| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DOK1 |
| Uniprot No | Q99704 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-481aa |
| 氨基酸序列 | MDGAVMEGPLFLQSQRFGTKRWRKTWAVLYPASPHGVARLEFFDHKGSSSGGGRGSSRRLDCKVIRLAECVSVAPVTVETPPEPGATAFRLDTAQRSHLLAADAPSSAAWVQTLCRNAFPKGSWTLAPTDNPPKLSALEMLENSLYSPTWEGSQFWVTVQRTEAAERCGLHGSYVLRVEAERLTLLTVGAQSQILEPLLSWPYTLLRRYGRDKVMFSFEAGRRCPSGPGTFTFQTAQGNDIFQAVETAIHRQKAQGKAGQGHDVLRADSHEGEVAEGKLPSPPGPQELLDSPPALYAEPLDSLRIAPCPSQDSLYSDPLDSTSAQAGEGVQRKKPLYWDLYEHAQQQLLKAKLTDPKEDPIYDEPEGLAPVPPQGLYDLPREPKDAWWCQARVKEEGYELPYNPATDDYAVPPPRSTKPLLAPKPQGPAFPEPGTATGSGIKSHNSALYSQVQKSGASGSWDCGLSRVGTDKTGVKSEGST |
| 预测分子量 | 79 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DOK1重组蛋白的模拟参考文献示例(注：以下内容为虚构示例，实际文献需通过学术数据库查询)：
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1. **文献名称**: "Structural and Functional Characterization of Recombinant DOK1 Protein in T-Cell Signaling"
**作者**: Smith A, et al.
**摘要**: 本研究通过大肠杆菌表达系统成功制备了重组DOK1蛋白，解析了其酪氨酸磷酸化结构域的关键作用，并证明其在T细胞受体信号通路中负向调控MAPK激活的分子机制。
2. **文献名称**: "DOK1 Recombinant Protein Suppresses Tumor Growth in Colorectal Cancer Models"
**作者**: Lee H, et al.
**摘要**: 文章报道了重组DOK1蛋白通过抑制Ras-ERK信号通路，显著降低结直肠癌细胞增殖和体内移植瘤的生长，提示其作为潜在肿瘤治疗靶点的价值。
3. **文献名称**: "Development of a High-Yield DOK1 Recombinant Protein Production System Using Mammalian Cells"
**作者**: Garcia R, et al.
**摘要**: 研究者优化了哺乳动物细胞表达系统，实现重组DOK1蛋白的高效分泌表达，并验证其在体外实验中恢复DOK1缺失细胞的胰岛素信号敏感性功能。
4. **文献名称**: "DOK1 Phosphorylation Dynamics Analyzed by Recombinant Protein-Based Assays"
**作者**: Chen L, et al.
**摘要**: 利用重组DOK1蛋白建立体外磷酸化分析平台，揭示了Src家族激酶对DOK1不同酪氨酸位点的特异性修饰及其对下游信号复合体形成的影响。
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建议通过 **PubMed**、**Web of Science** 或 **Google Scholar** 检索关键词 "DOK1 recombinant protein" 或 "DOK1 expression" 获取真实文献。
**Background of DOK1 Recombinant Protein**
DOK1 (Docking Protein 1), also known as p62dok, is a key adaptor protein involved in regulating intracellular signaling pathways, particularly those linked to immune response, cell proliferation, and cancer. It belongs to the DOK family of proteins characterized by N-terminal pleckstrin homology (PH) and phosphotyrosine-binding (PTB) domains, along with C-terminal tyrosine phosphorylation sites. These structural features enable DOK1 to interact with phosphorylated tyrosine kinases and recruit downstream effectors, modulating signal transduction.
DOK1 was initially identified as a substrate of the BCR-ABL oncoprotein in chronic myelogenous leukemia (CML). It acts as a tumor suppressor by negatively regulating growth-promoting pathways, such as Ras-MAPK and PI3K-Akt, through interactions with kinases like Abl, Src, and insulin receptors. Loss or inactivation of DOK1 has been implicated in malignancies, including leukemia, lung cancer, and breast cancer, where its downregulation often correlates with hyperactivated oncogenic signaling and poor prognosis.
Recombinant DOK1 protein is engineered for *in vitro* and *in vivo* studies to dissect its molecular mechanisms. Produced using systems like *E. coli* or mammalian cells, it retains functional domains for binding assays, phosphorylation studies, or inhibitor screening. Its applications span basic research—exploring immune regulation, cancer biology, and metabolic signaling—to drug discovery, where it aids in validating therapeutic targets. Additionally, recombinant DOK1 serves as an antigen for antibody development, supporting diagnostic and functional studies.
Overall, DOK1 recombinant protein is a vital tool for unraveling its dual role as a signaling modulator and tumor suppressor, offering insights into disease mechanisms and potential therapies.
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