| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PTPRE |
| Uniprot No | P23469 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 75-700aa |
| 氨基酸序列 | RKAVVSTSDKKMPNGILEEQEQQRVMLLSRSPSGPKKYFPIPVEHLEEEIRIRSADDCKQFREEFNSLPSGHIQGTFELANKEENREKNRYPNILPNDHSRVILSQLDGIPCSDYINASYIDGYKEKNKFIAAQGPKQETVNDFWRMVWEQKSATIVMLTNLKERKEEKCHQYWPDQGCWTYGNIRVCVEDCVVLVDYTIRKFCIQPQLPDGCKAPRLVSQLHFTSWPDFGVPFTPIGMLKFLKKVKTLNPVHAGPIVVHCSAGVGRTGTFIVIDAMMAMMHAEQKVDVFEFVSRIRNQRPQMVQTDMQYTFIYQALLEYYLYGDTELDVSSLEKHLQTMHGTTTHFDKIGLEEEFRKLTNVRIMKENMRTGNLPANMKKARVIQIIPYDFNRVILSMKRGQEYTDYINASFIDGYRQKDYFIATQGPLAHTVEDFWRMIWEWKSHTIVMLTEVQEREQDKCYQYWPTEGSVTHGEITIEIKNDTLSEAISIRDFLVTLNQPQARQEEQVRVVRQFHFHGWPEIGIPAEGKGMIDLIAAVQKQQQQTGNHPITVHCSAGAGRTGTFIALSNILERVKAEGLLDVFQAVKSLRLQRPHMVQTLEQYEFCYKVVQDFIDIFSDYANFK |
| 预测分子量 | 91 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于PTPRE重组蛋白研究的参考文献概览：
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1. **文献名称**：*Structural and Functional Characterization of Recombinant PTPRE Phosphatase Domain*
**作者**：Chen X, et al.
**摘要**：该研究通过大肠杆菌系统成功表达并纯化了人源PTPRE的催化结构域重组蛋白，解析其晶体结构，揭示了其底物结合特异性，并验证了其在胰岛素信号通路中去磷酸化调控的作用机制。
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2. **文献名称**：*PTPRE Regulates EGFR Activation and Promotes Tumor Metastasis via Recombinant Protein Interaction Studies*
**作者**：Wang L, et al.
**摘要**：通过构建PTPRE重组蛋白与表皮生长因子受体(EGFR)的体外结合实验，证明PTPRE通过直接去磷酸化EGFR抑制其活性，抑制肺癌细胞侵袭转移，为靶向治疗提供依据。
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3. **文献名称**：*Development of a High-Yield Mammalian Expression System for PTPRE and Its Role in Metabolic Disorders*
**作者**：Johnson R, et al.
**摘要**：利用哺乳动物细胞表达系统高效制备功能性PTPRE重组蛋白，发现其在肝脏细胞中调控胰岛素受体信号异常，与肥胖和Ⅱ型糖尿病发病相关。
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*注：以上文献为示例，实际引用需根据具体研究补充真实来源。建议通过PubMed或Web of Science以“PTPRE recombinant protein”为关键词检索最新论文。*
**Background of PTPRE Recombinant Protein**
Protein tyrosine phosphatase receptor type E (PTPRE), a member of the protein tyrosine phosphatase (PTP) family, plays a critical role in regulating cellular signaling by dephosphorylating tyrosine residues in target proteins. As a receptor-type PTP, it features an extracellular segment, a transmembrane domain, and two intracellular catalytic domains, enabling its involvement in bidirectional signal transduction across the plasma membrane. PTPRE is widely expressed in tissues, including the brain, liver, and immune cells, and is implicated in modulating pathways linked to insulin signaling, growth factor responses, and cytokine receptor activity.
Recombinant PTPRE proteins are engineered *in vitro* using expression systems (e.g., mammalian, bacterial, or insect cells) to produce purified, functional forms of the enzyme. These proteins retain the catalytic activity required for studying phosphatase mechanisms, substrate interactions, and regulatory roles in physiological processes. Research highlights PTPRE's dual role in diseases: it can act as a tumor suppressor by downregulating oncogenic kinases or promote pathologies like metabolic disorders by impairing insulin receptor signaling. Its involvement in immune regulation also links it to autoimmune diseases and inflammation.
Therapeutic targeting of PTPRE is explored in contexts such as cancer (via kinase signaling modulation) and diabetes (by enhancing insulin sensitivity). Recombinant PTPRE serves as a vital tool for structural studies, inhibitor screening, and antibody development. However, conflicting evidence regarding its pro- or anti-disease roles underscores the need for context-specific research. Overall, PTPRE recombinant proteins provide a versatile platform for dissecting tyrosine phosphorylation networks and advancing targeted therapies.
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