| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CHRNa2 |
| Uniprot No | Q15822 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-529aa |
| 氨基酸序列 | MGPSCPVFLSFTKLSLWWLLLTPAGGEEAKRPPPRAPGDPLSSPSPTALPQGGSHTETEDRLFKHLFRGYNRWARPVPNTSDVVIVRFGLSIAQLIDVDEKNQMMTTNVWLKQEWSDYKLRWNPTDFGNITSLRVPSEMIWIPDIVLYNNADGEFAVTHMTKAHLFSTGTVHWVPPAIYKSSCSIDVTFFPFDQQNCKMKFGSWTYDKAKIDLEQMEQTVDLKDYWESGEWAIVNATGTYNSKKYDCCAEIYPDVTYAFVIRRLPLFYTINLIIPCLLISCLTVLVFYLPSDCGEKITLCISVLLSLTVFLLLITEIIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHHRSPSTHTMPHWVRGALLGCVPRWLLMNRPPPPVELCHPLRLKLSPSYHWLESNVDAEEREVVVEEEDRWACAGHVAPSVGTLCSHGHLHSGASGPKAEALLQEGELLLSPHMQKALEGVHYIADHLRSEDADSSVKEDWKYVAMVIDRIFLWLFIIVCFLGTIGLFLPPFLAGMI |
| 预测分子量 | 59,7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CHRNA2重组蛋白的模拟参考文献示例，涵盖不同研究方向：
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1. **标题**: "Functional Expression of Recombinant CHRNA2 in Xenopus Oocytes Reveals Altered Pharmacological Properties"
**作者**: Tanaka M, et al.
**摘要**: 研究在非洲爪蟾卵母细胞中表达了重组CHRNA2蛋白，利用双电极电压钳技术证明其与β4亚基共表达时，对尼古丁的敏感性显著高于其他亚型组合，提示亚基特异性功能差异。
2. **标题**: "Cryo-EM Structure of the Human CHRNA2/LYNX1 Complex Modulates Ion Channel Gating"
**作者**: Chen H, et al.
**摘要**: 通过冷冻电镜解析CHRNA2重组蛋白与调控蛋白LYNX1的复合物结构，揭示了LYNX1如何通过结合胞外域变构调节离子通道的门控动力学，为神经信号调控机制提供新见解。
3. **标题**: "High-Yield Production of CHRNA2 Recombinant Protein in Insect Cells for Autoantibody Detection in Myasthenia Gravis"
**作者**: Rossi S, et al.
**摘要**: 开发基于杆状病毒系统的CHRNA2重组蛋白高效表达方法，应用于重症肌无力患者血清中特异性自身抗体的检测，显著提升诊断灵敏度。
4. **标题**: "CHRNA2 Knockout Mice with Recombinant Protein Rescue Exhibit Deficits in Auditory Processing"
**作者**: Kim T, et al.
**摘要**: 构建CHRNA2基因敲除小鼠模型，并通过脑室内注射重组蛋白部分恢复听觉皮层突触传递异常，证实CHRNA2在内源性听觉通路中的关键作用。
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*注：以上文献为示例性内容，实际研究中请通过学术数据库(如PubMed、Web of Science)检索真实发表论文。*
**Background of CHRNA2 Recombinant Protein**
The CHRNA2 gene encodes the α2 subunit of neuronal nicotinic acetylcholine receptors (nAChRs), a family of ligand-gated ion channels critical for synaptic transmission and neuromodulation. These receptors are composed of five subunits arranged around a central pore, with α2-containing receptors typically forming heteromeric complexes (e.g., α2β4) or, less commonly, homomeric configurations. nAChRs are widely expressed in the central and peripheral nervous systems, playing roles in cognitive functions, neurotransmitter release, and neural plasticity.
CHRNA2 has garnered attention due to its association with neurological and psychiatric disorders. Genetic studies link CHRNA2 variants to epilepsy, schizophrenia, and attention-deficit/hyperactivity disorder (ADHD), highlighting its importance in maintaining excitatory-inhibitory balance. The α2 subunit’s specific contribution to receptor kinetics, calcium permeability, and ligand sensitivity remains an active area of research, particularly in understanding how mutations disrupt neural circuits.
Recombinant CHRNA2 protein is produced using expression systems like *E. coli* or mammalian cell lines (e.g., HEK293), enabling controlled study of its structure-function relationships. Purified recombinant proteins facilitate *in vitro* assays, drug screening, and antibody development. They are essential for characterizing receptor pharmacology, mapping binding sites for toxins or therapeutics (e.g., nicotine, varenicline), and studying pathogenic mutations.
Advances in structural biology, such as cryo-EM, have utilized recombinant CHRNA2 to resolve receptor architectures, providing insights into gating mechanisms and allosteric modulation. This protein also serves as a tool for developing targeted therapies for nAChR-related disorders. Overall, CHRNA2 recombinant proteins are vital for bridging genetic findings to functional and translational research in neurobiology.
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