| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SDF2L1 |
| Uniprot No | Q9HCN8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 29-221aa |
| 氨基酸序列 | AK TGAELVTCGS VLKLLNTHHR VRLHSHDIKY GSGSGQQSVT GVEASDDANS YWRIRGGSEG GCPRGSPVRC GQAVRLTHVL TGKNLHTHHF PSPLSNNQEV SAFGEDGEGD DLDLWTVRCS GQHWEREAAV RFQHVGTSVF LSVTGEQYGS PIRGQHEVHG MPSANTHNTW KAMEGIFIKP SVEPSAGHDE L |
| 预测分子量 | 23,5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SDF2L1重组蛋白的构造参考文献示例(建议通过学术数据库验证具体文献)：
---
1. **文献名称**: *Recombinant SDF2L1 Protein Production in E. coli: Purification and Functional Characterization*
**作者**: Tanaka A, et al.
**摘要**: 本研究报道了在大肠杆菌中高效表达重组人SDF2L1蛋白的方法，通过优化密码子和纯化步骤获得高纯度蛋白。功能实验表明，重组SDF2L1可增强内质网应激下细胞的存活率，提示其作为分子伴侣的潜在作用。
2. **文献名称**: *Structural Insights into SDF2L1’s Role in Protein Quality Control*
**作者**: Müller S, et al.
**摘要**: 通过X射线晶体学解析了重组SDF2L1蛋白的三维结构，揭示了其与内质网定位相关的保守结构域。进一步实验证明，SDF2L1与热休克蛋白HSP47相互作用，共同参与胶原蛋白的折叠调控。
3. **文献名称**: *SDF2L1 Recombinant Protein Attenuates Fibrosis in a Mouse Model of Systemic Sclerosis*
**作者**: Chen L, et al.
**摘要**: 利用哺乳动物细胞表达系统制备重组小鼠SDF2L1蛋白，并在系统性硬化症模型中进行验证。结果显示，SDF2L1治疗显著减少皮肤和肺组织纤维化，机制涉及TGF-β信号通路抑制。
---
**备注**：以上文献为基于领域常见研究的构造示例，实际文献需通过PubMed、Google Scholar等平台以“SDF2L1 recombinant protein”或“SDF2L1 function”等关键词检索获取。
**Background of SDF2L1 Recombinant Protein**
SDF2L1 (Stromal Cell-Derived Factor 2-Like 1) is a member of the SDF2 protein family, which shares homology with stromal cell-derived factors involved in cellular stress responses and protein quality control. This conserved eukaryotic protein is localized primarily in the endoplasmic reticulum (ER) and plays a role in modulating ER stress pathways, particularly during the unfolded protein response (UPR). SDF2L1 is implicated in assisting proper protein folding, degradation of misfolded proteins, and maintaining ER homeostasis, making it critical for cellular survival under stress conditions.
Structurally, SDF2L1 contains an N-terminal signal peptide, a conserved SDF2 domain, and potential glycosylation sites. Its recombinant form is engineered using expression systems (e.g., *E. coli*, mammalian, or insect cells*) to produce a purified, bioactive protein for research applications. Recombinant SDF2L1 retains the functional domains necessary for interacting with ER chaperones like HSPA5/BiP and components of the UPR machinery, such as IRE1α and PERK pathways.
Research on SDF2L1 has linked it to various physiological and pathological processes. It is upregulated in conditions involving ER stress, including cancer, neurodegenerative diseases (e.g., Alzheimer’s), and metabolic disorders. In cancer, SDF2L1 may promote tumor cell survival under hypoxic or nutrient-deprived microenvironments. Conversely, its dysregulation is associated with disrupted protein secretion pathways in genetic diseases.
The recombinant SDF2L1 protein serves as a tool to study ER stress mechanisms, protein-protein interactions, and therapeutic targeting. It is also explored for potential applications in drug screening or as a biomarker for stress-related pathologies. Ongoing studies aim to clarify its precise molecular roles and therapeutic relevance, highlighting its importance in cellular stress adaptation and disease progression.
×
