAG). These molecules regulate calcium release and protein kinase C activation, respectively, influencing processes like cell proliferation, differentiation, and immune responses. PLCβ3 is one of four β isoforms (β1–β4) and is uniquely regulated by G-protein-coupled receptors (GPCRs) via Gαq/11 subunits, linking extracellular signals to intracellular pathways.
Dysregulation of PLCβ3 is implicated in diseases such as cancer, immune disorders, and neurological conditions. For instance, reduced PLCβ3 expression has been observed in certain cancers, suggesting tumor-suppressive roles. Conversely, hyperactivity may contribute to inflammation or autoimmune diseases. Studying PLCβ3’s structure-function relationships and interaction networks is vital for understanding its pathophysiological roles.
Recombinant PLCβ3 protein, produced using expression systems like *E. coli* or mammalian cells, enables researchers to investigate its enzymatic activity, regulatory mechanisms, and interactions *in vitro*. The recombinant form often includes tags (e.g., His-tag) for purification and detection. Its applications span drug discovery (e.g., targeting GPCR pathways), structural studies (e.g., crystallography), and functional assays to dissect signaling anomalies in disease models.
Overall, PLCβ3 recombinant protein serves as a key tool for decoding cellular signaling and developing therapeutic strategies for PLCβ3-associated disorders.
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