| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GRIK2 |
| Uniprot No | Q13002-5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-561aa |
| 氨基酸序列 | MKIIFPILSN PVFRRTVKLL LCLLWIGYSQ GTTHVLRFGG IFEYVESGPM GAEELAFRFA VNTINRNRTL LPNTTLTYDT QKINLYDSFE ASKKACDQLS LGVAAIFGPS HSSSANAVQS ICNALGVPHI QTRWKHQVSD NKDSFYVSLY PDFSSLSRAI LDLVQFFKWK TVTVVYDDST GLIRLQELIK APSRYNLRLK IRQLPADTKD AKPLLKEMKR GKEFHVIFDC SHEMAAGILK QALAMGMMTE YYHYIFTTLD LFALDVEPYR YSGVNMTGFR ILNTENTQVS SIIEKWSMER LQAPPKPDSG LLDGFMTTDA ALMYDAVHVV SVAVQQFPQM TVSSLQCNRH KPWRFGTRFM SLIKEAHWEG LTGRITFNKT NGLRTDFDLD VISLKEEGLE KIGTWDPASG LNMTESQKGK PANITDSLSN RSLIVTTILE EPYVLFKKSD KPLYGNDRFE GYCIDLLREL STILGFTYEI RLVEDGKYGA QDDANGQWNG MVRELIDHKA DLAVAPLAIT YVREKVIDFS KPFMTLGISI LYRKPNGTNP GVFSFLNPLS P |
| 预测分子量 | 87 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GRIK2重组蛋白的3篇代表性文献的简要概括(文献标题和作者为虚构示例，仅供格式参考)：
1. **标题**: "Expression and functional characterization of recombinant GRIK2 in HEK293 cells"
**作者**: Smith A et al.
**摘要**: 研究利用HEK293细胞表达GRIK2重组蛋白，验证其谷氨酸结合活性及通道门控特性，为体外研究红藻氨酸受体功能提供工具。
2. **标题**: "Structural insights into GRIK2 ligand-binding domain using X-ray crystallography"
**作者**: Chen L et al.
**摘要**: 解析GRIK2配体结合域(LBD)的晶体结构，揭示其与拮抗剂结合的关键氨基酸残基，助力靶向神经退行性疾病的药物设计。
3. **标题**: "GRIK2 knockout and recombinant rescue in autism spectrum disorder models"
**作者**: Wang Y et al.
**摘要**: 通过重组GRIK2蛋白回补实验，证明其在神经元突触可塑性中的作用，并关联自闭症模型中的行为异常机制。
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**注**：以上文献为示例模板，实际文献需通过PubMed/Google Scholar检索关键词如"GRIK2 recombinant protein"或"GluK2 expression"获取。
GRIK2 (Glutamate Ionotropic Receptor Kainate Type Subunit 2) is a protein-coding gene that plays a critical role in the central nervous system. It encodes the GluK2 subunit, a key component of kainate receptors (KARs), a subclass of ionotropic glutamate receptors (iGluRs). These receptors mediate fast excitatory neurotransmission and synaptic plasticity, influencing processes such as learning, memory, and neuronal development. GluK2-containing KARs are primarily located at pre- and postsynaptic sites, where they modulate neurotransmitter release and postsynaptic responses by permitting cation influx upon glutamate binding.
Recombinant GRIK2 protein is engineered in vitro to study the structure-function relationships, ligand interactions, and signaling mechanisms of kainate receptors. Researchers often express it in heterologous systems (e.g., HEK293 cells or Xenopus oocytes) to isolate its activity from other receptor subtypes. This allows precise investigation of its biophysical properties, such as ion selectivity, desensitization kinetics, and allosteric modulation by auxiliary proteins like Neto1/2.
Dysregulation of GRIK2 has been implicated in neurological and psychiatric disorders, including epilepsy, schizophrenia, and autism spectrum disorders. Recombinant GluK2 enables drug discovery efforts targeting these conditions, facilitating high-throughput screening of compounds aimed at modulating receptor activity. Additionally, structural studies using recombinant protein (e.g., cryo-EM or X-ray crystallography) have revealed binding sites for glutamate and allosteric modulators, advancing the design of selective therapeutics.
Despite progress, challenges remain in replicating native receptor complexes and post-translational modifications in recombinant systems. Ongoing research aims to refine expression platforms to better mimic physiological conditions, enhancing translational relevance for neurological disease research.
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