| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TUFT |
| Uniprot No | Q9NNX1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-390aa |
| 氨基酸序列 | MNGTRNWCTLVDVHPEDQAAGSVDILRLTLQGELTGDELEHIAQKAGRKTYAMVSSHSAGHSLASELVESHDGHEEIIKVYLKGRSGDKMIHEKNINQLKSEVQYIQEARNCLQKLREDISSKLDRNLGDSLHRQEIQVVLEKPNGFSQSPTALYSSPPEVDTCINEDVESLRKTVQDLLAKLQEAKRQHQSDCVAFEVTLSRYQREAEQSNVALQREEDRVEQKEAEVGELQRRLLGMETEHQALLAKVREGEVALEELRSNNADCQAEREKAATLEKEVAGLREKIHHLDDMLKSQQRKVRQMIEQLQNSKAVIQSKDATIQELKEKIAYLEAENLEMHDRMEHLIEKQISHGNFSTQARAKTENPGSIRISKPPSPKPMPVIRVVET |
| 预测分子量 | 44,2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TUFT(tuftelin)重组蛋白研究的示例参考文献。请注意，这些文献信息为示例性质，建议通过学术数据库(如PubMed、Google Scholar)获取真实文献：
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1. **标题**: *"Recombinant Tuftelin Expression and Its Role in Enamel Matrix Assembly"*
**作者**: Deutsch D., et al.
**摘要**: 研究报道了通过大肠杆菌表达系统成功重组表达人源tuftelin蛋白，并验证其在牙釉质基质自组装中的关键作用，揭示了其与amelogenin的相互作用机制。
2. **标题**: *"Biomineralization Potential of Recombinant Tuftelin in vitro"*
**作者**: Paine M.L., et al.
**摘要**: 通过体外实验证明重组tuftelin可诱导羟基磷灰石结晶，支持其在牙釉质生物矿化中的调控功能，为仿生材料开发提供理论依据。
3. **标题**: *"Functional Analysis of Recombinant Tuftelin in Cell Adhesion Signaling"*
**作者**: Smith J., et al.
**摘要**: 利用哺乳动物细胞表达重组tuftelin，发现其通过整合素信号通路促进成釉细胞黏附，提示其在牙发育中的多效性作用。
4. **标题**: *"Optimization of Tuftelin Production in Pichia pastoris for Biomedical Applications"*
**作者**: Zhang Y., et al.
**摘要**: 采用毕赤酵母系统优化重组tuftelin表达条件，提高蛋白产量及稳定性，探索其在牙组织工程中的应用潜力。
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建议通过关键词 **"recombinant tuftelin protein"** 或 **"tuftelin expression"** 在学术平台检索最新文献，以获取具体研究数据。
**Background of TUFT (Tuftelin) Recombinant Protein**
Tuftelin (TUFT) is an acidic, hydrophilic protein first identified in the enamel matrix of developing teeth. It plays a critical role in enamel biomineralization, interacting with other enamel-specific proteins like amelogenin to regulate hydroxyapatite crystal formation. Structurally, tuftelin contains multiple functional domains, including calcium-binding motifs and phosphorylation sites, which contribute to its role in extracellular matrix organization and mineralization processes.
The gene encoding tuftelin (*TUFT1*) is highly conserved across vertebrates, underscoring its evolutionary importance in dental development. Beyond its dental role, tuftelin has been detected in non-enamel tissues, such as bone, brain, and certain cancers, suggesting potential multifunctional roles in cell signaling, adhesion, or pathological calcification.
Recombinant tuftelin protein is produced using biotechnological platforms (e.g., bacterial, yeast, or mammalian expression systems) to enable detailed functional studies. Purified recombinant TUFT retains its native structural and biochemical properties, making it invaluable for *in vitro* studies on enamel formation, biomaterial design, and disease mechanisms. Researchers also use it to investigate TUFT's interactions with other proteins or ions, such as calcium and phosphate, to mimic natural mineralization processes.
Interest in TUFT recombinant protein has grown in regenerative dentistry and biomedicine, particularly for repairing enamel defects or engineering mineralized tissues. Challenges remain in optimizing its stability and activity in synthetic environments. Nonetheless, TUFT recombinant protein serves as a pivotal tool for unraveling biomineralization pathways and developing novel therapeutic strategies.
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