| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CKAP2 |
| Uniprot No | Q8WWK9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-683aa |
| 氨基酸序列 | MSTPAVPQDL QLPPSQRAQS AFKEQRRQKL KEHLLRRKTL FAYKQENEML SSSRDQRVVT SEDQVQEGTK VLKLKTKMAD KENMKRPAES KNNTVVGKHC IPLKPSNELT NSTVVIDTHK PKDSNQTPHL LLTEDDPQSQ HMTLSQAFHL KNNSKKKQMT TEKQKQDANM PKKPVLGSYR GQIVQSKINS FRKPLQVKDE SSAATKKLSA TIPKATKPQP VNTSSVTVKS NRSSNMTATT KFVSTTSQNT QLVRPPIRSH HSNTRDTVKQ GISRTSANVT IRKGPHEKEL LQSKTALSSV KTSSSQGIIR NKTLSRSIAS EVIARPASLS NDKLMEKSEP VDQRRHTAGK AIVDSRSAQP KETSEERKAR LSEWKAGKGR VLKRPPNSVV TQHEPAGQNE KPVGSFWTTM AEEDEQRLFT EKVNNTFSEC LNLINEGCPK EDILVTLNDL IKNIPDAKKL VKYWICLALI EPITSPIENI IAIYEKAILA GAQPIEEMRH TIVDILTMKS QEKANLGENM EKSCASKEEV KEVSIEDTGV DVDPEKLEME SKLHRNLLFQ DCEKEQDNKT KDPTHDVKTP NTETRTSCLI KYNVSTTPYL QSVKKKVQFD GTNSAFKELK FLTPVRRSRR LQEKTSKLPD MLKDHYPCVS SLEQLTELGR ETDAFVCRPN AALCRVYYEA DTT |
| 预测分子量 | 76,9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CKAP2重组蛋白的3篇参考文献示例(部分信息为模拟，供参考)：
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1. **文献名称**: "CKAP2 regulates mitotic spindle assembly and progression via interaction with microtubules"
**作者**: Tanaka R, et al.
**摘要**: 研究通过重组CKAP2蛋白揭示了其与微管的直接相互作用，证明其在有丝分裂纺锤体组装中的关键作用，并发现其异常表达可导致染色体分离错误。
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2. **文献名称**: "Recombinant CKAP2 promotes tumor cell proliferation in hepatocellular carcinoma through ERK signaling activation"
**作者**: Liu Y, Wang X, Zhang H.
**摘要**: 利用重组CKAP2蛋白进行体外实验，发现其通过激活ERK信号通路促进肝癌细胞增殖，提示CKAP2可能作为肝癌治疗的潜在靶点。
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3. **文献名称**: "Expression and purification of human CKAP2 in E. coli for functional characterization"
**作者**: Kim S, Park JH.
**摘要**: 报道了在大肠杆菌系统中高效表达和纯化重组CKAP2蛋白的方法，并通过体外微管结合实验验证了其生物活性。
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注：以上文献为示例，建议通过PubMed、Web of Science或Google Scholar以“CKAP2 recombinant protein”“CKAP2 expression”等关键词检索真实文献。
CKAP2 (cytoskeleton-associated protein 2), also known as LINC00598 or tumor-associated microtubule protein, is a protein implicated in cell cycle regulation, particularly during mitosis. It is structurally characterized by coiled-coil domains and is thought to play roles in microtubule dynamics, spindle assembly, or chromosome segregation. Dysregulation of CKAP2 has been linked to carcinogenesis, with overexpression observed in various cancers (e.g., breast, liver, lung), correlating with poor prognosis, enhanced proliferation, and metastasis. Its involvement in mitotic fidelity makes it a potential biomarker and therapeutic target.
Recombinant CKAP2 protein is engineered using heterologous expression systems (e.g., *E. coli*, mammalian cells) for functional studies. The purified protein enables *in vitro* investigations into its interactions with cytoskeletal components, post-translational modifications, and mechanisms driving oncogenicity. Researchers utilize it to screen small-molecule inhibitors or validate CRISPR/Cas9-mediated gene editing. Challenges in production include maintaining solubility due to its structural complexity and avoiding aggregation during purification. Recent studies focus on its role in cancer stemness and resistance to chemotherapeutics, with recombinant forms facilitating structural analysis via cryo-EM or X-ray crystallography. Despite progress, its exact molecular mechanisms remain partially unresolved, necessitating further research into its context-dependent roles in both normal and pathological states.
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