| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PTPRA |
| Uniprot No | P18433 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 20-151aa |
| 氨基酸序列 | NNATTVAPSVGITRLINSSTAEPVKEEAKTSNPTSSLTSLSVAPTFSPNITLGPTYLTTVNSSDSDNGTTRTASTNSIGITISPNGTWLPDNQFTDARTEPWEGNSSTAATTPETFPPSGNSDSKDRRDETP |
| 预测分子量 | 15.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PTPRA重组蛋白的3篇参考文献示例(注：以下内容为模拟生成，实际引用时请核实文献准确性)：
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1. **标题**: *"Crystal structure of the extracellular domain of protein tyrosine phosphatase receptor type A (PTPRA)"*
**作者**: Bhattacharyya, S., et al.
**摘要**: 报道了PTPRA胞外结构域的晶体结构，揭示其免疫球蛋白样折叠模式，为研究PTPRA在细胞粘附和信号传导中的作用提供结构基础。
2. **标题**: *"PTPRA regulates EGFR activation by dephosphorylating the kinase domain"*
**作者**: Tarcic, G., et al.
**摘要**: 通过重组PTPRA蛋白的体外实验，证明其直接与EGFR激酶结构域结合并去磷酸化，抑制EGFR激活及下游肿瘤细胞增殖信号。
3. **标题**: *"Recombinant PTPRA phosphatase activity is modulated by phosphorylation of a conserved serine residue"*
**作者**: Chen, Y., et al.
**摘要**: 发现重组PTPRA的Ser189位点磷酸化可抑制其磷酸酶活性，提示翻译后修饰对PTPRA功能的调控机制。
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(如需第四篇示例，可补充：)
4. **标题**: *"Expression and purification of active PTPRA in mammalian cells for neuronal signaling studies"*
**作者**: Müller, T., et al.
**摘要**: 开发了一种哺乳动物细胞表达系统，高效纯化具有酶活性的重组PTPRA，并验证其在神经元轴突生长中的调控作用。
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建议通过PubMed或Google Scholar检索最新文献，使用关键词“PTPRA recombinant protein”“PTPRA phosphatase activity”等进一步筛选。
**Background of PTPRA Recombinant Protein**
Protein Tyrosine Phosphatase Receptor Type A (PTPRA), also known as RPTPα, is a member of the protein tyrosine phosphatase (PTP) family, which plays critical roles in regulating cellular signaling by dephosphorylating tyrosine residues on target proteins. PTPRA is a transmembrane receptor-type phosphatase characterized by an extracellular ligand-binding domain, a single transmembrane region, and two intracellular catalytic domains. It is widely expressed in various tissues, including the brain, liver, and immune cells, and is implicated in modulating cell adhesion, growth, differentiation, and oncogenic signaling pathways.
PTPRA is particularly notable for its role in regulating Src family kinases (SFKs), such as c-Src and Fyn, by dephosphorylating inhibitory phosphotyrosine residues to activate their kinase activity. This interaction links PTPRA to critical processes like mitogenic signaling, cytoskeletal reorganization, and cell cycle progression. Dysregulation of PTPRA has been associated with cancers, metabolic disorders, and neurological diseases, highlighting its therapeutic and diagnostic potential.
Recombinant PTPRA protein is produced via genetic engineering in heterologous systems (e.g., *E. coli*, mammalian cells) to ensure high purity and functional activity. This engineered protein retains the enzymatic properties of native PTPRA, enabling studies on its substrate specificity, signaling mechanisms, and interactions with partners. Researchers use recombinant PTPRA to investigate its role in disease pathways, screen for inhibitors/activators, and develop targeted therapies. Its application extends to structural studies, antibody production, and elucidating phosphorylation-dependent signaling networks in health and disease.
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